ID   SPOK4_PODAS             Reviewed;         758 AA.
AC   A0A516F183;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Meiotic driver SPOK4 {ECO:0000305};
DE   AltName: Full=Spore killer 4 {ECO:0000303|PubMed:31347500};
GN   Name=SPOK4 {ECO:0000303|PubMed:31347500};
OS   Podospora anserina (Pleurage anserina).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Podosporaceae; Podospora.
OX   NCBI_TaxID=2587412 {ECO:0000312|EMBL:QDO72524.1};
RN   [1] {ECO:0000312|EMBL:QDO72524.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=Wa87 {ECO:0000312|EMBL:QDO72524.1};
RX   PubMed=31347500; DOI=10.7554/elife.46454;
RA   Vogan A.A., Ament-Velasquez S.L., Granger-Farbos A., Svedberg J.,
RA   Bastiaans E., Debets A.J., Coustou V., Yvanne H., Clave C., Saupe S.J.,
RA   Johannesson H.;
RT   "Combinations of Spok genes create multiple meiotic drivers in Podospora.";
RL   Elife 8:e46454-e46454(2019).
CC   -!- FUNCTION: Promotes unequal transmission of alleles from the parental
CC       zygote to progeny spores by acting as poison/antidote system, leading
CC       to poisoning of progeny that do not inherit the allele
CC       (PubMed:31347500). Can suppress meiotic drive by the P.comata SPOK1
CC       protein (PubMed:31347500). {ECO:0000269|PubMed:31347500}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2AFA8}. Nucleus
CC       {ECO:0000250|UniProtKB:B2AFA8}.
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DR   EMBL; MK521589; QDO72524.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A516F183; -.
DR   VEuPathDB; FungiDB:PODANS_5_10; -.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0110134; P:meiotic drive; IDA:UniProtKB.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Nucleus; Toxin.
FT   CHAIN           1..758
FT                   /note="Meiotic driver SPOK4"
FT                   /id="PRO_0000453038"
FT   REGION          180..230
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          414..499
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          4..41
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        180..199
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        414..432
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        455..471
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   758 AA;  85956 MW;  EAFB83F5B8D6B963 CRC64;
     MSDKDRITQL LRKLEEAKAR EEEAKAREAQ ERCEKERLQL EHRKTTFLEY LRNCHRHLYN
     ALRLTDTSRS STGYTKVVGK YYPKRLRPWT NFTNVLHPRY FDLVQKICGQ RQLFEPASTT
     KNLGTIISDH LAGNEKAIDR FEVDAVERPV QGILKVLATH EEAGKAYRCP EFRFSANLRE
     LTQEDDRSSG ADDNTSDGSL ERRQQAGPNK RPTSKRKYIC SNRQPDGVGI RMQPGGGQTQ
     AFIYDYKAAH KVAIEHVRSA TAKEHLFHEV VARINDDKLS RDEEVQRREQ AEAFIAMALT
     QVFDYMITYG VSYGYVAAGR CLLLLYVDRD DWQTLYCHPC LPADDVGEPT NDWTDRLSHT
     AVAQLVSFCL SSFQSEALEG QSLETALSVA KATLKTWSES YADVAYLGLE PPELSSAASS
     QNTENSEYTS EAKPTGRKVA LRSQSSCKPA AVLPQGNEHD EHDEDHSEPG ASRSRLAANK
     RKRGPSSGGE DEDIAMADPE PTRQYCTQAC LLGLKRGKDL DENCPNVSLH RFDGSSRHPV
     NAHRFTDMVK QQLLLSPYKG CRMVDFWDKR GAMGWLFKLE LFPYGYTFVG KGTLEDRLSR
     LEHEGRVYAR LDHLQGDVVP VHLGLVRLDR GYILPGLEFV VYMMLMSWAG QTPSASMADA
     ETLKRESLTA IWSEGVDHGD DNRANYLWNA ERCRIMIIDF DRAHLFPPLK ARAVSRLSKP
     KRKRETPNSY LTEITGREAR YVFKQMDRSP RATPSHGG