SPP24_MOUSE
ID SPP24_MOUSE Reviewed; 203 AA.
AC Q8K1I3; Q9DCG1;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2002, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Secreted phosphoprotein 24;
DE Short=Spp-24;
DE AltName: Full=Secreted phosphoprotein 2;
DE Flags: Precursor;
GN Name=Spp2; Synonyms=Spp24;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162 AND SER-165, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Could coordinate an aspect of bone turnover. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- PTM: Phosphorylation sites are present in the extracellular medium.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SPP2 family. {ECO:0000305}.
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DR EMBL; AK002814; BAB22379.1; -; mRNA.
DR EMBL; BC027494; AAH27494.1; -; mRNA.
DR CCDS; CCDS15145.1; -.
DR RefSeq; NP_083545.1; NM_029269.3.
DR AlphaFoldDB; Q8K1I3; -.
DR SMR; Q8K1I3; -.
DR STRING; 10090.ENSMUSP00000027518; -.
DR iPTMnet; Q8K1I3; -.
DR PhosphoSitePlus; Q8K1I3; -.
DR EPD; Q8K1I3; -.
DR MaxQB; Q8K1I3; -.
DR PaxDb; Q8K1I3; -.
DR PeptideAtlas; Q8K1I3; -.
DR PRIDE; Q8K1I3; -.
DR ProteomicsDB; 257325; -.
DR Antibodypedia; 34459; 45 antibodies from 7 providers.
DR DNASU; 75396; -.
DR Ensembl; ENSMUST00000027518; ENSMUSP00000027518; ENSMUSG00000026295.
DR GeneID; 75396; -.
DR KEGG; mmu:75396; -.
DR UCSC; uc007byq.1; mouse.
DR CTD; 6694; -.
DR MGI; MGI:1922646; Spp2.
DR VEuPathDB; HostDB:ENSMUSG00000026295; -.
DR eggNOG; ENOG502S7TB; Eukaryota.
DR GeneTree; ENSGT00390000009001; -.
DR HOGENOM; CLU_115216_0_0_1; -.
DR InParanoid; Q8K1I3; -.
DR OMA; NHQHRAR; -.
DR OrthoDB; 1281655at2759; -.
DR PhylomeDB; Q8K1I3; -.
DR TreeFam; TF335972; -.
DR Reactome; R-MMU-114608; Platelet degranulation.
DR Reactome; R-MMU-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-MMU-8957275; Post-translational protein phosphorylation.
DR BioGRID-ORCS; 75396; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Spp2; mouse.
DR PRO; PR:Q8K1I3; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8K1I3; protein.
DR Bgee; ENSMUSG00000026295; Expressed in right kidney and 58 other tissues.
DR ExpressionAtlas; Q8K1I3; baseline and differential.
DR Genevisible; Q8K1I3; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0046849; P:bone remodeling; IEA:InterPro.
DR GO; GO:0065003; P:protein-containing complex assembly; ISO:MGI.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR010892; Spp-24.
DR PANTHER; PTHR15444; PTHR15444; 1.
DR Pfam; PF07448; Spp-24; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Phosphoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000250"
FT CHAIN 24..203
FT /note="Secreted phosphoprotein 24"
FT /id="PRO_0000022404"
FT MOD_RES 90
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13103"
FT MOD_RES 137
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q62740"
FT MOD_RES 138
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q62740"
FT MOD_RES 162
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 165
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 174
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q62740"
FT DISULFID 86..96
FT /evidence="ECO:0000250"
FT DISULFID 109..127
FT /evidence="ECO:0000250"
FT CONFLICT 109
FT /note="C -> F (in Ref. 2; AAH27494)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 203 AA; 23136 MW; B583D059A2C8F65D CRC64;
MEQAMLKTLA LLVLGMHYWC ATGFPVYDYD PSSLQEALSA SVAKVNSQSL SPYLFRATRS
SLKRVNVLDE DTLVMNLEFS VQETTCLRDS GDPSTCAFQR GYSVPTAACR STVQMSKGQV
KDVWAHCRWA SSSESNSSEE MMFGDMARSH RRRNDYLLGF LSDESRSEQF RDRSLEIMRR
GQPPAHRRFL NLHRRARVNS GFE
