ID   SPRE2_XENTR             Reviewed;         409 AA.
AC   Q5Y171;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Sprouty-related, EVH1 domain-containing protein 2;
DE            Short=Spred-2;
GN   Name=spred2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Sivak J.M., Petersen L.F., Amaya E.;
RT   "Sprouty and Spred proteins diverge functionally to coordinate FGF signal
RT   interpretation during mesoderm formation.";
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Negatively regulates Ras signaling pathways and downstream
CC       activation of MAP kinases. {ECO:0000250|UniProtKB:Q7Z698}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q924S7};
CC       Peripheral membrane protein {ECO:0000250|UniProtKB:Q924S7}; Cytoplasmic
CC       side {ECO:0000250|UniProtKB:Q924S7}. Cytoplasmic vesicle, secretory
CC       vesicle membrane {ECO:0000250|UniProtKB:Q7Z698}; Peripheral membrane
CC       protein {ECO:0000250|UniProtKB:Q7Z698}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:Q7Z698}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q7Z698}.
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DR   EMBL; AY714337; AAU43766.1; -; mRNA.
DR   RefSeq; NP_001011032.1; NM_001011032.1.
DR   AlphaFoldDB; Q5Y171; -.
DR   SMR; Q5Y171; -.
DR   PaxDb; Q5Y171; -.
DR   GeneID; 496441; -.
DR   KEGG; xtr:496441; -.
DR   CTD; 200734; -.
DR   Xenbase; XB-GENE-485591; spred2.
DR   eggNOG; KOG4590; Eukaryota.
DR   InParanoid; Q5Y171; -.
DR   OrthoDB; 759156at2759; -.
DR   Reactome; R-XTR-5658442; Regulation of RAS by GAPs.
DR   Reactome; R-XTR-5658623; FGFRL1 modulation of FGFR1 signaling.
DR   Proteomes; UP000008143; Chromosome 5.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR   GO; GO:0043409; P:negative regulation of MAPK cascade; IBA:GO_Central.
DR   CDD; cd10574; EVH1_SPRED-like; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR023337; KBD.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR041937; SPRE_EVH1.
DR   InterPro; IPR007875; Sprouty.
DR   InterPro; IPR000697; WH1/EVH1_dom.
DR   Pfam; PF05210; Sprouty; 1.
DR   Pfam; PF00568; WH1; 1.
DR   SMART; SM00461; WH1; 1.
DR   PROSITE; PS51488; KBD; 1.
DR   PROSITE; PS51227; SPR; 1.
DR   PROSITE; PS50229; WH1; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasm; Cytoplasmic vesicle; Membrane;
KW   Reference proteome.
FT   CHAIN           1..409
FT                   /note="Sprouty-related, EVH1 domain-containing protein 2"
FT                   /id="PRO_0000076912"
FT   DOMAIN          5..121
FT                   /note="WH1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00410"
FT   DOMAIN          199..253
FT                   /note="KBD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00821"
FT   DOMAIN          299..407
FT                   /note="SPR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00572"
FT   REGION          121..170
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        144..161
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   409 AA;  46148 MW;  F7578D0E3DA229D8 CRC64;
     MSEEAPPEDD SYIVRVKAVV MSRDDSSGGW LPHEGGGLSR VGVSKVYPEG SGRSNFLIHG
     ERLNDKLVLL ECYLKKDLVY WKATPTFHHW LVDNRRFGLT FQSPADARAF DRGVRKAIED
     LTEGSTTSSS TIHNEAELGD DDVFATSTDS SSNSSQKREP PVRTIASPLP GGHCRYSTLS
     NIDLPGFEHY PTEQSCARPH RHVSFPDDDD EIVRINPRER NWLTGYEDYR QAPIHRKYPD
     TESIDSYVRF AKSESTKHDY NYPYVDHTNF GISKDLKTSV INTQPSRCSS RRKDGERSRC
     IYCRDMFNHD ENRRGQCQDA PDSVRNCIRR VSCMGFADCV LYHCMSDSEG DYTDACSCDT
     SDEKFCLRWM LLIPLSIIAP GMCCYLPLRA CYNCGVMCGC CGGKHKAAG