SPP1_TOBAC
ID SPP1_TOBAC Reviewed; 425 AA.
AC Q5IH14;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 25-MAY-2022, entry version 58.
DE RecName: Full=Sucrose-phosphatase 1;
DE Short=NtSPP1;
DE EC=3.1.3.24;
GN Name=SPP1;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ACTIVITY REGULATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Samsun NN;
RX PubMed=15657716; DOI=10.1007/s00425-004-1458-4;
RA Chen S., Hajirezaei M., Peisker M., Tschiersch H., Sonnewald U.,
RA Boernke F.;
RT "Decreased sucrose-6-phosphate phosphatase level in transgenic tobacco
RT inhibits photosynthesis, alters carbohydrate partitioning, and reduces
RT growth.";
RL Planta 221:479-492(2005).
CC -!- FUNCTION: Catalyzes the final step of sucrose synthesis.
CC {ECO:0000269|PubMed:15657716}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + sucrose 6(F)-phosphate = phosphate + sucrose;
CC Xref=Rhea:RHEA:19289, ChEBI:CHEBI:15377, ChEBI:CHEBI:17992,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57723; EC=3.1.3.24;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- ACTIVITY REGULATION: Inhibited by EDTA. {ECO:0000269|PubMed:15657716}.
CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Plants show chlorosis, inhibition of
CC photosynthesis, reduced growth rate and altered photosynthetic carbon
CC partitioning in favor of starch. {ECO:0000269|PubMed:15657716}.
CC -!- SIMILARITY: Belongs to the sucrose phosphatase family. {ECO:0000305}.
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DR EMBL; AY729655; AAW32902.1; -; mRNA.
DR RefSeq; NP_001311887.1; NM_001324958.1.
DR AlphaFoldDB; Q5IH14; -.
DR SMR; Q5IH14; -.
DR STRING; 4097.Q5IH14; -.
DR GeneID; 107767908; -.
DR KEGG; nta:107767908; -.
DR UniPathway; UPA00371; UER00546.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0050307; F:sucrose-phosphate phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006379; HAD-SF_hydro_IIB.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR013679; SPP_C.
DR InterPro; IPR006380; SPP_N.
DR InterPro; IPR012847; Sucrose_phosphatase_pln/cyn.
DR Pfam; PF05116; S6PP; 1.
DR Pfam; PF08472; S6PP_C; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR TIGRFAMs; TIGR01485; SPP_plant-cyano; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Reference proteome.
FT CHAIN 1..425
FT /note="Sucrose-phosphatase 1"
FT /id="PRO_0000350622"
SQ SEQUENCE 425 AA; 47878 MW; E017AA9302716F0D CRC64;
MDQLTSAARL MIVSDLDHTM VDHHDPENLS LLRFNALWEA NYRENSLLVF STGRSPTLYK
ELRKEKPMLT PDITIMSVGT EITYGNSMEP DDGWEAFLND KWDRKIVTEE TSKFPELTLQ
SETEQRPHKV SFYVQKDKAQ DITGTLSKRL EERGLDVKII YSGGMDLDIL PQGAGKGRAL
AYLLKKLKSE GKLPNNTLAC GDSGNDAELF SIPDVYGVMV ANAQEELLQW RAANAKDSPK
VIHATERCAA GIIQAIGHFN LGPNTSPRDV TDMSDCKMEN FVPAYEVVKF YLFFEKWRRG
EIENSDLHLS NLKAVCRPSG TFVHPSGVEK YLEDCINTLR TCHGDKQGKQ FRIWVDLVLP
TQVGSDSWLV SFKKWELCGE ERQCCITTVL LSSKNVTVAD GLTWTHVHQT WLQGAAASDS
ASWFF
