ID   SPP2_MAIZE              Reviewed;         437 AA.
AC   Q84ZX8;
DT   23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Sucrose-phosphatase 2;
DE            Short=ZmSPP2;
DE            EC=3.1.3.24;
GN   Name=SPP2;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, AND
RP   NOMENCLATURE.
RC   TISSUE=Seedling;
RX   PubMed=12559580; DOI=10.1016/s0378-1119(02)01177-0;
RA   Lunn J.E.;
RT   "Sucrose-phosphatase gene families in plants.";
RL   Gene 303:187-196(2003).
CC   -!- FUNCTION: Catalyzes the final step of sucrose synthesis. Inactive with
CC       fructose 6-phosphate as substrate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + sucrose 6(F)-phosphate = phosphate + sucrose;
CC         Xref=Rhea:RHEA:19289, ChEBI:CHEBI:15377, ChEBI:CHEBI:17992,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57723; EC=3.1.3.24;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         Vmax=182 nmol/min/mg enzyme {ECO:0000269|PubMed:12559580};
CC   -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC       fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sucrose phosphatase family. {ECO:0000305}.
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DR   EMBL; AF493561; AAO33158.1; -; mRNA.
DR   RefSeq; NP_001105652.1; NM_001112182.1.
DR   AlphaFoldDB; Q84ZX8; -.
DR   SMR; Q84ZX8; -.
DR   STRING; 4577.GRMZM2G097641_P02; -.
DR   PRIDE; Q84ZX8; -.
DR   EnsemblPlants; Zm00001eb417940_T001; Zm00001eb417940_P001; Zm00001eb417940.
DR   EnsemblPlants; Zm00001eb417940_T002; Zm00001eb417940_P002; Zm00001eb417940.
DR   EnsemblPlants; Zm00001eb417940_T004; Zm00001eb417940_P004; Zm00001eb417940.
DR   GeneID; 542661; -.
DR   Gramene; Zm00001eb417940_T001; Zm00001eb417940_P001; Zm00001eb417940.
DR   Gramene; Zm00001eb417940_T002; Zm00001eb417940_P002; Zm00001eb417940.
DR   Gramene; Zm00001eb417940_T004; Zm00001eb417940_P004; Zm00001eb417940.
DR   KEGG; zma:542661; -.
DR   eggNOG; ENOG502S2R4; Eukaryota.
DR   HOGENOM; CLU_030534_1_0_1; -.
DR   OrthoDB; 612145at2759; -.
DR   UniPathway; UPA00371; UER00546.
DR   Proteomes; UP000007305; Chromosome 10.
DR   ExpressionAtlas; Q84ZX8; baseline and differential.
DR   Genevisible; Q84ZX8; ZM.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0050307; F:sucrose-phosphate phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR006379; HAD-SF_hydro_IIB.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR013679; SPP_C.
DR   InterPro; IPR006380; SPP_N.
DR   InterPro; IPR012847; Sucrose_phosphatase_pln/cyn.
DR   Pfam; PF05116; S6PP; 1.
DR   Pfam; PF08472; S6PP_C; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR   TIGRFAMs; TIGR01485; SPP_plant-cyano; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Reference proteome.
FT   CHAIN           1..437
FT                   /note="Sucrose-phosphatase 2"
FT                   /id="PRO_0000350621"
SQ   SEQUENCE   437 AA;  48585 MW;  9F36BB70F0C658BE CRC64;
     MDRLDGSARL LLVSDLDQTM IDHDDRENLS LLRFEALWEA EFAQDSLLVF STGRTPVSYK
     GLRRDKPLVT PDVTIMSVGT VIAYGEEMVR DVGWEERLDS DWDRAVVVEE TARLPQLTRM
     QPERNQGPHK VSFFVDKQGA QEVMDYLPKK LERRGVDVKI VYSSGNALDV LPRGAGKGQA
     LVYLLKKLDS NGKPPKNTLV CGDSGNDAEL FGVPSVHGVM VCNAQEELVQ WYQENARDNP
     RIIQATERCA AGIMQAIGHL GLGPNVSARD LVGFPAYYPN NNKKGAAATA KPADVVVRFY
     VLYEKWRRGE LPSSNYFSSS SAMRYLKSIT HPNGTIIHPC GSERSLHASV DALLSSCYGD
     KKNFRVWVDR LVTSPIGTSS WLARFDSWEM EGGVRYCCRT TILLNIKPES PEGLELTHIH
     KTWVQGHSAG PGSALVF