ID   SPSA2_ORYSJ             Reviewed;         963 AA.
AC   B7F7B9; A0A0P0VFM9; Q6H881;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 2.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Probable sucrose-phosphate synthase 2;
DE            EC=2.4.1.14;
DE   AltName: Full=Sucrose phosphate synthase 2F;
DE            Short=OsSPS2F;
DE   AltName: Full=UDP-glucose-fructose-phosphate glucosyltransferase;
GN   Name=SPS2; OrderedLocusNames=Os02g0184400, LOC_Os02g09170;
GN   ORFNames=OJ1572_F02.13, OsJ_05652;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX   PubMed=21683881; DOI=10.1016/j.plantsci.2011.04.019;
RA   Okamura M., Aoki N., Hirose T., Yonekura M., Ohto C., Ohsugi R.;
RT   "Tissue specificity and diurnal change in gene expression of the sucrose
RT   phosphate synthase gene family in rice.";
RL   Plant Sci. 181:159-166(2011).
CC   -!- FUNCTION: Plays a role in photosynthetic sucrose synthesis by
CC       catalyzing the rate-limiting step of sucrose biosynthesis from UDP-
CC       glucose and fructose- 6-phosphate. Involved in the regulation of carbon
CC       partitioning in the leaves of plants. May regulate the synthesis of
CC       sucrose and therefore play a major role as a limiting factor in the
CC       export of photoassimilates out of the leaf. Plays a role for sucrose
CC       availability that is essential for plant growth and fiber elongation
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = H(+) +
CC         sucrose 6(F)-phosphate + UDP; Xref=Rhea:RHEA:22172,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57634, ChEBI:CHEBI:57723,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.14;
CC   -!- ACTIVITY REGULATION: Activity is regulated by phosphorylation and
CC       moderated by concentration of metabolites and light. {ECO:0000250}.
CC   -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC       fructose 6-phosphate and UDP-alpha-D-glucose: step 1/2.
CC   -!- SUBUNIT: Homodimer or homotetramer. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in germinating seeds.
CC       {ECO:0000269|PubMed:21683881}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in source leaves and sink leaves.
CC       {ECO:0000269|PubMed:21683881}.
CC   -!- INDUCTION: Circadian-regulated, with the highest expression 1 hour
CC       after the beginning of dark period (in 14 hours light/10 hours dark
CC       cycle). {ECO:0000269|PubMed:21683881}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF08022.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAH00517.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AP004066; BAD25068.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF08022.2; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014958; BAS77334.1; -; Genomic_DNA.
DR   EMBL; CM000139; EEE56455.1; -; Genomic_DNA.
DR   EMBL; AK121486; BAH00517.1; ALT_INIT; mRNA.
DR   RefSeq; XP_015626343.1; XM_015770857.1.
DR   AlphaFoldDB; B7F7B9; -.
DR   SMR; B7F7B9; -.
DR   STRING; 4530.OS02T0184400-01; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   iPTMnet; B7F7B9; -.
DR   PaxDb; B7F7B9; -.
DR   PRIDE; B7F7B9; -.
DR   EnsemblPlants; Os02t0184400-02; Os02t0184400-02; Os02g0184400.
DR   EnsemblPlants; Os02t0184400-03; Os02t0184400-03; Os02g0184400.
DR   GeneID; 4328527; -.
DR   Gramene; Os02t0184400-02; Os02t0184400-02; Os02g0184400.
DR   Gramene; Os02t0184400-03; Os02t0184400-03; Os02g0184400.
DR   KEGG; osa:4328527; -.
DR   eggNOG; KOG0853; Eukaryota.
DR   HOGENOM; CLU_009583_24_0_1; -.
DR   InParanoid; B7F7B9; -.
DR   OrthoDB; 101620at2759; -.
DR   BRENDA; 2.4.1.14; 8948.
DR   PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR   UniPathway; UPA00371; UER00545.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   ExpressionAtlas; B7F7B9; baseline and differential.
DR   Genevisible; B7F7B9; OS.
DR   GO; GO:0016157; F:sucrose synthase activity; IEA:InterPro.
DR   GO; GO:0046524; F:sucrose-phosphate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd16419; HAD_SPS; 1.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR006380; SPP_N.
DR   InterPro; IPR044161; SPS.
DR   InterPro; IPR035659; SPS_C.
DR   InterPro; IPR012819; SPS_pln.
DR   InterPro; IPR000368; Sucrose_synth.
DR   PANTHER; PTHR46039; PTHR46039; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   Pfam; PF05116; S6PP; 1.
DR   Pfam; PF00862; Sucrose_synth; 1.
DR   TIGRFAMs; TIGR02468; sucrsPsyn_pln; 1.
PE   2: Evidence at transcript level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..963
FT                   /note="Probable sucrose-phosphate synthase 2"
FT                   /id="PRO_0000413641"
FT   REGION          111..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   963 AA;  107986 MW;  610BC79E8459A805 CRC64;
     MAGNDNWINS YLDAILDAGK AAIGGDRPSL LLRERGHFSP ARYFVEEVIT GYDETDLYKT
     WLRANAMRSP QERNTRLENM TWRIWNLARK KKEFEKEEAC RLLKRQPEAE KLRTDTNADM
     SEDLFEGEKG EDAGDPSVAY GDSTTGSSPK TSSIDKLYIV LISLHGLVRG ENMELGRDSD
     TGGQVKYVVE LAKALSSSPG VYRVDLLTRQ ILAPNFDRSY GEPTEMLVST SFKNSKQEKG
     ENSGAYIIRI PFGPKDKYLA KEHLWPFIQE FVDGALGHIV RMSKTIGEEI GCGHPVWPAV
     IHGHYASAGI AAALLSGSLN IPMAFTGHFL GKDKLEGLLK QGRHSREQIN MTYKIMCRIE
     AEELSLDASE IVIASTRQEI EEQWNLYDGF EVILARKLRA RVKRGANCYG RYMPRMVIIP
     PGVEFGHIIH DFEMDGEEEN PCPASEDPPI WSQIMRFFTN PRKPMILAVA RPYPEKNITS
     LVKAFGECRP LRELANLTLI MGNREAISKM NNMSAAVLTS VLTLIDEYDL YGQVAYPKHH
     KHSEVPDIYR LAARTKGAFV NVAYFEQFGV TLIEAAMNGL PIIATKNGAP VEINQVLNNG
     LLVDPHDQNA IADALYKLLS DKQLWSRCRE NGLKNIHQFS WPEHCKNYLS RILTLGPRSP
     AIGGKQEQKA PISGRKHIIV ISVDSVNKED LVRIIRNTIE VTRTEKMSGS TGFVLSTSLT
     ISEIRSLLVS AGMLPTVFDA FICNSGSNIY YPLYSGDTPS SSQVTPAIDQ NHQAHIEYRW
     GGEGLRKYLV KWATSVVERK GRIERQIIFE DPEHSSTYCL AFRVVNPNHL PPLKELRKLM
     RIQSLRCNAL YNHSATRLSV VPIHASRSQA LRYLCIRWGI ELPNVAVLVG ESGDSDYEEL
     LGGLHRTVIL KGEFNIPANR IHTVRRYPLQ DVVALDSSNI IGIEGYSTDD MKSALQQIGV
     LTQ