SPP1_MAIZE
ID SPP1_MAIZE Reviewed; 423 AA.
AC Q9FQ11;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Sucrose-phosphatase 1;
DE Short=ZmSPP1;
DE EC=3.1.3.24;
GN Name=SPP1;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=11050182; DOI=10.1073/pnas.230430197;
RA Lunn J.E., Ashton A.R., Hatch M.D., Heldt H.W.;
RT "Purification, molecular cloning, and sequence analysis of sucrose-6F-
RT phosphate phosphohydrolase from plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12914-12919(2000).
RN [2]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12559580; DOI=10.1016/s0378-1119(02)01177-0;
RA Lunn J.E.;
RT "Sucrose-phosphatase gene families in plants.";
RL Gene 303:187-196(2003).
CC -!- FUNCTION: Catalyzes the final step of sucrose synthesis. Inactive with
CC fructose 6-phosphate as substrate.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + sucrose 6(F)-phosphate = phosphate + sucrose;
CC Xref=Rhea:RHEA:19289, ChEBI:CHEBI:15377, ChEBI:CHEBI:17992,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57723; EC=3.1.3.24;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC Vmax=370 nmol/min/mg enzyme {ECO:0000269|PubMed:11050182};
CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sucrose phosphatase family. {ECO:0000305}.
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DR EMBL; AF283564; AAG31074.1; -; mRNA.
DR RefSeq; NP_001105006.1; NM_001111536.2.
DR AlphaFoldDB; Q9FQ11; -.
DR SMR; Q9FQ11; -.
DR STRING; 4577.GRMZM2G055489_P02; -.
DR PaxDb; Q9FQ11; -.
DR PRIDE; Q9FQ11; -.
DR GeneID; 541868; -.
DR MaizeGDB; 403253; -.
DR eggNOG; ENOG502QTVT; Eukaryota.
DR BRENDA; 3.1.3.24; 6752.
DR UniPathway; UPA00371; UER00546.
DR Proteomes; UP000007305; Unplaced.
DR ExpressionAtlas; Q9FQ11; baseline and differential.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0050307; F:sucrose-phosphate phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006379; HAD-SF_hydro_IIB.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR013679; SPP_C.
DR InterPro; IPR006380; SPP_N.
DR InterPro; IPR012847; Sucrose_phosphatase_pln/cyn.
DR Pfam; PF05116; S6PP; 1.
DR Pfam; PF08472; S6PP_C; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR TIGRFAMs; TIGR01485; SPP_plant-cyano; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Magnesium; Reference proteome.
FT CHAIN 1..423
FT /note="Sucrose-phosphatase 1"
FT /id="PRO_0000350620"
SQ SEQUENCE 423 AA; 47214 MW; 70F5BC24636F5278 CRC64;
MDKLSGSARL MIVSDLDHTM VDHHDEENLS LLRFGALWES VYCEDSLLVF STGRSPTLYK
ELRKEKPMLT PDITIMSVGT EITYGEAMVP DDGWEEYLNN KWDRNIVVAE TVSFSELKLQ
PETEQRPHKV SFFVDKKNAQ EVIKSVAERL DKCGLDAKII YSGGQDLDIL PQGAGKGQAL
AYLLEKLSSC GKPPNNTLVC GDSGNDAELF SIPGVHGVMV SNAQEELLQW YTENAKDNPK
IIHSNERCAA GIIQAIGHFK LGPNISPRDL QFPYAKEASF KPTDAVVKFY VLYEKWRRAE
VPKSDSVIKY FKNITHANGV TIHPAGLELS LHASIDALGS CYGDKQGRKY RAWVDRLFIT
QTGSDSWVGR FDLWESEGDV RVCSLSSLAL ILKAESPEGF VLTHIQKTWL NGYSSGVEQA
FKL
