SPOK2_PODAN
ID SPOK2_PODAN Reviewed; 775 AA.
AC B2AFA8; A0A090CKU7;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 02-JUN-2021, sequence version 2.
DT 25-MAY-2022, entry version 48.
DE RecName: Full=Meiotic driver SPOK2 {ECO:0000305};
DE AltName: Full=Spore killer 2 {ECO:0000303|PubMed:24830502};
GN Name=SPOK2 {ECO:0000303|PubMed:24830502};
GN ORFNames=PODANS_5_10 {ECO:0000312|EMBL:CAP62126.1};
OS Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383)
OS (Pleurage anserina).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Podosporaceae; Podospora;
OC Podospora anserina.
OX NCBI_TaxID=515849 {ECO:0000312|EMBL:CAP62126.1};
RN [1] {ECO:0000312|EMBL:CAP62126.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S / ATCC MYA-4624 / DSM 980 / FGSC 10383;
RX PubMed=18460219; DOI=10.1186/gb-2008-9-5-r77;
RA Espagne E., Lespinet O., Malagnac F., Da Silva C., Jaillon O., Porcel B.M.,
RA Couloux A., Aury J.-M., Segurens B., Poulain J., Anthouard V.,
RA Grossetete S., Khalili H., Coppin E., Dequard-Chablat M., Picard M.,
RA Contamine V., Arnaise S., Bourdais A., Berteaux-Lecellier V., Gautheret D.,
RA de Vries R.P., Battaglia E., Coutinho P.M., Danchin E.G.J., Henrissat B.,
RA El Khoury R., Sainsard-Chanet A., Boivin A., Pinan-Lucarre B., Sellem C.H.,
RA Debuchy R., Wincker P., Weissenbach J., Silar P.;
RT "The genome sequence of the model ascomycete fungus Podospora anserina.";
RL Genome Biol. 9:R77.1-R77.22(2008).
RN [2] {ECO:0000312|Proteomes:UP000001197}
RP GENOME REANNOTATION.
RC STRAIN=S / ATCC MYA-4624 / DSM 980 / FGSC 10383
RC {ECO:0000312|Proteomes:UP000001197};
RX PubMed=24558260; DOI=10.1534/genetics.113.159988;
RA Grognet P., Bidard F., Kuchly C., Tong L.C.H., Coppin E., Benkhali J.A.,
RA Couloux A., Wincker P., Debuchy R., Silar P.;
RT "Maintaining two mating types: Structure of the mating type locus and its
RT role in heterokaryosis in Podospora anserina.";
RL Genetics 197:421-432(2014).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP 707-ASP-GLU-708.
RX PubMed=24830502; DOI=10.1371/journal.pgen.1004387;
RA Grognet P., Lalucque H., Malagnac F., Silar P.;
RT "Genes that bias mendelian segregation.";
RL PLoS Genet. 10:E1004387-E1004387(2014).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=31347500; DOI=10.7554/elife.46454;
RA Vogan A.A., Ament-Velasquez S.L., Granger-Farbos A., Svedberg J.,
RA Bastiaans E., Debets A.J., Coustou V., Yvanne H., Clave C., Saupe S.J.,
RA Johannesson H.;
RT "Combinations of Spok genes create multiple meiotic drivers in Podospora.";
RL Elife 8:e46454-e46454(2019).
CC -!- FUNCTION: Promotes unequal transmission of alleles from the parental
CC zygote to progeny spores by acting as poison/antidote system, leading
CC to poisoning of progeny that do not inherit the allele.
CC {ECO:0000269|PubMed:24830502, ECO:0000269|PubMed:31347500}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24830502}. Nucleus
CC {ECO:0000269|PubMed:24830502}.
CC -!- DISRUPTION PHENOTYPE: Sensitises spores to SPOK2 encoded poison.
CC {ECO:0000269|PubMed:24830502}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAP62126.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; CU633457; CAP62126.1; ALT_FRAME; Genomic_DNA.
DR EMBL; FO904940; CDP29201.1; -; Genomic_DNA.
DR RefSeq; XP_001904347.1; XM_001904312.1.
DR AlphaFoldDB; B2AFA8; -.
DR EnsemblFungi; CAP62126; CAP62126; PODANS_5_10.
DR GeneID; 6188196; -.
DR KEGG; pan:PODANSg1366; -.
DR VEuPathDB; FungiDB:PODANS_5_10; -.
DR eggNOG; ENOG502SJ0M; Eukaryota.
DR HOGENOM; CLU_010672_2_0_1; -.
DR OrthoDB; 825916at2759; -.
DR Proteomes; UP000001197; Chromosome 5.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0110134; P:meiotic drive; IDA:UniProtKB.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR SUPFAM; SSF56112; SSF56112; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Nucleus; Reference proteome; Toxin.
FT CHAIN 1..775
FT /note="Meiotic driver SPOK2"
FT /id="PRO_0000453036"
FT REGION 18..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 442..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 734..761
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 4..69
FT /evidence="ECO:0000255"
FT COMPBIAS 213..227
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 442..480
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..498
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 707..708
FT /note="DE->AA: Abolishes antidote activity."
FT /evidence="ECO:0000269|PubMed:24830502"
SQ SEQUENCE 775 AA; 87293 MW; 51F4111585ACFA11 CRC64;
MSDKDRIAQL LRELEEAKAR EEEAKARAEE AKARAEEAKA RAEEAKAREE EAKAREAQER
CERERLQLEH RNTTFLEYLH NCHRHLYNAL RLTNTSRYST GYTKVVGKYY PKRLRPWTYF
ANVLHPHYFD LVQSICGQRQ LFEPANTTKS LGTIISDHLA GNEKAIDRFE VDAVERPVQG
ILKVLATHKE AGKASICPEF RFSANLRELT QKDDGSSGAD DNTSDGSLER RQQAGPNKRP
TSKRKYICSN RQPDGVGIRM QPGGGQTHAF IYDYKAAHKV AIEYIRSATA KEHLFHEVVA
RINDDKLSRD EEVQRREQAE ALIAMALTQV FDYMITYGVS YGYVAAGRCL LLLYVDRDDW
QTLYCHPCLP ADDVGEPTND WTDRLSHTAV AQLISFCLSS FQSEALEGQS LETALSIAKA
TLKTWSESYA DVAYLGLEPA ELSSAPSSQN TDISEYTSKA EPTGRNVALR SQSSCKPAAV
LPQSNEHDDE EDHSEPGASR LRLAANKRKR GPSSGGEDGD IAMADSGPTK QYCTQACLLG
LKRGKDLDEK CPNVSLHRFD GSSRHPVNAH RFTDMVEQQL LLSPYKGCRM VDFWGKRGAM
GWLFKLELLP YGYTFVGKGT LEDRLSRLEH EGRVYAQLDH LQGDVVPVYL GLVRLDRGYI
LPGLEFVVHM MLMSWAGQTP SASMDDAETL KRESLTAIWN EGVVHGDENR ANYLWNAERG
RIMIIDFDRA HLSPPPKPRA VSRLSKPKRK RGDSEADAQL FGPLEINRSK HRIRT
