SPSA4_ORYSJ
ID SPSA4_ORYSJ Reviewed; 1066 AA.
AC Q6ZHZ1; A0A0P0XEF8; Q6UU32;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Probable sucrose-phosphate synthase 4;
DE EC=2.4.1.14;
DE AltName: Full=Sucrose phosphate synthase 4F;
DE Short=OsSPS4F;
DE AltName: Full=UDP-glucose-fructose-phosphate glucosyltransferase;
GN Name=SPS4; Synonyms=SPS8; OrderedLocusNames=Os08g0301500, LOC_Os08g20660;
GN ORFNames=OJ1115_A07.105;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14716315; DOI=10.1038/ng1289;
RA Nagaki K., Cheng Z., Ouyang S., Talbert P.B., Kim M., Jones K.M.,
RA Henikoff S., Buell C.R., Jiang J.;
RT "Sequencing of a rice centromere uncovers active genes.";
RL Nat. Genet. 36:138-145(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=16876912; DOI=10.1016/j.jplph.2006.04.014;
RA Lutfiyya L.L., Xu N., D'Ordine R.L., Morrell J.A., Miller P.W., Duff S.M.;
RT "Phylogenetic and expression analysis of sucrose phosphate synthase
RT isozymes in plants.";
RL J. Plant Physiol. 164:923-933(2007).
RN [7]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=21683881; DOI=10.1016/j.plantsci.2011.04.019;
RA Okamura M., Aoki N., Hirose T., Yonekura M., Ohto C., Ohsugi R.;
RT "Tissue specificity and diurnal change in gene expression of the sucrose
RT phosphate synthase gene family in rice.";
RL Plant Sci. 181:159-166(2011).
CC -!- FUNCTION: Plays a role in photosynthetic sucrose synthesis by
CC catalyzing the rate-limiting step of sucrose biosynthesis from UDP-
CC glucose and fructose- 6-phosphate. Involved in the regulation of carbon
CC partitioning in the leaves of plants. May regulate the synthesis of
CC sucrose and therefore play a major role as a limiting factor in the
CC export of photoassimilates out of the leaf. Plays a role for sucrose
CC availability that is essential for plant growth and fiber elongation
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = H(+) +
CC sucrose 6(F)-phosphate + UDP; Xref=Rhea:RHEA:22172,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57634, ChEBI:CHEBI:57723,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.14;
CC -!- ACTIVITY REGULATION: Activity is regulated by phosphorylation and
CC moderated by concentration of metabolites and light. {ECO:0000250}.
CC -!- PATHWAY: Glycan biosynthesis; sucrose biosynthesis; sucrose from D-
CC fructose 6-phosphate and UDP-alpha-D-glucose: step 1/2.
CC -!- SUBUNIT: Homodimer or homotetramer. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in germinating seeds.
CC {ECO:0000269|PubMed:21683881}.
CC -!- DEVELOPMENTAL STAGE: Expressed in source leaves and sink leaves.
CC {ECO:0000269|PubMed:21683881}.
CC -!- INDUCTION: Circadian-regulated, with the highest expression 1 hour
CC after the beginning of dark period (in 14 hours light/10 hours dark
CC cycle). {ECO:0000269|PubMed:21683881}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ56529.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY360393; AAQ56529.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP004041; BAC92378.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23409.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT04814.1; -; Genomic_DNA.
DR EMBL; AK101676; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015650062.1; XM_015794576.1.
DR RefSeq; XP_015650063.1; XM_015794577.1.
DR AlphaFoldDB; Q6ZHZ1; -.
DR SMR; Q6ZHZ1; -.
DR STRING; 4530.OS08T0301500-01; -.
DR CAZy; GT4; Glycosyltransferase Family 4.
DR iPTMnet; Q6ZHZ1; -.
DR PaxDb; Q6ZHZ1; -.
DR PRIDE; Q6ZHZ1; -.
DR EnsemblPlants; Os08t0301500-01; Os08t0301500-01; Os08g0301500.
DR GeneID; 4345220; -.
DR Gramene; Os08t0301500-01; Os08t0301500-01; Os08g0301500.
DR KEGG; osa:4345220; -.
DR eggNOG; KOG0853; Eukaryota.
DR HOGENOM; CLU_009583_24_0_1; -.
DR InParanoid; Q6ZHZ1; -.
DR OMA; AWSKGIS; -.
DR OrthoDB; 101620at2759; -.
DR BRENDA; 2.4.1.14; 8948.
DR PlantReactome; R-OSA-1119465; Sucrose biosynthesis.
DR UniPathway; UPA00371; UER00545.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q6ZHZ1; OS.
DR GO; GO:0016157; F:sucrose synthase activity; IEA:InterPro.
DR GO; GO:0046524; F:sucrose-phosphate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0005986; P:sucrose biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd16419; HAD_SPS; 1.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR006380; SPP_N.
DR InterPro; IPR044161; SPS.
DR InterPro; IPR035659; SPS_C.
DR InterPro; IPR012819; SPS_pln.
DR InterPro; IPR000368; Sucrose_synth.
DR PANTHER; PTHR46039; PTHR46039; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR Pfam; PF05116; S6PP; 1.
DR Pfam; PF00862; Sucrose_synth; 1.
DR TIGRFAMs; TIGR02468; sucrsPsyn_pln; 1.
PE 2: Evidence at transcript level;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..1066
FT /note="Probable sucrose-phosphate synthase 4"
FT /id="PRO_0000413643"
FT REGION 132..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 688..714
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..165
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 690..714
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 527
FT /note="N -> S (in Ref. 5; AK101676)"
FT /evidence="ECO:0000305"
FT CONFLICT 615
FT /note="A -> T (in Ref. 5; AK101676)"
FT /evidence="ECO:0000305"
FT CONFLICT 900
FT /note="E -> G (in Ref. 5; AK101676)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1066 AA; 118725 MW; AB4AE780FEA070A2 CRC64;
MAGNDWINSY LEAILDAGGA AGEISAAAGG GGDGAAATGE KRDKSSLMLR ERGRFSPARY
FVEEVISGFD ETDLYKTWVR TAAMRSPQER NTRLENMSWR IWNLARKKKQ IEGEEASRLA
KQRLEREKAR RYAAADMSED LSEGEKGENI NESSSTHDES TRGRMPRIGS TDAIEAWASQ
HKDKKLYIVL ISIHGLIRGE NMELGRDSDT GGQVKYVVEL ARALGSTPGV YRVDLLTRQI
SAPDVDWSYG EPTEMLSPRN SENFGHDMGE SSGAYIVRIP FGPRDKYIPK EHLWPHIQEF
VDGALVHIMQ MSKVLGEQVG SGQLVWPVVI HGHYADAGDS AALLSGALNV PMIFTGHSLG
RDKLEQLLKQ GRQTRDEINT IYKIMRRIEA EELCLDASEI IITSTRQEIE QQWGLYDGFD
LTMARKLRAR IKRGVSCYGR YMPRMIAVPP GMEFSHIVPH DVDQDGEEAN EDGSGSTDPP
IWADIMRFFS NPRKPMILAL ARPDPKKNIT TLVKAFGEHR ELRNLANLTL IMGNRDVIDE
MSSTNSAVLT SILKLIDKYD LYGQVAYPKH HKQSEVPDIY RLAARTKGVF INCAFIEPFG
LTLIEAAAYG LPMVATRNGG PVDIHRVLDN GILVDPHNQN EIAEALYKLV SDKQLWAQCR
QNGLKNIHQF SWPEHCKNYL SRVGTLKPRH PRWQKSDDAT EVSEADSPGD SLRDVHDISL
NLKLSLDSEK SSTKENSVRR NLEDAVQKLS RGVSANRKTE SVENMEATTG NKWPSLRRRK
HIVVIAIDSV QDANLVEIIK NIFVASSNER LSGSVGFVLS TSRAISEVHS LLTSGGIEAT
DFDAFICNSG SDLCYPSSNS EDMLSPAELP FMIDLDYHTQ IEYRWGGEGL RKTLICWAAE
KSEGGQVVLV EDEECSSTYC ISFRVKNAEA VPPVKELRKT MRIQALRCHV LYSHDGSKLN
VIPVLASRSQ ALRYLYIRWG VELSNMTVVV GESGDTDYEG LLGGVHKTII LKGSFNAVPN
QVHAARSYSL QDVISFDKPG ITSIEGYGPD NLKSALQQFG ILKDNV
