SPRR3_HUMAN
ID SPRR3_HUMAN Reviewed; 169 AA.
AC Q9UBC9; A5YKK8; B2R4G8; D3DV32; O75597; Q4ZGI7; Q5T525; Q8NET7; Q9UDG3;
DT 11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT 11-FEB-2002, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Small proline-rich protein 3;
DE AltName: Full=22 kDa pancornulin;
DE AltName: Full=Cornifin beta;
DE AltName: Full=Esophagin;
GN Name=SPRR3; Synonyms=SPRC;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Peripheral blood;
RX PubMed=8325635; DOI=10.1006/geno.1993.1240;
RA Gibbs S., Fijneman R., Wiegant J., Geurts van Kessel A., van de Putte P.,
RA Backendorf C.;
RT "Molecular characterization and evolution of the SPRR family of
RT keratinocyte differentiation markers encoding small proline-rich
RT proteins.";
RL Genomics 16:630-637(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8018055; DOI=10.1016/0003-9969(94)90051-5;
RA Robinson P.A., Marley J.J., High A.S., Hume W.J.;
RT "Differential expression of protease inhibitor and small proline-rich
RT protein genes between normal human oral tissue and odontogenic
RT keratocysts.";
RL Arch. Oral Biol. 39:251-259(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8809402;
RA Abraham J.M., Wang S., Suzuki H., Jiang H.Y., Rosenblum-Vos L.S., Yin J.,
RA Meltzer S.J.;
RT "Esophagin cDNA cloning and characterization: a tissue-specific member of
RT the small proline-rich protein family that is not expressed in esophageal
RT tumors.";
RL Cell Growth Differ. 7:855-860(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11230159; DOI=10.1101/gr.114801;
RA Marenholz I., Zirra M., Fischer D.F., Backendorf C., Ziegler A.,
RA Mischke D.;
RT "Identification of human epidermal differentiation complex (EDC)-encoded
RT genes by subtractive hybridization of entire YACs to a gridded keratinocyte
RT cDNA library.";
RL Genome Res. 11:341-355(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA], MASS SPECTROMETRY, CLEAVAGE OF INITIATOR
RP METHIONINE, ACETYLATION AT SER-2, AND VARIANTS DEL 98-CYS--PRO-104 AND
RP VAL-149.
RC TISSUE=Oral mucosa, Parotid gland, Saliva, and Submandibular gland;
RX PubMed=20599699; DOI=10.1016/j.bbrc.2010.06.103;
RA Manconi B., Cabras T., Pisano E., Nemolato S., Inzitari R., Iavarone F.,
RA Fanali C., Sanna M.T., Tirone C., Vento G., Romagnoli C., Faa G.,
RA Castagnola M., Messana I.;
RT "Characterization of two isoforms of human SPRR3 from saliva of preterm
RT human newborn and autoptic fetal oral mucosa, parotid and submandibular
RT gland samples.";
RL Biochem. Biophys. Res. Commun. 398:477-481(2010).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT VAL-149.
RG NIEHS SNPs program;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Retina;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [10]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [11]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT VAL-149.
RC TISSUE=Skeletal muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [12]
RP PROTEIN SEQUENCE OF 8-40 AND 140-165.
RC TISSUE=Keratinocyte;
RX PubMed=7829876; DOI=10.1111/1523-1747.ep12612759;
RA Greco M.A., Lorand L., Lane W.S., Baden H.P., Parameswaran K.N.P.,
RA Kvedar J.C.;
RT "The pancornulins: a group of small proline rich-related cornified envelope
RT precursors with bifunctional capabilities in isopeptide bond formation.";
RL J. Invest. Dermatol. 104:204-210(1995).
CC -!- FUNCTION: Cross-linked envelope protein of keratinocytes.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- INDUCTION: During squamous differentiation of epidermal keratinocytes.
CC -!- MASS SPECTROMETRY: Mass=17239; Method=Electrospray; Note=Variants Del
CC 98-Cys--Pro-105 and Val-149.; Evidence={ECO:0000269|PubMed:20599699};
CC -!- MASS SPECTROMETRY: Mass=18065; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:20599699};
CC -!- SIMILARITY: Belongs to the cornifin (SPRR) family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=NIEHS-SNPs;
CC URL="http://egp.gs.washington.edu/data/sprr3/";
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DR EMBL; AF077374; AAD11938.1; -; Genomic_DNA.
DR EMBL; AY118269; AAM74559.1; -; mRNA.
DR EMBL; AJ243667; CAB65098.1; -; mRNA.
DR EMBL; DQ017955; AAY26394.1; -; Genomic_DNA.
DR EMBL; AK311823; BAG34765.1; -; mRNA.
DR EMBL; EF553525; ABQ66270.1; -; mRNA.
DR EMBL; AL356867; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471121; EAW53353.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW53354.1; -; Genomic_DNA.
DR EMBL; BC017802; AAH17802.1; -; mRNA.
DR CCDS; CCDS1033.1; -.
DR RefSeq; NP_001091058.1; NM_001097589.1.
DR RefSeq; NP_005407.1; NM_005416.2.
DR AlphaFoldDB; Q9UBC9; -.
DR BioGRID; 112585; 54.
DR IntAct; Q9UBC9; 4.
DR MINT; Q9UBC9; -.
DR STRING; 9606.ENSP00000330391; -.
DR ChEMBL; CHEMBL4295970; -.
DR iPTMnet; Q9UBC9; -.
DR PhosphoSitePlus; Q9UBC9; -.
DR BioMuta; SPRR3; -.
DR EPD; Q9UBC9; -.
DR jPOST; Q9UBC9; -.
DR MassIVE; Q9UBC9; -.
DR MaxQB; Q9UBC9; -.
DR PaxDb; Q9UBC9; -.
DR PeptideAtlas; Q9UBC9; -.
DR PRIDE; Q9UBC9; -.
DR ProteomicsDB; 83944; -.
DR Antibodypedia; 34110; 196 antibodies from 28 providers.
DR DNASU; 6707; -.
DR Ensembl; ENST00000295367.5; ENSP00000295367.4; ENSG00000163209.15.
DR Ensembl; ENST00000331860.7; ENSP00000330391.3; ENSG00000163209.15.
DR GeneID; 6707; -.
DR KEGG; hsa:6707; -.
DR MANE-Select; ENST00000295367.5; ENSP00000295367.4; NM_001097589.2; NP_001091058.1.
DR UCSC; uc001fax.5; human.
DR CTD; 6707; -.
DR DisGeNET; 6707; -.
DR GeneCards; SPRR3; -.
DR HGNC; HGNC:11268; SPRR3.
DR HPA; ENSG00000163209; Group enriched (esophagus, vagina).
DR MIM; 182271; gene.
DR neXtProt; NX_Q9UBC9; -.
DR OpenTargets; ENSG00000163209; -.
DR PharmGKB; PA36097; -.
DR VEuPathDB; HostDB:ENSG00000163209; -.
DR eggNOG; ENOG502SDIP; Eukaryota.
DR GeneTree; ENSGT00940000164092; -.
DR HOGENOM; CLU_101829_1_0_1; -.
DR InParanoid; Q9UBC9; -.
DR OMA; DKGCTKV; -.
DR OrthoDB; 1603381at2759; -.
DR PhylomeDB; Q9UBC9; -.
DR TreeFam; TF338205; -.
DR PathwayCommons; Q9UBC9; -.
DR Reactome; R-HSA-6809371; Formation of the cornified envelope.
DR SignaLink; Q9UBC9; -.
DR BioGRID-ORCS; 6707; 9 hits in 1057 CRISPR screens.
DR ChiTaRS; SPRR3; human.
DR GeneWiki; SPRR3; -.
DR GenomeRNAi; 6707; -.
DR Pharos; Q9UBC9; Tbio.
DR PRO; PR:Q9UBC9; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9UBC9; protein.
DR Bgee; ENSG00000163209; Expressed in lower esophagus mucosa and 110 other tissues.
DR ExpressionAtlas; Q9UBC9; baseline and differential.
DR Genevisible; Q9UBC9; HS.
DR GO; GO:0001533; C:cornified envelope; TAS:Reactome.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:BHF-UCL.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
DR GO; GO:0005198; F:structural molecule activity; TAS:UniProtKB.
DR GO; GO:0008544; P:epidermis development; NAS:UniProtKB.
DR GO; GO:0031424; P:keratinization; IEA:UniProtKB-KW.
DR GO; GO:0030216; P:keratinocyte differentiation; NAS:UniProtKB.
DR GO; GO:0018149; P:peptide cross-linking; IEA:InterPro.
DR GO; GO:0042060; P:wound healing; TAS:UniProtKB.
DR InterPro; IPR003302; SPRR1/SPRR3.
DR PANTHER; PTHR23263:SF28; PTHR23263:SF28; 2.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Direct protein sequencing; Keratinization;
KW Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:20599699"
FT CHAIN 2..169
FT /note="Small proline-rich protein 3"
FT /id="PRO_0000150003"
FT REPEAT 43..50
FT /note="1"
FT REPEAT 51..58
FT /note="2"
FT REPEAT 59..66
FT /note="3"
FT REPEAT 67..74
FT /note="4"
FT REPEAT 75..82
FT /note="5"
FT REPEAT 83..90
FT /note="6"
FT REPEAT 91..98
FT /note="7"
FT REPEAT 99..106
FT /note="8"
FT REPEAT 107..114
FT /note="9"
FT REPEAT 115..122
FT /note="10"
FT REPEAT 123..130
FT /note="11"
FT REPEAT 131..138
FT /note="12"
FT REPEAT 139..146
FT /note="13"
FT REPEAT 147..154
FT /note="14"
FT REGION 1..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 43..154
FT /note="14 X 8 AA approximate tandem repeats"
FT REGION 150..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000269|PubMed:20599699"
FT VARIANT 98..105
FT /note="Missing"
FT /id="VAR_065255"
FT VARIANT 149
FT /note="L -> V (in dbSNP:rs1055935)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:20599699, ECO:0000269|Ref.6"
FT /id="VAR_023377"
FT VARIANT 156
FT /note="T -> M (in dbSNP:rs2075740)"
FT /id="VAR_053048"
FT CONFLICT 19
FT /note="Q -> H (in Ref. 3; AAM74559)"
FT /evidence="ECO:0000305"
FT CONFLICT 61..68
FT /note="Missing (in Ref. 2; no nucleotide entry, 4; CAB65098
FT and 9; AAH17802)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 169 AA; 18154 MW; B8E522AF798F1ECB CRC64;
MSSYQQKQTF TPPPQLQQQQ VKQPSQPPPQ EIFVPTTKEP CHSKVPQPGN TKIPEPGCTK
VPEPGCTKVP EPGCTKVPEP GCTKVPEPGC TKVPEPGCTK VPEPGYTKVP EPGSIKVPDQ
GFIKFPEPGA IKVPEQGYTK VPVPGYTKLP EPCPSTVTPG PAQQKTKQK
