ID   SPOK3_PODAS             Reviewed;         745 AA.
AC   A0A516F180;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Meiotic driver SPOK3 {ECO:0000305};
DE   AltName: Full=Spore killer 3 {ECO:0000303|PubMed:31347500};
GN   Name=SPOK3 {ECO:0000303|PubMed:31347500};
OS   Podospora anserina (Pleurage anserina).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Podosporaceae; Podospora.
OX   NCBI_TaxID=2587412 {ECO:0000312|EMBL:QDO72523.1};
RN   [1] {ECO:0000312|EMBL:QDO72523.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND MUTAGENESIS OF LYS-240;
RP   493-CYS--CYS-497; CYS-511 AND ASP-667.
RC   STRAIN=Wa87 {ECO:0000312|EMBL:QDO72523.1};
RX   PubMed=31347500; DOI=10.7554/elife.46454;
RA   Vogan A.A., Ament-Velasquez S.L., Granger-Farbos A., Svedberg J.,
RA   Bastiaans E., Debets A.J., Coustou V., Yvanne H., Clave C., Saupe S.J.,
RA   Johannesson H.;
RT   "Combinations of Spok genes create multiple meiotic drivers in Podospora.";
RL   Elife 8:e46454-e46454(2019).
CC   -!- FUNCTION: Promotes unequal transmission of alleles from the parental
CC       zygote to progeny spores by acting as poison/antidote system, leading
CC       to poisoning of progeny that do not inherit the allele.
CC       {ECO:0000269|PubMed:31347500}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2AFA8}. Nucleus
CC       {ECO:0000250|UniProtKB:B2AFA8}.
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DR   EMBL; MK521588; QDO72523.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A516F180; -.
DR   VEuPathDB; FungiDB:PODANS_5_10; -.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0110134; P:meiotic drive; IDA:UniProtKB.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Nucleus; Toxin.
FT   CHAIN           1..745
FT                   /note="Meiotic driver SPOK3"
FT                   /id="PRO_0000453037"
FT   REGION          173..222
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          214..325
FT                   /note="Required for antidote activity"
FT                   /evidence="ECO:0000269|PubMed:31347500"
FT   REGION          407..487
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          491..745
FT                   /note="Required for poison activity"
FT                   /evidence="ECO:0000269|PubMed:31347500"
FT   COILED          4..34
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        178..198
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        199..214
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        407..423
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        445..476
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         240
FT                   /note="K->A: Abolishes poison activity while retaining
FT                   antidote activity."
FT                   /evidence="ECO:0000269|PubMed:31347500"
FT   MUTAGEN         493..497
FT                   /note="CTQAC->ATQAA: Appears to retain poison activity
FT                   while abolishing antidote activity."
FT                   /evidence="ECO:0000269|PubMed:31347500"
FT   MUTAGEN         511
FT                   /note="C->A,S: Appears to retain spore killing activity
FT                   while abolishing antidote activity."
FT                   /evidence="ECO:0000269|PubMed:31347500"
FT   MUTAGEN         667
FT                   /note="D->A: Appears to retain spore killing activity while
FT                   abolishing antidote activity."
FT                   /evidence="ECO:0000269|PubMed:31347500"
SQ   SEQUENCE   745 AA;  84180 MW;  8C59A09E11666684 CRC64;
     MSDKDRITQL LRELEEAKAR EAQERCEKER LQLEHRKTTF LEYLRNCHRH LYNALRLTDT
     SRSSTGYTKV VGKYYPKRLR PWTNFTNVLH PHYFNLVQQI CGQRQLFESA STTKNLGTII
     SDHLAGNEKA IDRFEVDAVE RPVQGILKVL ATHEEAGKAS ICPEFRFSAN LRELTQKDDG
     SSGADDNTSD RSLERRQQAG PNKRPTSKSK YICSNRQPDG VGIRMQPDGG QTQAFIYDYK
     AAHKVAIEHV RSATAKEHLF HEVVARINDD KLSRDEEVQH REQAEAFIAM ALTQVFDYMI
     TYGVSYGYVA AGRCLLLLYV DRDDWQTLYC HPCLPADDVG EPTNDWTDRL SHTAVAQLVS
     FCLSSFQSEA LEGQSLETAL SVAKATLKTW SESYADVAYL GLEPAELSSA PSSQNTDISE
     YTSKAKPTGR KVTLRSCKPA AVLPQGNEHD EHDEEDHSEP GASRLAKRER GPSSGGKDDG
     RSMVDPQPTR QYCTQACLLG LKRGKDLDEN CPNVSLHRFD GSSRHPVNAH SFTDMVEQQL
     LLSPYKGCRM VDFWGKRGAM GWLFKLELLP YGYTFVGKGT VEDRLNRLEH EGRVYARLDH
     LQGDVVPVHL GLVRLDRGYI LPGLEFAVYM MLMSWAGQTP SANMDDAETL KSESLTAIWS
     EGVDHGDNNR ANYLWNAERC RIMIIDFDRA HLFPPLKARA VSRLSKPKRK RETLNSYLTE
     ITGREARHVF KQMDRSPMAT PSHGG