ID   SPRE2_DANRE             Reviewed;         413 AA.
AC   Q6NYK3;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Sprouty-related, EVH1 domain-containing protein 2;
DE            Short=Spred-2;
GN   Name=spred2; ORFNames=zgc:77284;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Negatively regulates Ras signaling pathways and downstream
CC       activation of MAP kinases. {ECO:0000250|UniProtKB:Q7Z698}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q924S7};
CC       Peripheral membrane protein {ECO:0000250|UniProtKB:Q924S7}; Cytoplasmic
CC       side {ECO:0000250|UniProtKB:Q924S7}. Cytoplasmic vesicle, secretory
CC       vesicle membrane {ECO:0000250|UniProtKB:Q7Z698}; Peripheral membrane
CC       protein {ECO:0000250|UniProtKB:Q7Z698}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:Q7Z698}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q7Z698}.
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DR   EMBL; BC066561; AAH66561.1; -; mRNA.
DR   RefSeq; NP_998399.1; NM_213234.1.
DR   AlphaFoldDB; Q6NYK3; -.
DR   SMR; Q6NYK3; -.
DR   STRING; 7955.ENSDARP00000045291; -.
DR   PaxDb; Q6NYK3; -.
DR   GeneID; 793290; -.
DR   KEGG; dre:793290; -.
DR   CTD; 793290; -.
DR   ZFIN; ZDB-GENE-040426-2342; spred2b.
DR   eggNOG; KOG4590; Eukaryota.
DR   InParanoid; Q6NYK3; -.
DR   OrthoDB; 759156at2759; -.
DR   PhylomeDB; Q6NYK3; -.
DR   Reactome; R-DRE-5658442; Regulation of RAS by GAPs.
DR   Reactome; R-DRE-5658623; FGFRL1 modulation of FGFR1 signaling.
DR   PRO; PR:Q6NYK3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR   GO; GO:0043409; P:negative regulation of MAPK cascade; IBA:GO_Central.
DR   CDD; cd10574; EVH1_SPRED-like; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR023337; KBD.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR041937; SPRE_EVH1.
DR   InterPro; IPR007875; Sprouty.
DR   InterPro; IPR000697; WH1/EVH1_dom.
DR   Pfam; PF05210; Sprouty; 1.
DR   Pfam; PF00568; WH1; 1.
DR   SMART; SM00461; WH1; 1.
DR   PROSITE; PS51488; KBD; 1.
DR   PROSITE; PS51227; SPR; 1.
DR   PROSITE; PS50229; WH1; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasm; Cytoplasmic vesicle; Membrane;
KW   Reference proteome.
FT   CHAIN           1..413
FT                   /note="Sprouty-related, EVH1 domain-containing protein 2"
FT                   /id="PRO_0000076913"
FT   DOMAIN          5..122
FT                   /note="WH1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00410"
FT   DOMAIN          195..248
FT                   /note="KBD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00821"
FT   DOMAIN          303..411
FT                   /note="SPR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00572"
FT   REGION          122..163
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        122..137
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        145..163
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   413 AA;  46830 MW;  489E71676A1D73E4 CRC64;
     MIEETHPNDD SYIVRVKAVV MTRDDSSGGW LAQEGGGLSR VGVCKVMPAE LTGRSDFLIH
     GERLKDKQVI LECFVRKDLI YTKATPTFHH WKVDNKKCGL TFQSPADARA FDRGVRKALE
     DLTEGSTTSS STLQNEAELG DDDVFTTATD SSSNSSQKKD HSTQLVATST FYEPHPHRCI
     LQYRPPERYT LDQKFSRNLF PFEDEEIVRI NPRERWMITG YEDYRYAAVP DKFIQPEDSD
     SYVQISKNDP VKHDYTYPYV PSPDFTQCDL KRPCGGGSTV VSTQPRAFIL KGKRRKEDGE
     RSRCVYCRDM FNHEENRRGQ CHDAPDPIRT CIHRVSFMWC ADSMLYHCMS DPEGDYSDPC
     SCDTSEERFC LRWTALLGLA MLAPCLCCYP PLHGCHRCGV ACGCCGGKHK AVG