SPP3A_ARATH
ID SPP3A_ARATH Reviewed; 425 AA.
AC Q93WU4; Q9M379;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Probable sucrose-phosphatase 3a;
DE Short=AtSPP3a;
DE EC=3.1.3.24;
GN Name=SPP3A; Synonyms=SPP3; OrderedLocusNames=At3g54270;
GN ORFNames=F24B22.230;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 48-425.
RC STRAIN=cv. Columbia; TISSUE=Seed;
RX PubMed=11050182; DOI=10.1073/pnas.230430197;
RA Lunn J.E., Ashton A.R., Hatch M.D., Heldt H.W.;
RT "Purification, molecular cloning, and sequence analysis of sucrose-6F-
RT phosphate phosphohydrolase from plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12914-12919(2000).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12559580; DOI=10.1016/s0378-1119(02)01177-0;
RA Lunn J.E.;
RT "Sucrose-phosphatase gene families in plants.";
RL Gene 303:187-196(2003).
CC -!- FUNCTION: Catalyzes the final step of sucrose synthesis. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + sucrose 6(F)-phosphate = phosphate + sucrose;
CC Xref=Rhea:RHEA:19289, ChEBI:CHEBI:15377, ChEBI:CHEBI:17992,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57723; EC=3.1.3.24;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sucrose phosphatase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB71001.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132957; CAB71001.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79206.1; -; Genomic_DNA.
DR EMBL; AF434711; AAL30747.1; -; mRNA.
DR PIR; T47586; T47586.
DR RefSeq; NP_190995.2; NM_115287.4.
DR AlphaFoldDB; Q93WU4; -.
DR SMR; Q93WU4; -.
DR STRING; 3702.AT3G54270.1; -.
DR PaxDb; Q93WU4; -.
DR PRIDE; Q93WU4; -.
DR ProteomicsDB; 245201; -.
DR EnsemblPlants; AT3G54270.1; AT3G54270.1; AT3G54270.
DR GeneID; 824594; -.
DR Gramene; AT3G54270.1; AT3G54270.1; AT3G54270.
DR KEGG; ath:AT3G54270; -.
DR Araport; AT3G54270; -.
DR TAIR; locus:2080295; AT3G54270.
DR eggNOG; ENOG502S2R4; Eukaryota.
DR HOGENOM; CLU_030534_1_0_1; -.
DR InParanoid; Q93WU4; -.
DR OMA; ENFHVEV; -.
DR OrthoDB; 612145at2759; -.
DR PhylomeDB; Q93WU4; -.
DR BioCyc; ARA:AT3G54270-MON; -.
DR UniPathway; UPA00371; UER00546.
DR PRO; PR:Q93WU4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q93WU4; baseline and differential.
DR Genevisible; Q93WU4; AT.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0050307; F:sucrose-phosphate phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006379; HAD-SF_hydro_IIB.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR013679; SPP_C.
DR InterPro; IPR006380; SPP_N.
DR InterPro; IPR012847; Sucrose_phosphatase_pln/cyn.
DR Pfam; PF05116; S6PP; 1.
DR Pfam; PF08472; S6PP_C; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01484; HAD-SF-IIB; 1.
DR TIGRFAMs; TIGR01485; SPP_plant-cyano; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Magnesium; Reference proteome.
FT CHAIN 1..425
FT /note="Probable sucrose-phosphatase 3a"
FT /id="PRO_0000350615"
SQ SEQUENCE 425 AA; 48644 MW; 7E8EFC939E5E1A3F CRC64;
MDRLEGPPRL ILVADLDCTL VDHDDPENND LLRFNALWEA HYRHDSLLVY CTGRSFSSYS
SLRKKRPLLT PDIAVTSVGS EIVYGGGEST VSDVVWTARL DYKWNRDIVV EETLKFPKLE
PQPDKSQEEH KVSFFVGRED AVEIMKVLPG ILEERGVDVK LVYSNGYAFD VLPRGAGKQG
ALTYLLDKLD IEGKQPSNTL VCGDSGNDAE LFNISDVYGV MVSNSHEELL QWYEENAKDN
PKIFHASERC GAGMIEAIQR FNLGPNVSPR DVMDTENFHG ESLNPAHEVV QFYLFYERWR
CGEVEKSDKY LQNLKSLSSP LGIFVHPSGV EKPIHEWIDE MENLYGDGKE KKFRIWLDNV
TSSHISSDTW LAKFVKHELS EGKVRSCSTK VLLSYKEEKQ RLTWMHIHQS WLDESSSDDQ
EKWIF
