SPS18_YEAST
ID SPS18_YEAST Reviewed; 300 AA.
AC P32572; D6W0Y5;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Sporulation protein SPS18;
DE AltName: Full=Sporulation-specific protein SPX18;
GN Name=SPS18; Synonyms=SPX18; OrderedLocusNames=YNL204C; ORFNames=N1354;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=7725799; DOI=10.1002/yea.320101213;
RA Jonniaux J.-L., Coster F., Purnelle B., Goffeau A.;
RT "A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries
RT WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8
RT new open reading frames of unknown function.";
RL Yeast 10:1639-1645(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-266.
RC STRAIN=ATCC 28684 / S14;
RX PubMed=7969036; DOI=10.1007/bf00282757;
RA Coe J.G., Murray L.E., Dawes I.W.;
RT "Identification of a sporulation-specific promoter regulating divergent
RT transcription of two novel sporulation genes in Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 244:661-672(1994).
CC -!- DEVELOPMENTAL STAGE: Sequentially activated during the process of
CC meiosis and spore formation.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-10 is the initiator.
CC {ECO:0000305}.
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DR EMBL; X78898; CAA55504.1; -; Genomic_DNA.
DR EMBL; Z71482; CAA96108.1; -; Genomic_DNA.
DR EMBL; AY693126; AAT93145.1; -; Genomic_DNA.
DR EMBL; M90351; AAA62404.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10351.1; -; Genomic_DNA.
DR PIR; S50727; S50727.
DR RefSeq; NP_014195.1; NM_001183042.1.
DR AlphaFoldDB; P32572; -.
DR SMR; P32572; -.
DR BioGRID; 35631; 39.
DR DIP; DIP-2859N; -.
DR IntAct; P32572; 5.
DR MINT; P32572; -.
DR STRING; 4932.YNL204C; -.
DR PaxDb; P32572; -.
DR PRIDE; P32572; -.
DR EnsemblFungi; YNL204C_mRNA; YNL204C; YNL204C.
DR GeneID; 855517; -.
DR KEGG; sce:YNL204C; -.
DR SGD; S000005148; SPS18.
DR VEuPathDB; FungiDB:YNL204C; -.
DR eggNOG; KOG0704; Eukaryota.
DR HOGENOM; CLU_070893_0_0_1; -.
DR InParanoid; P32572; -.
DR OMA; CVNCANL; -.
DR BioCyc; YEAST:G3O-33211-MON; -.
DR Reactome; R-SCE-6807878; COPI-mediated anterograde transport.
DR Reactome; R-SCE-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:P32572; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P32572; protein.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030437; P:ascospore formation; IMP:SGD.
DR GO; GO:0032012; P:regulation of ARF protein signal transduction; IBA:GO_Central.
DR GO; GO:0030100; P:regulation of endocytosis; IBA:GO_Central.
DR Gene3D; 1.10.220.150; -; 1.
DR InterPro; IPR037278; ARFGAP/RecO.
DR InterPro; IPR001164; ArfGAP_dom.
DR InterPro; IPR038508; ArfGAP_dom_sf.
DR Pfam; PF01412; ArfGap; 1.
DR PRINTS; PR00405; REVINTRACTNG.
DR SMART; SM00105; ArfGap; 1.
DR SUPFAM; SSF57863; SSF57863; 1.
DR PROSITE; PS50115; ARFGAP; 1.
PE 2: Evidence at transcript level;
KW GTPase activation; Metal-binding; Reference proteome; Sporulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..300
FT /note="Sporulation protein SPS18"
FT /id="PRO_0000074227"
FT DOMAIN 11..130
FT /note="Arf-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT ZN_FING 28..51
FT /note="C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT CONFLICT 106
FT /note="R -> P (in Ref. 5; AAA62404)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="R -> Q (in Ref. 5; AAA62404)"
FT /evidence="ECO:0000305"
FT CONFLICT 201
FT /note="D -> N (in Ref. 5; AAA62404)"
FT /evidence="ECO:0000305"
FT CONFLICT 230
FT /note="Q -> V (in Ref. 5; AAA62404)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 300 AA; 34240 MW; D4FEFF8E239F89D7 CRC64;
MRLFENSKDM ENRKRLLRAK KAAGNNNCFE CKSVNPQFVS CSFGIFICVN CANLLRGMGT
NIFCVKSITM DNFEEKDVRR VEKSGNNRFG SFLSKNGILQ NGIPLREKYD NLFAKSYKRR
LANEVRSNDI NRNMYLGFNN FQQYTNGATS QIRDRTLREI SNNSNASEGA EFVLPEKVLG
SDNFQDCERF PACLSSERNL DENNVTSATS TLTIEKFQND PIGTISRSWQ LLSDALYKSY
EDFKGSVVQP TIENIQQRNL PNDIKRSFVH FNEKLHETPH LPSPVFSCFT GGDILPPEFN
