ID   SPR2B_MOUSE             Reviewed;          98 AA.
AC   O70554; Q7TSB2;
DT   15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Small proline-rich protein 2B;
GN   Name=Sprr2b;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/SvJ;
RX   PubMed=9888996; DOI=10.1006/geno.1998.5607;
RA   Song H.J., Poy G., Darwiche N., Lichti U., Kuroki T., Steinert P.M.,
RA   Kartasova T.;
RT   "Mouse Sprr2 genes: a clustered family of genes showing differential
RT   expression in epithelial tissues.";
RL   Genomics 55:28-42(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=12584609; DOI=10.1007/s00335-002-2205-4;
RA   Patel S., Kartasova T., Segre J.A.;
RT   "Mouse Sprr locus: a tandem array of coordinately regulated genes.";
RL   Mamm. Genome 14:140-148(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=CD-1; TISSUE=Uterus;
RA   Tan Y., Sun X., Wang Y.;
RT   "Small proline rich proteins in the reproductive system of female mice.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX   PubMed=15232223;
RA   Hong S.H., Nah H.Y., Lee J.Y., Lee Y.J., Lee J.W., Gye M.C., Kim C.H.,
RA   Kang B.M., Kim M.K.;
RT   "Estrogen regulates the expression of the small proline-rich 2 gene family
RT   in the mouse uterus.";
RL   Mol. Cells 17:477-484(2004).
CC   -!- FUNCTION: Cross-linked envelope protein of keratinocytes. It is a
CC       keratinocyte protein that first appears in the cell cytosol, but
CC       ultimately becomes cross-linked to membrane proteins by
CC       transglutaminase. All that results in the formation of an insoluble
CC       envelope beneath the plasma membrane (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in uterus. {ECO:0000269|PubMed:15232223}.
CC   -!- DEVELOPMENTAL STAGE: Expression in uterus varies during the estrous
CC       cycle, with highest levels during proestrus and estrus stages and
CC       declining sharply from metestrus to diestrus. During early pregnancy,
CC       uterine expression is markedly increased at 1 dpc, declines rapidly at
CC       2 dpc and is almost undetectable from 3 dpc to 6 dpc.
CC       {ECO:0000269|PubMed:15232223}.
CC   -!- INDUCTION: Up-regulated by estrogen in the uterus of ovariectomized
CC       animals, with strongly increased expression detected in luminal
CC       epithelial cells at 6 and 12 hours after hormone injection.
CC       {ECO:0000269|PubMed:15232223}.
CC   -!- SIMILARITY: Belongs to the cornifin (SPRR) family. {ECO:0000305}.
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DR   EMBL; AJ005560; CAA06589.1; -; Genomic_DNA.
DR   EMBL; AY158987; AAN86824.1; -; mRNA.
DR   EMBL; AY298855; AAP57203.1; -; mRNA.
DR   CCDS; CCDS38509.1; -.
DR   AlphaFoldDB; O70554; -.
DR   STRING; 10090.ENSMUSP00000058131; -.
DR   PhosphoSitePlus; O70554; -.
DR   MaxQB; O70554; -.
DR   PaxDb; O70554; -.
DR   PRIDE; O70554; -.
DR   ProteomicsDB; 261628; -.
DR   MGI; MGI:1330352; Sprr2b.
DR   PRO; PR:O70554; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; O70554; protein.
DR   GO; GO:0001533; C:cornified envelope; NAS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008544; P:epidermis development; NAS:UniProtKB.
DR   GO; GO:0031424; P:keratinization; IEA:UniProtKB-KW.
DR   GO; GO:0030216; P:keratinocyte differentiation; NAS:UniProtKB.
DR   GO; GO:0032355; P:response to estradiol; IDA:MGI.
DR   InterPro; IPR029142; SPRR2.
DR   Pfam; PF14820; SPRR2; 2.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Keratinization; Reference proteome; Repeat.
FT   CHAIN           1..98
FT                   /note="Small proline-rich protein 2B"
FT                   /id="PRO_0000150015"
FT   REPEAT          21..29
FT                   /note="1"
FT   REPEAT          30..38
FT                   /note="2"
FT   REPEAT          39..47
FT                   /note="3"
FT   REPEAT          48..56
FT                   /note="4"
FT   REPEAT          57..65
FT                   /note="5"
FT   REGION          21..65
FT                   /note="5 X 9 AA approximate tandem repeats"
FT   CONFLICT        4
FT                   /note="Y -> H (in Ref. 3; AAP57203)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        43..51
FT                   /note="Missing (in Ref. 3; AAP57203)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   98 AA;  10735 MW;  81FB0894FA6CDBCC CRC64;
     MSYYQQQCKQ PCQPPPVCPP PKCPEPCPPP KCPEPCPPPV CCEPCPPPKC PEPCPPPVCC
     EPCPPPVCCE PCPPQPWQPK CPPVQFPPCQ QKCPPKNK