ID   SPP2_ORYSJ              Reviewed;         423 AA.
AC   Q6YXW6; A3A323; B7E8F4;
DT   23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Sucrose-phosphatase 2;
DE            Short=OsSPP2;
DE            EC=3.1.3.24;
GN   Name=SPP2; OrderedLocusNames=Os02g0143100, LOC_Os02g05030;
GN   ORFNames=OsJ_005195, OSJNBa0064G16.2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   PROTEIN SEQUENCE OF 102-118; 261-276 AND 289-295, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND SUBUNIT.
RX   PubMed=11050182; DOI=10.1073/pnas.230430197;
RA   Lunn J.E., Ashton A.R., Hatch M.D., Heldt H.W.;
RT   "Purification, molecular cloning, and sequence analysis of sucrose-6F-
RT   phosphate phosphohydrolase from plants.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12914-12919(2000).
RN   [7]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12559580; DOI=10.1016/s0378-1119(02)01177-0;
RA   Lunn J.E.;
RT   "Sucrose-phosphatase gene families in plants.";
RL   Gene 303:187-196(2003).
CC   -!- FUNCTION: Catalyzes the final step of sucrose synthesis. Inactive with
CC       fructose 6-phosphate as substrate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + sucrose 6(F)-phosphate = phosphate + sucrose;
CC         Xref=Rhea:RHEA:19289, ChEBI:CHEBI:15377, ChEBI:CHEBI:17992,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57723; EC=3.1.3.24;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- ACTIVITY REGULATION: Inactivated by zinc.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=65 uM for sucrose 6(F)-phosphate {ECO:0000269|PubMed:11050182};
CC   -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC       fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:11050182}.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the sucrose phosphatase family. {ECO:0000305}.
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DR   EMBL; AP005649; BAD13232.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF07780.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS76941.1; -; Genomic_DNA.
DR   EMBL; CM000139; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK063330; BAG88651.1; -; mRNA.
DR   RefSeq; XP_015622612.1; XM_015767126.1.
DR   AlphaFoldDB; Q6YXW6; -.
DR   SMR; Q6YXW6; -.
DR   STRING; 4530.OS02T0143100-01; -.
DR   PaxDb; Q6YXW6; -.
DR   PRIDE; Q6YXW6; -.
DR   EnsemblPlants; Os02t0143100-01; Os02t0143100-01; Os02g0143100.
DR   GeneID; 4328274; -.
DR   Gramene; Os02t0143100-01; Os02t0143100-01; Os02g0143100.
DR   KEGG; osa:4328274; -.
DR   eggNOG; ENOG502QTVT; Eukaryota.
DR   HOGENOM; CLU_030534_1_0_1; -.
DR   InParanoid; Q6YXW6; -.
DR   OMA; FNLWELE; -.
DR   OrthoDB; 612145at2759; -.
DR   PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR   SABIO-RK; Q6YXW6; -.
DR   UniPathway; UPA00371; UER00546.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; Q6YXW6; OS.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0050307; F:sucrose-phosphate phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR006379; HAD-SF_hydro_IIB.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR013679; SPP_C.
DR   InterPro; IPR006380; SPP_N.
DR   InterPro; IPR012847; Sucrose_phosphatase_pln/cyn.
DR   Pfam; PF05116; S6PP; 1.
DR   Pfam; PF08472; S6PP_C; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR   TIGRFAMs; TIGR01485; SPP_plant-cyano; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Magnesium; Reference proteome.
FT   CHAIN           1..423
FT                   /note="Sucrose-phosphatase 2"
FT                   /id="PRO_0000350618"
SQ   SEQUENCE   423 AA;  47255 MW;  1F43204FFF6080B5 CRC64;
     MDKLNGSARL MIVSDLDHTM VDHHDEENLS LLRFGALWES VYCQDSLLVF STGRSPTLYM
     ELRKEKPMLT PDITIMSVGT EITYGEEMVP DDGWVEYLNN KWDRNIVVEE TANVSELKLQ
     VESEQRPHKV SFYVDKKSAQ EVIKSLSEKL EKRGLDVKII YSGGQDLDVL PQGAGKGQAL
     AYLLKKLSSC GKPPNNTLAC GDSGNDAELF SIPGVHGVMV SNAQEELLQW YSENAKDNPK
     IIHATERCAA GIIQAIGHFK LGPNVSPRDV DFPYVKENPV KPTDAVVKFY VLYEKWRRAE
     VPKSDSVTQY FKNITHANGV IIHPAGLECS LHASIDALGS CYGDKQGKKY RAWVDRLVVS
     QCGSEGWLVR FNLWELEGDV WSCCLTSLAL NAKPETPEGF VVTHIHKTWL KGYSSADEQS
     SKL