SPSB2_RAT
ID SPSB2_RAT Reviewed; 264 AA.
AC Q5M877;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=SPRY domain-containing SOCS box protein 2;
DE Short=SSB-2;
GN Name=Spsb2; Synonyms=Ssb2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Substrate recognition component of a SCF-like ECS (Elongin
CC BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which
CC mediates the ubiquitination and subsequent proteasomal degradation of
CC target proteins (By similarity). Negatively regulates nitric oxide (NO)
CC production and limits cellular toxicity in activated macrophages by
CC mediating the ubiquitination and proteasomal degradation of NOS2 (By
CC similarity). Acts as a bridge which links NOS2 with the ECS E3
CC ubiquitin ligase complex components ELOC and CUL5 (By similarity).
CC {ECO:0000250|UniProtKB:Q99619}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of the probable ECS(SPSB2) E3 ubiquitin-protein
CC ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and
CC SPSB2 (By similarity). Interacts with CUL5, RNF7, ELOB and ELOC (By
CC similarity). Interacts with MET (By similarity). Interacts (via
CC B30.2/SPRY domain) with PAWR; this interaction occurs in association
CC with the Elongin BC complex (By similarity). Interacts with NOS2 (By
CC similarity). {ECO:0000250|UniProtKB:Q99619}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q99619}. Note=Exhibits a diffuse cytosolic
CC localization. {ECO:0000250|UniProtKB:Q99619}.
CC -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC BC complex, an adapter module in different E3 ubiquitin ligase
CC complexes (By similarity). Essential for its ability to link NOS2 and
CC the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By
CC similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q99619}.
CC -!- SIMILARITY: Belongs to the SPSB family. {ECO:0000305}.
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DR EMBL; BC088189; AAH88189.1; -; mRNA.
DR RefSeq; NP_001009660.1; NM_001009660.1.
DR RefSeq; XP_003750703.1; XM_003750655.4.
DR AlphaFoldDB; Q5M877; -.
DR SMR; Q5M877; -.
DR STRING; 10116.ENSRNOP00000020493; -.
DR PaxDb; Q5M877; -.
DR Ensembl; ENSRNOT00000020493; ENSRNOP00000020493; ENSRNOG00000015125.
DR GeneID; 297592; -.
DR KEGG; rno:297592; -.
DR UCSC; RGD:1310854; rat.
DR CTD; 84727; -.
DR RGD; 1310854; Spsb2.
DR eggNOG; KOG3953; Eukaryota.
DR GeneTree; ENSGT01030000234629; -.
DR HOGENOM; CLU_046756_0_0_1; -.
DR InParanoid; Q5M877; -.
DR OMA; HAWEIGW; -.
DR OrthoDB; 938259at2759; -.
DR PhylomeDB; Q5M877; -.
DR TreeFam; TF312822; -.
DR Reactome; R-RNO-8951664; Neddylation.
DR Reactome; R-RNO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q5M877; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000015125; Expressed in jejunum and 18 other tissues.
DR Genevisible; Q5M877; RN.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR CDD; cd03719; SOCS_SSB2; 1.
DR Gene3D; 2.60.120.920; -; 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001496; SOCS_box.
DR InterPro; IPR036036; SOCS_box-like_dom_sf.
DR InterPro; IPR003877; SPRY_dom.
DR InterPro; IPR037340; SSB2_SOCS.
DR Pfam; PF07525; SOCS_box; 1.
DR Pfam; PF00622; SPRY; 1.
DR SMART; SM00253; SOCS; 1.
DR SMART; SM00969; SOCS_box; 1.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF158235; SSF158235; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS50188; B302_SPRY; 1.
DR PROSITE; PS50225; SOCS; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..264
FT /note="SPRY domain-containing SOCS box protein 2"
FT /id="PRO_0000238476"
FT DOMAIN 26..221
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT DOMAIN 222..264
FT /note="SOCS box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..20
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 264 AA; 28797 MW; 2C3578010FE81C02 CRC64;
MGQTALARGS SSTPSSHALY SDLSPPEGLE ELLSAPPPDL GAQRHHGWNP KDCSENIDVK
EGGLCFERRP VAQSTDGVRG KRGYSRGLHA WEISWPLEQR GTHAVVGVAT ALAPLQADHY
AALLGSNSES WGWDIGRGKL YHQSKGLEAP QYPAGPQGEQ LVVPERLLVV LDMEEGTLGY
SIGGTYLGPA FRGLKGRTLY PSVSAVWGQC QVRIRYLGER RAEEPQSLLH LSRLCVRHAL
GDTRLGQISS LPLPPAMKRY LLYK
