ID   SPPA_ENTFC              Reviewed;         344 AA.
AC   Q9X480;
DT   20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Putative signal peptide peptidase SppA;
DE            EC=3.4.21.-;
GN   Name=sppA;
OS   Enterococcus faecium (Streptococcus faecium).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1352;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DPC1146;
RX   PubMed=10103244; DOI=10.1128/aem.65.4.1506-1515.1999;
RA   O'Keeffe T., Hill C., Ross R.P.;
RT   "Characterization and heterologous expression of the genes encoding
RT   enterocin A production, immunity, and regulation in Enterococcus faecium
RT   DPC1146.";
RL   Appl. Environ. Microbiol. 65:1506-1515(1999).
CC   -!- FUNCTION: Digestion of the cleaved signal peptides. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S49 family. {ECO:0000305}.
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DR   EMBL; AF099088; AAD29142.1; -; Genomic_DNA.
DR   RefSeq; WP_002294607.1; NZ_WVTH01000009.1.
DR   AlphaFoldDB; Q9X480; -.
DR   SMR; Q9X480; -.
DR   GeneID; 66455280; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07023; S49_Sppa_N_C; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR004635; Pept_S49_SppA.
DR   InterPro; IPR002142; Peptidase_S49.
DR   Pfam; PF01343; Peptidase_S49; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   TIGRFAMs; TIGR00706; SppA_dom; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Protease; Serine protease;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..344
FT                   /note="Putative signal peptide peptidase SppA"
FT                   /id="PRO_0000171443"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        150
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        202
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   344 AA;  37543 MW;  B3D12FA2F6DCADE0 CRC64;
     MNKRRWIAVG VACGLLLLSI IVALIPGKDK EEASNTTLTG INKIFYGSNE ITEETLEEGA
     SNKKIVKLSV NGVIADTGES NLFSREQYNH QNFLTQLKKI QEDKAVKGVL LEVNSPGGGI
     YESAEIAKEM AKIKKLDIPI YTAFKNTAAS GGYYISAGSD KIFATEETTT GSIGVIISGL
     NYSGLLEKLG VTDATYKSGA LKDMMSPQHK PSEEENKVIQ EFVMSAYDRF VNVVAKGRNM
     DTNAVKELAD GRIYDGNQAV ENGLVDQIGY SEDALDSLKK EKKLTDATVI EYKNDTTGFA
     SSWLGNKIAE WQGLKATTSD RVISVFEKLG FSDTPKPMYY YGGL