ID   SPOLA_DANRE             Reviewed;         392 AA.
AC   Q5BL35;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Speckle-type POZ protein-like A;
DE   AltName: Full=HIB homolog 2;
GN   Name=spopla; Synonyms=spopl; ORFNames=zgc:101524;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=SJD;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=16740475; DOI=10.1016/j.devcel.2006.05.004;
RA   Zhang Q., Zhang L., Wang B., Ou C.-Y., Chien C.-T., Jiang J.;
RT   "A hedgehog-induced BTB protein modulates hedgehog signaling by degrading
RT   Ci/Gli transcription factor.";
RL   Dev. Cell 10:719-729(2006).
CC   -!- FUNCTION: Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin-protein ligase complex that mediates the ubiquitination and
CC       subsequent proteasomal degradation of target proteins, but with
CC       relatively low efficiency. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Homodimer. Heterodimer with SPOP. Component of cullin-RING-
CC       based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes
CC       containing homodimeric SPOPL or the heterodimer formed by SPOP and
CC       SPOPL (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Tdpoz family. {ECO:0000305}.
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DR   EMBL; BC090815; AAH90815.1; -; mRNA.
DR   RefSeq; NP_001013465.1; NM_001013447.1.
DR   AlphaFoldDB; Q5BL35; -.
DR   SMR; Q5BL35; -.
DR   STRING; 7955.ENSDARP00000110922; -.
DR   PaxDb; Q5BL35; -.
DR   Ensembl; ENSDART00000011922; ENSDARP00000026361; ENSDARG00000010563.
DR   Ensembl; ENSDART00000162818; ENSDARP00000134710; ENSDARG00000010563.
DR   GeneID; 541318; -.
DR   KEGG; dre:541318; -.
DR   CTD; 541318; -.
DR   ZFIN; ZDB-GENE-050320-3; spopla.
DR   eggNOG; KOG1987; Eukaryota.
DR   GeneTree; ENSGT00940000155953; -.
DR   HOGENOM; CLU_004253_2_0_1; -.
DR   InParanoid; Q5BL35; -.
DR   OMA; GAVMQSD; -.
DR   OrthoDB; 864323at2759; -.
DR   PhylomeDB; Q5BL35; -.
DR   Reactome; R-DRE-5632684; Hedgehog 'on' state.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q5BL35; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 9.
DR   Bgee; ENSDARG00000010563; Expressed in blastula and 21 other tissues.
DR   ExpressionAtlas; Q5BL35; baseline.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR   GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0030162; P:regulation of proteolysis; IBA:GO_Central.
DR   Gene3D; 2.60.210.10; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR002083; MATH/TRAF_dom.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   InterPro; IPR008974; TRAF-like.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF00917; MATH; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00061; MATH; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
DR   PROSITE; PS50144; MATH; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..392
FT                   /note="Speckle-type POZ protein-like A"
FT                   /id="PRO_0000274590"
FT   DOMAIN          31..161
FT                   /note="MATH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00129"
FT   DOMAIN          200..267
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
SQ   SEQUENCE   392 AA;  44233 MW;  963CE7E2345FA185 CRC64;
     MSGVPTPPPP GEMSSGPVAE SWCYTQVKVV KFSYMWTINN FSFCREEMGE VLKSSTFSSG
     PNDKMKWCLR VNPKGLDDES KDYLSLYLLL VSCPKSEVRA KFKFSLLNAK REETKAMESQ
     RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQSNMNMLK
     VPECQLSDDL GNLWECSRFT DCSLYVGGQE FKAHKSILAA RSPVFNAMFE HEMEESKKNR
     VDISDVEPEV FKEMMGFIYT GKAPNLEKMA DSLLAAADKY ALERLKVMCE EALCNSLSVE
     NVADTLILAD LHSAEQLKAQ AIDFINRCSV LRQLGCKDGK NWNSNHATDI METAGWKSMI
     QSHPHLVAEA FRALASAQCP HFGLPRKRLK QS