SPRNG_MOUSE
ID SPRNG_MOUSE Reviewed; 205 AA.
AC Q8BTG6;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=SREBP regulating gene protein {ECO:0000303|PubMed:32111832};
DE AltName: Full=SREBF pathway regulator in Golgi 1;
GN Name=Spring1; Synonyms=Spring {ECO:0000303|PubMed:32111832};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=32111832; DOI=10.1038/s41467-020-14811-1;
RA Loregger A., Raaben M., Nieuwenhuis J., Tan J.M.E., Jae L.T.,
RA van den Hengel L.G., Hendrix S., van den Berg M., Scheij S., Song J.Y.,
RA Huijbers I.J., Kroese L.J., Ottenhoff R., van Weeghel M., van de Sluis B.,
RA Brummelkamp T., Zelcer N.;
RT "Haploid genetic screens identify SPRING/C12ORF49 as a determinant of SREBP
RT signaling and cholesterol metabolism.";
RL Nat. Commun. 11:1128-1128(2020).
CC -!- FUNCTION: Positively regulates hepatic SREBP signaling pathway by
CC modulating the proper localization of SCAP (SREBP cleavage-activating
CC protein) to the endoplasmic reticulum, thereby controlling the level of
CC functional SCAP (PubMed:32111832). Plays a crucial role during
CC embyrogenesis (PubMed:32111832). {ECO:0000269|PubMed:32111832}.
CC -!- SUBUNIT: Interacts with SCAP. {ECO:0000250|UniProtKB:Q9H741}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q9H741}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed with a slightly higher
CC expression in the liver and kidney. {ECO:0000269|PubMed:32111832}.
CC -!- DISRUPTION PHENOTYPE: Knockout leads to early embryonic lethality.
CC {ECO:0000269|PubMed:32111832}.
CC -!- SIMILARITY: Belongs to the SPRING family. {ECO:0000305}.
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DR EMBL; AK090380; BAC41192.1; -; mRNA.
DR EMBL; BC104372; AAI04373.1; -; mRNA.
DR EMBL; BC104373; AAI04374.1; -; mRNA.
DR CCDS; CCDS39237.1; -.
DR RefSeq; NP_001074705.1; NM_001081236.1.
DR AlphaFoldDB; Q8BTG6; -.
DR STRING; 10090.ENSMUSP00000043410; -.
DR GlyGen; Q8BTG6; 1 site.
DR PhosphoSitePlus; Q8BTG6; -.
DR PaxDb; Q8BTG6; -.
DR PeptideAtlas; Q8BTG6; -.
DR PRIDE; Q8BTG6; -.
DR Antibodypedia; 31314; 103 antibodies from 18 providers.
DR Ensembl; ENSMUST00000049138; ENSMUSP00000043410; ENSMUSG00000032840.
DR GeneID; 76792; -.
DR KEGG; mmu:76792; -.
DR UCSC; uc008zgj.1; mouse.
DR CTD; 79794; -.
DR MGI; MGI:1924042; Spring1.
DR VEuPathDB; HostDB:ENSMUSG00000032840; -.
DR eggNOG; KOG3136; Eukaryota.
DR GeneTree; ENSGT00390000008031; -.
DR HOGENOM; CLU_079455_0_0_1; -.
DR InParanoid; Q8BTG6; -.
DR OMA; PEARCRN; -.
DR OrthoDB; 1602813at2759; -.
DR PhylomeDB; Q8BTG6; -.
DR TreeFam; TF323884; -.
DR BioGRID-ORCS; 76792; 21 hits in 72 CRISPR screens.
DR PRO; PR:Q8BTG6; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8BTG6; protein.
DR Bgee; ENSMUSG00000032840; Expressed in lumbar subsegment of spinal cord and 194 other tissues.
DR Genevisible; Q8BTG6; MM.
DR GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:2000640; P:positive regulation of SREBP signaling pathway; IMP:UniProtKB.
DR InterPro; IPR019352; SPRING1.
DR PANTHER; PTHR13481; PTHR13481; 1.
DR Pfam; PF10218; SPRING1; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..205
FT /note="SREBP regulating gene protein"
FT /id="PRO_0000294330"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9H741"
FT TRANSMEM 17..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..205
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9H741"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 205 AA; 23477 MW; 55FF235D30E0A17E CRC64;
MLNLAALLWR RLLRKRWVLA LVFGLSLVYF LSSTFKQEER AVRDRNLLQV QDREQPIPWK
VQFNLGNSSR PSNQCRNSVQ GKHLLTDELG YVCERKDLLA NGCCDVSVPS TKQYCCDGCL
ANGCCEAYEY CVSCCLQPSK QLLLERFLNR AAVAFQNLFM AVEDHFELCL AKCRTSSQSV
QHENTYRDPI AKYCYGESPP ELFPA
