SPO74_YEAST
ID SPO74_YEAST Reviewed; 413 AA.
AC P45819; D6VTY2; E9P8T5;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Sporulation-specific protein 74;
GN Name=SPO74; OrderedLocusNames=YGL170C; ORFNames=G1654;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8896267;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<1033::aid-yea983>3.0.co;2-v;
RA Klima R., Coglievina M., Zaccaria P., Bertani I., Bruschi C.V.;
RT "A putative helicase, the SUA5, PMR1, tRNALys1 genes and four open reading
RT frames have been detected in the DNA sequence of an 8.8 kb fragment of the
RT left arm of chromosome VII of Saccharomyces cerevisiae.";
RL Yeast 12:1033-1040(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND INTERACTION WITH
RP SPO21 AND MPC54.
RX PubMed=12796288; DOI=10.1128/ec.2.3.431-445.2003;
RA Nickas M.E., Schwartz C., Neiman A.M.;
RT "Ady4p and Spo74p are components of the meiotic spindle pole body that
RT promote growth of the prospore membrane in Saccharomyces cerevisiae.";
RL Eukaryot. Cell 2:431-445(2003).
CC -!- FUNCTION: Involved in the pathway that organizes the shaping and sizing
CC of the prospore membrane (PSM) during sporulation. Probable component
CC of a core structural unit of the scaffold that initiates synthesis of
CC the prospore membrane. {ECO:0000269|PubMed:12796288}.
CC -!- SUBUNIT: Interacts with itself. Interacts with MPC54, NUD1 and
CC SPO21/MPC70. {ECO:0000269|PubMed:12796288}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, spindle pole body {ECO:0000269|PubMed:12796288}. Note=Localizes
CC to the meiotic outer plaque of the spindle pole body (SPB), at the end
CC of the meiotic spindles.
CC -!- DEVELOPMENTAL STAGE: Meiosis-specific. {ECO:0000269|PubMed:12796288}.
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DR EMBL; X85757; CAA59759.1; -; Genomic_DNA.
DR EMBL; Z72692; CAA96882.1; -; Genomic_DNA.
DR EMBL; AY557838; AAS56164.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA07943.1; -; Genomic_DNA.
DR PIR; S59650; S59650.
DR RefSeq; NP_011345.3; NM_001181035.3.
DR AlphaFoldDB; P45819; -.
DR SMR; P45819; -.
DR BioGRID; 33083; 133.
DR DIP; DIP-1458N; -.
DR IntAct; P45819; 5.
DR MINT; P45819; -.
DR STRING; 4932.YGL170C; -.
DR PaxDb; P45819; -.
DR PRIDE; P45819; -.
DR EnsemblFungi; YGL170C_mRNA; YGL170C; YGL170C.
DR GeneID; 852705; -.
DR KEGG; sce:YGL170C; -.
DR SGD; S000003138; SPO74.
DR VEuPathDB; FungiDB:YGL170C; -.
DR HOGENOM; CLU_060606_0_0_1; -.
DR InParanoid; P45819; -.
DR OMA; RSNEEMY; -.
DR BioCyc; YEAST:G3O-30658-MON; -.
DR PRO; PR:P45819; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P45819; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0035974; C:meiotic spindle pole body; IDA:SGD.
DR GO; GO:0005816; C:spindle pole body; HDA:SGD.
DR GO; GO:0005198; F:structural molecule activity; IDA:SGD.
DR GO; GO:0030437; P:ascospore formation; IMP:SGD.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Reference proteome; Sporulation.
FT CHAIN 1..413
FT /note="Sporulation-specific protein 74"
FT /id="PRO_0000072142"
FT REGION 1..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..60
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 69..87
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 328
FT /note="I -> V (in Ref. 4; AAS56164)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 413 AA; 47700 MW; ACEB8DA9A240B650 CRC64;
MGAGTLLNGL EKENFPNNIH SDLPAYPNMD SQEDGNTSKE SKRNSPVKQK SQKDEEKSSK
MGTASNIFHE NKDIHERSEH TDDFNDGLKL APDSSPSLKE CQFKNWESFW CNTEGYKTKH
MQPFHFTSGL EEIKEPVMEL NISTSPYKGQ RPNSAPTEYS AATTAFTKTQ LEVSFLKTNL
LTYIKKEIDI CLSSVPFFDD AVQMQKKFLE YRDIDLDEEY ELKILGELLN DLNFFHMQEN
SLLNRELAVR RFSNQPESQN LPSIRDFRNP LLPIDNRPSP PLGLKRNGKS FEETYDFTSN
TSNFWGEKAE LQNSITGGTP YFFHPNNIHQ TKPFMSFENQ NELLFQRKNS DYKQHFNSGR
NIHNGVESKS YRGVGLNDSY QKGYAAMTKS FGNIDLNRMP RRSNEEMYSW SRN
