SPSA1_ORYSI
ID SPSA1_ORYSI Reviewed; 1084 AA.
AC A2WYE9; Q43010; Q43802; Q5JLN3; Q94JN0;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Probable sucrose-phosphate synthase 1;
DE EC=2.4.1.14;
DE AltName: Full=UDP-glucose-fructose-phosphate glucosyltransferase;
GN Name=SPS1; ORFNames=OsI_004842;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. IR36; TISSUE=Leaf;
RX PubMed=8666248; DOI=10.1016/0378-1119(95)00854-3;
RA Valdez-Alarcon J.J., Ferrando M., Jimenez-Moraila B., Herrera-Estrella L.;
RT "Characterization of a rice sucrose-phosphate synthase-encoding gene.";
RL Gene 170:217-222(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=11027714; DOI=10.1104/pp.124.2.641;
RA Chavez-Barcenas A.T., Valdez-Alarcon J.J., Martinez-Trujillo M., Chen L.,
RA Xoconostle-Cazares B., Lucas W.J., Herrera-Estrella L.;
RT "Tissue-specific and developmental pattern of expression of the rice sps1
RT gene.";
RL Plant Physiol. 124:641-654(2000).
CC -!- FUNCTION: Plays a role in photosynthetic sucrose synthesis by
CC catalyzing the rate-limiting step of sucrose biosynthesis from UDP-
CC glucose and fructose- 6-phosphate. Involved in the regulation of carbon
CC partitioning in the leaves of plants. May regulate the synthesis of
CC sucrose and therefore play a major role as a limiting factor in the
CC export of photoassimilates out of the leaf. Plays a role for sucrose
CC availability that is essential for plant growth and fiber elongation
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = H(+) +
CC sucrose 6(F)-phosphate + UDP; Xref=Rhea:RHEA:22172,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57634, ChEBI:CHEBI:57723,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.14;
CC -!- ACTIVITY REGULATION: Activity is regulated by phosphorylation and
CC moderated by concentration of metabolites and light. {ECO:0000250}.
CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC fructose 6-phosphate and UDP-alpha-D-glucose: step 1/2.
CC -!- SUBUNIT: Homodimer or homotetramer. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves mesophyll cells, scutellum of
CC germinating seedlings and pollen of immature inflorescences.
CC {ECO:0000269|PubMed:11027714, ECO:0000269|PubMed:8666248}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC49379.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAC49379.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; U33175; AAC49379.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM000126; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; JC4783; JC4783.
DR AlphaFoldDB; A2WYE9; -.
DR SMR; A2WYE9; -.
DR STRING; 39946.A2WYE9; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR iPTMnet; A2WYE9; -.
DR PRIDE; A2WYE9; -.
DR BioCyc; MetaCyc:MON-1762; -.
DR BRENDA; 2.4.1.14; 4460.
DR UniPathway; UPA00371; UER00545.
DR Proteomes; UP000007015; Chromosome 1.
DR GO; GO:0016157; F:sucrose synthase activity; IEA:InterPro.
DR GO; GO:0046524; F:sucrose-phosphate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd16419; HAD_SPS; 1.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR006380; SPP_N.
DR InterPro; IPR044161; SPS.
DR InterPro; IPR035659; SPS_C.
DR InterPro; IPR012819; SPS_pln.
DR InterPro; IPR000368; Sucrose_synth.
DR PANTHER; PTHR46039; PTHR46039; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR Pfam; PF05116; S6PP; 1.
DR Pfam; PF00862; Sucrose_synth; 1.
DR TIGRFAMs; TIGR02468; sucrsPsyn_pln; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..1084
FT /note="Probable sucrose-phosphate synthase 1"
FT /id="PRO_0000303664"
FT REGION 25..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1084 AA; 119348 MW; A42BA599C4DF4556 CRC64;
MAGNEWINGY LEAILDSGGA AGGGGGGGGG GGGGGGGGGG GGGGGVDPRS PAAGAASPRG
PHMNFNPTHY FVEEVVKGVD ESDLHRTWIK VVATRNARER STRLENMCWR IWHLARKKKQ
LELEGILRIS ARRKEQEQVR RETSEDLAED LFEGEKADTV GELAQQDTPM KKKFQRNFSE
LTVSWSDENK EKKLYIVLIS LHGLVRGDNM ELGRDSDTGG QVKYVVELAR ALAMMPGVYR
VDLFTRQVSS PEVDWSYGEP TEMLTSGSTD GEGSGESAGA YIVRIPCGPR DKYLRKEALW
PYLQEFVDGA LAHILNMSKA LGEQVSNGKL VLPYVIHGHY ADAGDVAALL SGALNVPMVL
TGHSLGRNKL EQIMKQGRMS KEEIDSTYKI MRRIEGEELA LDAAELVITS TRQEIDEQWG
LYDGFDVKLE KVLRARARRG VSCHGRFMPR MVVIPPGMDF SSVVVPEDTS DGDDGKDFEI
ASPRSLPPIW AEVMRFLTNP HKPMILALSR PDPKKNITTL VKAFGECRPL RELANLILIM
GNRDDIDEMS AGNASVLTTV LKLIDKYDLY GSVAFPKHHK QSDVPEIYRL TGKMKGVFIN
PALVEPFGLT LIEAAAHGLP IVATKNGGPV DIKNALNNGL LVDPHDQHAI ADALLKLVAD
KNLWQECRKN GLRNIQLYSW PEHCRTYLTR IAGCRIRNPR WLMDTPADAA AEEEEALEDS
LMDVQDLSLR LSIDGERGSS MNDAPSSDPQ DSVQRIMNKI KRSSPADTDG AKIPAEAAAT
ATSGAMNKYP LLRRRRRLFV IAVDCYGDDG SASKRMLQVI QEVFRAVRSD SQMSRISGFA
LSTAMPLPET LKLLQLGKIP PTDFDALICG SGSEVYYPST AQCVDAGGRL RPDQDYLLHI
NHRWSHDGAK QTIAKLAHDG SGTNVEPDVE SCNPHCVSFF IKDPNKVRTI DEMRERVRMR
GLRCHLMYCR NATRLQVVPL LASRSQALRY LFVRWGLSVG NMYLIVGEHG DTDHEEMLSG
LHKTVIIRGV TEKGSEQLVR SSGSYQREDV VPSESPLIAF TKGDLKADEI MRALKEVTKA
ASGM
