SPP2_ARATH
ID SPP2_ARATH Reviewed; 422 AA.
AC Q9SJ66; Q5XVC8; Q940K7; Q9FUW2;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable sucrose-phosphatase 2;
DE Short=AtSPP2;
DE EC=3.1.3.24;
GN Name=SPP2; Synonyms=SPP1; OrderedLocusNames=At2g35840; ORFNames=F11F19.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 3-422.
RC STRAIN=cv. Columbia;
RX PubMed=11050182; DOI=10.1073/pnas.230430197;
RA Lunn J.E., Ashton A.R., Hatch M.D., Heldt H.W.;
RT "Purification, molecular cloning, and sequence analysis of sucrose-6F-
RT phosphate phosphohydrolase from plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12914-12919(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 165-422.
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12559580; DOI=10.1016/s0378-1119(02)01177-0;
RA Lunn J.E.;
RT "Sucrose-phosphatase gene families in plants.";
RL Gene 303:187-196(2003).
CC -!- FUNCTION: Catalyzes the final step of sucrose synthesis. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + sucrose 6(F)-phosphate = phosphate + sucrose;
CC Xref=Rhea:RHEA:19289, ChEBI:CHEBI:15377, ChEBI:CHEBI:17992,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57723; EC=3.1.3.24;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sucrose phosphatase family. {ECO:0000305}.
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DR EMBL; AC007017; AAD21473.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC09168.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09169.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09170.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM63145.1; -; Genomic_DNA.
DR EMBL; AY054474; AAK96665.1; -; mRNA.
DR EMBL; AY114634; AAM47953.1; -; mRNA.
DR EMBL; AF283565; AAG31075.1; -; mRNA.
DR EMBL; AY735594; AAU44464.1; -; mRNA.
DR EMBL; AY954816; AAX55142.1; -; mRNA.
DR PIR; F84773; F84773.
DR RefSeq; NP_001189687.1; NM_001202758.1.
DR RefSeq; NP_001325253.1; NM_001336575.1.
DR RefSeq; NP_565828.1; NM_129143.4.
DR RefSeq; NP_973609.1; NM_201880.2.
DR AlphaFoldDB; Q9SJ66; -.
DR SMR; Q9SJ66; -.
DR BioGRID; 3501; 2.
DR STRING; 3702.AT2G35840.3; -.
DR PaxDb; Q9SJ66; -.
DR PRIDE; Q9SJ66; -.
DR ProteomicsDB; 234095; -.
DR DNASU; 818157; -.
DR EnsemblPlants; AT2G35840.1; AT2G35840.1; AT2G35840.
DR EnsemblPlants; AT2G35840.2; AT2G35840.2; AT2G35840.
DR EnsemblPlants; AT2G35840.3; AT2G35840.3; AT2G35840.
DR EnsemblPlants; AT2G35840.4; AT2G35840.4; AT2G35840.
DR GeneID; 818157; -.
DR Gramene; AT2G35840.1; AT2G35840.1; AT2G35840.
DR Gramene; AT2G35840.2; AT2G35840.2; AT2G35840.
DR Gramene; AT2G35840.3; AT2G35840.3; AT2G35840.
DR Gramene; AT2G35840.4; AT2G35840.4; AT2G35840.
DR KEGG; ath:AT2G35840; -.
DR Araport; AT2G35840; -.
DR TAIR; locus:2039295; AT2G35840.
DR eggNOG; ENOG502S8I4; Eukaryota.
DR HOGENOM; CLU_030534_1_0_1; -.
DR InParanoid; Q9SJ66; -.
DR OMA; FNLWELE; -.
DR OrthoDB; 612145at2759; -.
DR PhylomeDB; Q9SJ66; -.
DR BioCyc; ARA:AT2G35840-MON; -.
DR BRENDA; 3.1.3.24; 399.
DR UniPathway; UPA00371; UER00546.
DR PRO; PR:Q9SJ66; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJ66; baseline and differential.
DR Genevisible; Q9SJ66; AT.
DR GO; GO:0005737; C:cytoplasm; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0050307; F:sucrose-phosphate phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006379; HAD-SF_hydro_IIB.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR013679; SPP_C.
DR InterPro; IPR006380; SPP_N.
DR InterPro; IPR012847; Sucrose_phosphatase_pln/cyn.
DR Pfam; PF05116; S6PP; 1.
DR Pfam; PF08472; S6PP_C; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR TIGRFAMs; TIGR01485; SPP_plant-cyano; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Reference proteome.
FT CHAIN 1..422
FT /note="Probable sucrose-phosphatase 2"
FT /id="PRO_0000350614"
SQ SEQUENCE 422 AA; 47855 MW; 6CC4B0D5A1AF4BCF CRC64;
MERLTSPPRL MIVSDLDHTM VDHHDPENLS LLRFNSLWEH AYRHDSLLVF STGRSPTLYK
ELRKEKPLLT PDITIMSVGT EITYGNSMVP DHGWVEALNN KWDLGIVKQE ASNFPELKLQ
AETEQRPHKV SFYVEKSKAQ EVTKELSQRF LKRGLDVKII YSGGMDLDIL PQGAGKGQAL
AYLLKKLKTE GKLPVNTLAC GDSGNDAELF SIPDVYGVMV SNAQEELLKW HAENAKDNPK
VIHAKERCAG GIIQAIGHFK LGPNLSPRDV SDFLEIKVEN VNPGHEVVKF FLFYERWRRG
EVENSEAYTA SLKASVHPGG VFVHPSGTEK SLRDTIDELR KYHGDKQGKK FRVWADQVLA
TDTTPGTWIV KLDKWEQDGD ERRCCTTTVK FTSKEGEGLV WEHVQQTWSK ETMVKDDSSW
II
