SPOC1_MOUSE
ID SPOC1_MOUSE Reviewed; 1015 AA.
AC B1ASB6;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2021, sequence version 3.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=SPOC domain-containing protein 1;
GN Name=Spocd1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND INTERACTION WITH
RP DNMT3A; DNMT3C AND DNMT3L.
RX PubMed=32674113; DOI=10.1038/s41586-020-2557-5;
RA Zoch A., Auchynnikava T., Berrens R.V., Kabayama Y., Schoepp T., Heep M.,
RA Vasiliauskaite L., Perez-Rico Y.A., Cook A.G., Shkumatava A.,
RA Rappsilber J., Allshire R.C., O'Carroll D.;
RT "SPOCD1 is an essential executor of piRNA-directed de novo DNA
RT methylation.";
RL Nature 584:635-639(2020).
CC -!- FUNCTION: Essential excecutor of PIWIL4-piRNA pathway directed
CC transposon DNA methylation and silencing in the male embryonic germ
CC cells (PubMed:32674113). Associates with the de novo DNA methylation
CC machinery and repressive chromatin remodeling complexes
CC (PubMed:32674113). Tethering of PIWIL4 to a nascent transposable
CC element transcript recruits repressive chromatin remodeling activities
CC and the de novo methylation apparatus through SPOCD1 (PubMed:32674113).
CC Not required for piRNA biosynthesis (PubMed:32674113).
CC {ECO:0000269|PubMed:32674113}.
CC -!- SUBUNIT: Interacts with DNMT3A, DNMT3C and DNMT3L.
CC {ECO:0000269|PubMed:32674113}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:32674113}. Chromosome
CC {ECO:0000269|PubMed:32674113}.
CC -!- DEVELOPMENTAL STAGE: Detected in embryonic testis at 14.5 dpc and
CC decreases by postnatal day 5. {ECO:0000269|PubMed:32674113}.
CC -!- DISRUPTION PHENOTYPE: Male mice are infertile with spermatozoa
CC completely absent in the epididymis (PubMed:32674113). Male germ cells
CC show derepression of transposable elements (TEs) and DNA
CC hypomethylation of TEs (PubMed:32674113).
CC {ECO:0000269|PubMed:32674113}.
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DR EMBL; GL456106; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_017175994.1; XM_017320505.1.
DR AlphaFoldDB; B1ASB6; -.
DR SMR; B1ASB6; -.
DR STRING; 10090.ENSMUSP00000081284; -.
DR PhosphoSitePlus; B1ASB6; -.
DR PaxDb; B1ASB6; -.
DR PRIDE; B1ASB6; -.
DR Antibodypedia; 31194; 27 antibodies from 12 providers.
DR GeneID; 622480; -.
DR KEGG; mmu:622480; -.
DR CTD; 90853; -.
DR MGI; MGI:3652045; Spocd1.
DR VEuPathDB; HostDB:ENSMUSG00000028784; -.
DR eggNOG; KOG1634; Eukaryota.
DR HOGENOM; CLU_029996_0_0_1; -.
DR InParanoid; B1ASB6; -.
DR PhylomeDB; B1ASB6; -.
DR TreeFam; TF343963; -.
DR ChiTaRS; Spocd1; mouse.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; B1ASB6; protein.
DR ExpressionAtlas; B1ASB6; baseline and differential.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006306; P:DNA methylation; IMP:UniProtKB.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0010529; P:negative regulation of transposition; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR GO; GO:0006351; P:transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.472.30; -; 1.
DR InterPro; IPR012921; SPOC_C.
DR InterPro; IPR003618; TFIIS_cen_dom.
DR InterPro; IPR036575; TFIIS_cen_dom_sf.
DR Pfam; PF07744; SPOC; 1.
DR Pfam; PF07500; TFIIS_M; 1.
DR SMART; SM00510; TFS2M; 1.
DR SUPFAM; SSF46942; SSF46942; 1.
DR PROSITE; PS51321; TFIIS_CENTRAL; 1.
PE 1: Evidence at protein level;
KW Chromosome; Differentiation; Nucleus; Reference proteome;
KW RNA-mediated gene silencing; Spermatogenesis.
FT CHAIN 1..1015
FT /note="SPOC domain-containing protein 1"
FT /id="PRO_0000451971"
FT DOMAIN 410..530
FT /note="TFIIS central"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00651"
FT DOMAIN 688..791
FT /note="SPOC"
FT REGION 73..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 118..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 344..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 643..685
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 858..906
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 967..1015
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 286..320
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1015 AA; 110815 MW; 12986EDA44CA9A89 CRC64;
MPVDKFLARA GGQRTLSRFQ DSLCDFLATG SSRSLCGTSL DFPDRVLVCT EGPAGMEEAS
SPSFRQATTV EGMVSPEDPT LSKEGLSAKG PPPSPVLPPF RRKCRKCGIC PQEEEKSGFS
LRFGQRPDGD PPECASPEAF AGPPRLLSSK DTEPGEPGGS WVECAVETEV ENMLFSKDGV
APDSLDHLFE FPGLFGGEPL SLVTQEPLQS PALYPEVLTE DSTDQQEVQP AFVVGSPGTP
PDPVGPEDKA QSGSRMELEQ GLSAPANSCA SSPEPLGGLD GAPLEQRRPT HVKKRPWTSP
DDSSQDHAEG ASKKDFPRLE ARVPRGVKTV YYVYTGSGIR LVGAASSSQP GQIEPLEELD
TNSARRKRRP TTAHPTPCQS DPSETENAEP HQQRAEDPGE QGMTPLDAGV RSTVVRAMQE
VLWTRAQELP DLALREDEVE AIAEGIEEAL FHLTQDTNLR YKNKYRSLLF NLRDPRNVDL
FLKVAHCDVT PNNLVQMSSI QLAPKELSRW RDQEERKGLD IIEQQQKELY RLPASKLTHK
GEVEIPRDLD QMLTLEDLME PIVPRECSLQ PLSTPLEDTT NWHQHHQCDS NCQICTGGEE
GRKADDGSQP ELQVDISYNV DRKSSTKLPA FSGVIMNREE NAIQKAPGPA PASSPEVLKV
GETPPKEPQD RLQMPAGLKN APPSPPPWEG SLDMFSIKHF RAKAQLISGH SCQLVQALPD
VIRSAGRLPP SHVWDLLDSM GPSKAKDICV IRLCPHGSRD IQNYRLLYSY LNNKQCHCLA
TVQQVKMVLL PLPAFEPLPA RLRPLGGPGL EITHTSLLLA VLFPKDALPD TATSIPVSNK
VPKTVSFSKR VERILYSPED RRSEATSSPL EEDPKQSLAR GSLAPRSVCA PQSFPRGRGR
QGPGWGQWSP EAAWCYSQHP SSAGPVFPGI GQGQHLHRAS CFHHDLLQHL KVLVTMSHQF
QASLWPQSQD SLPPSTVVSA VPDPPGPSLG PMDGGGSNCP PPEGSDPLEP PEHEC
