SPOK1_PODCO
ID SPOK1_PODCO Reviewed; 734 AA.
AC A0A447CCJ8; A0A059PWG2;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 08-MAY-2019, sequence version 1.
DT 25-MAY-2022, entry version 9.
DE RecName: Full=Meiotic driver SPOK1 {ECO:0000305};
DE AltName: Full=Spore killer 1 {ECO:0000303|PubMed:24830502};
GN Name=SPOK1 {ECO:0000303|PubMed:24830502};
GN ORFNames=PODCO_500010 {ECO:0000312|EMBL:VBB81150.1};
OS Podospora comata.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Podosporaceae; Podospora.
OX NCBI_TaxID=48703;
RN [1] {ECO:0000312|Proteomes:UP000280685}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Td {ECO:0000305};
RX PubMed=24830502; DOI=10.1371/journal.pgen.1004387;
RA Grognet P., Lalucque H., Malagnac F., Silar P.;
RT "Genes that bias mendelian segregation.";
RL PLoS Genet. 10:E1004387-E1004387(2014).
RN [2] {ECO:0000312|Proteomes:UP000280685}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T {ECO:0000312|Proteomes:UP000280685};
RA Silar P.;
RL Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Td {ECO:0000303|PubMed:31347500};
RX PubMed=31347500; DOI=10.7554/elife.46454;
RA Vogan A.A., Ament-Velasquez S.L., Granger-Farbos A., Svedberg J.,
RA Bastiaans E., Debets A.J., Coustou V., Yvanne H., Clave C., Saupe S.J.,
RA Johannesson H.;
RT "Combinations of Spok genes create multiple meiotic drivers in Podospora.";
RL Elife 8:e46454-e46454(2019).
CC -!- FUNCTION: Promotes unequal transmission of alleles from the parental
CC zygote to progeny spores by acting as poison/antidote system, leading
CC to poisoning of progeny that do not inherit the allele
CC (PubMed:24830502, PubMed:31347500). Can suppress meiotic drive by the
CC P.anserina SPOK2, SPOK3 and SPOK4 proteins (PubMed:24830502,
CC PubMed:31347500). {ECO:0000269|PubMed:24830502,
CC ECO:0000269|PubMed:31347500}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2AFA8}. Nucleus
CC {ECO:0000250|UniProtKB:B2AFA8}.
CC -!- DEVELOPMENTAL STAGE: Present in both spores and vegetative cells.
CC {ECO:0000269|PubMed:31347500}.
CC -!- DISRUPTION PHENOTYPE: Sensitises spores to SPOK1 encoded poison.
CC {ECO:0000269|PubMed:24830502}.
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DR EMBL; LR026968; VBB81150.1; -; Genomic_DNA.
DR EMBL; JX560967; AGO59329.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A447CCJ8; -.
DR VEuPathDB; FungiDB:PODANS_5_10; -.
DR Proteomes; UP000280685; Chromosome 5.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0110134; P:meiotic drive; IMP:UniProtKB.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR SUPFAM; SSF56112; SSF56112; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; Nucleus; Toxin.
FT CHAIN 1..734
FT /note="Meiotic driver SPOK1"
FT /id="PRO_0000453035"
FT REGION 180..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 414..499
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 4..41
FT /evidence="ECO:0000255"
FT COMPBIAS 181..207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 414..451
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 455..471
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 734 AA; 83095 MW; 203C92FDCEDE2531 CRC64;
MSDKDRIAQL LRELEEAKAR VEEAKAREAQ ERCEKERLQL EHRKTTFLEY LRNCHRHLYN
ALRLTDTSRS STGYTKVVGK YYPKRLRPWT NFANVLHPRY FDLVQKICGQ RQLFEPASTT
KNLGTIISDH LAGNEKAIDR FEVDAVERPV QAILKVLATH EEAGKAYRCP EFRFSANLRE
LTQEDDGSSG ADDNTSDGSL ERRQQAGPNK RPTSKRKYIC SNRQPDGVGI RMQPGGGQTQ
AFIYDYKAAH KVAIEHVRSA TAKEHLFHEV VARINDNKLS RDKEVQRREQ AEAFIAMALT
QVFDYMITYG VSYGYVAAGR CLLLLYVDRD DWQTLYCHPC LPADDVGEPT NDWTDRLSHT
AVAQLVSFCL SSFQSEALEG QSLETALSVA NATLKTWSES YADVAYLGLE PAELSSAPSS
QNTDISEYTS KAKPTGRNVA LRSQSSCKPA AVLPQGNEHD EHDEDHSEPG ASRSRLAANK
RKRGPSSGGE DEDIAMADPE PTRQYCTQAC LLGLKRGKDL DENCPNVSLH RFDGSSRHPV
NAHRFTDMVK QQLLLSPYKG CRMVDFWGKR GAMGWLFKLE LFPYGYTFVG KGTLEDRLSR
LEHEGRVYAR LDHLQGDVVP VHLGLVRLDR GYILPGLEFV VYMMLMSWAG QTPSASMADA
ETLKRESLTA IWSEGVDHGD DNRANYLWNA ERCRIMIIDF DRARLFPPLK PRAVSRLSKP
KRERGNRSRH QIRT