SPP2_TOBAC
ID SPP2_TOBAC Reviewed; 425 AA.
AC Q5IH13;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Sucrose-phosphatase 2;
DE Short=NtSPP2;
DE EC=3.1.3.24;
GN Name=SPP2;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ACTIVITY REGULATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Samsun NN;
RX PubMed=15657716; DOI=10.1007/s00425-004-1458-4;
RA Chen S., Hajirezaei M., Peisker M., Tschiersch H., Sonnewald U.,
RA Boernke F.;
RT "Decreased sucrose-6-phosphate phosphatase level in transgenic tobacco
RT inhibits photosynthesis, alters carbohydrate partitioning, and reduces
RT growth.";
RL Planta 221:479-492(2005).
CC -!- FUNCTION: Catalyzes the final step of sucrose synthesis.
CC {ECO:0000269|PubMed:15657716}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + sucrose 6(F)-phosphate = phosphate + sucrose;
CC Xref=Rhea:RHEA:19289, ChEBI:CHEBI:15377, ChEBI:CHEBI:17992,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57723; EC=3.1.3.24;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- ACTIVITY REGULATION: Inhibited by EDTA. {ECO:0000269|PubMed:15657716}.
CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Plants show chlorosis, inhibition of
CC photosynthesis, reduced growth rate and altered photosynthetic carbon
CC partitioning in favor of starch. {ECO:0000269|PubMed:15657716}.
CC -!- SIMILARITY: Belongs to the sucrose phosphatase family. {ECO:0000305}.
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DR EMBL; AY729656; AAW32903.1; -; mRNA.
DR RefSeq; NP_001312971.1; NM_001326042.1.
DR AlphaFoldDB; Q5IH13; -.
DR SMR; Q5IH13; -.
DR STRING; 4097.Q5IH13; -.
DR ProMEX; Q5IH13; -.
DR GeneID; 107819363; -.
DR KEGG; nta:107819363; -.
DR UniPathway; UPA00371; UER00546.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0050307; F:sucrose-phosphate phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006379; HAD-SF_hydro_IIB.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR013679; SPP_C.
DR InterPro; IPR006380; SPP_N.
DR InterPro; IPR012847; Sucrose_phosphatase_pln/cyn.
DR Pfam; PF05116; S6PP; 1.
DR Pfam; PF08472; S6PP_C; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR TIGRFAMs; TIGR01485; SPP_plant-cyano; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Reference proteome.
FT CHAIN 1..425
FT /note="Sucrose-phosphatase 2"
FT /id="PRO_0000350623"
SQ SEQUENCE 425 AA; 47784 MW; DBF4E0E0929E75F4 CRC64;
MDQLTSAARL MIVSDLDHTM VDHHDAENLS LLRFNALWEA NYRDNSLLVF STGRSPTLYK
ELRKEKPMLT PDITIMSVGT EITYGNSVVP DDGWEAFLNN KWDRKIVTEE TSKFPELTLQ
SETEQRPHKV SFYVQKDKAQ DIMKTLSKRF EERGLDVKII YSGGMDLDIL PQGAGKGQAL
AYLLKKLKSE GKLPNNTLAC GDSGNDAELF SIPDVYGVMV ANAQEELLQW HAANAKNNPK
VIHATERCAA GIIQAIGHSN LGPSTSPRDV MDLSDCKMEN FVPAYEVVKF YLFFEKWRRG
EIEHSEHYLS NLKAVCRPSG TFVHPSGVEK SLQECVTLFG TCHGDKQGKQ FRIWVDQVLP
VQVGSDSWLV SFKKWELSGE DRRCCITTVL LSSKNKTVAD GLTWTHVHQT WLNGAAASDS
ASWFF
