SPSB3_HUMAN
ID SPSB3_HUMAN Reviewed; 355 AA.
AC Q6PJ21; D3DU78; Q49A96; Q86X18; Q8WXK5; Q96IE6; Q96RY2;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=SPRY domain-containing SOCS box protein 3;
DE Short=SSB-3;
GN Name=SPSB3; Synonyms=C16orf31, SSB3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Friedel E.J., Nicholson S.E., Nicola N.A., Kile B.T., Hilton D.J.;
RT "SOCS box proteins.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11157797; DOI=10.1093/hmg/10.4.339;
RA Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C.,
RA Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R.;
RT "Sequence, structure and pathology of the fully annotated terminal 2 Mb of
RT the short arm of human chromosome 16.";
RL Hum. Mol. Genet. 10:339-352(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, Hypothalamus, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH MET.
RX PubMed=15713673; DOI=10.1074/jbc.m413897200;
RA Wang D., Li Z., Messing E.M., Wu G.;
RT "The SPRY domain-containing SOCS box protein 1 (SSB-1) interacts with MET
RT and enhances the hepatocyte growth factor-induced Erk-Elk-1-serum response
RT element pathway.";
RL J. Biol. Chem. 280:16393-16401(2005).
CC -!- FUNCTION: May be a substrate recognition component of a SCF-like ECS
CC (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase
CC complex which mediates the ubiquitination and subsequent proteasomal
CC degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Interacts with MET. {ECO:0000269|PubMed:15713673}.
CC -!- INTERACTION:
CC Q6PJ21; Q9H0I2: ENKD1; NbExp=6; IntAct=EBI-3937206, EBI-744099;
CC Q6PJ21; P28799: GRN; NbExp=3; IntAct=EBI-3937206, EBI-747754;
CC Q6PJ21; Q9Y6R0: NUMBL; NbExp=5; IntAct=EBI-3937206, EBI-945925;
CC Q6PJ21; Q96N95-3: ZNF396; NbExp=3; IntAct=EBI-3937206, EBI-12328453;
CC Q6PJ21; B7Z5W0; NbExp=3; IntAct=EBI-3937206, EBI-10253951;
CC -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC BC complex, an adapter module in different E3 ubiquitin ligase
CC complexes. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SPSB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH41897.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=AAK61293.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF403028; AAL57347.1; -; mRNA.
DR EMBL; AE006639; AAK61293.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CH471112; EAW85619.1; -; Genomic_DNA.
DR EMBL; CH471112; EAW85620.1; -; Genomic_DNA.
DR EMBL; BC007588; AAH07588.2; -; mRNA.
DR EMBL; BC024244; AAH24244.1; -; mRNA.
DR EMBL; BC041897; AAH41897.1; ALT_SEQ; mRNA.
DR EMBL; BC047441; AAH47441.2; -; mRNA.
DR CCDS; CCDS32365.1; -.
DR RefSeq; NP_001311010.1; NM_001324081.1.
DR RefSeq; NP_543137.2; NM_080861.3.
DR AlphaFoldDB; Q6PJ21; -.
DR SMR; Q6PJ21; -.
DR BioGRID; 124775; 51.
DR IntAct; Q6PJ21; 18.
DR STRING; 9606.ENSP00000457206; -.
DR iPTMnet; Q6PJ21; -.
DR PhosphoSitePlus; Q6PJ21; -.
DR BioMuta; SPSB3; -.
DR DMDM; 126253805; -.
DR EPD; Q6PJ21; -.
DR MassIVE; Q6PJ21; -.
DR MaxQB; Q6PJ21; -.
DR PaxDb; Q6PJ21; -.
DR PeptideAtlas; Q6PJ21; -.
DR PRIDE; Q6PJ21; -.
DR ProteomicsDB; 67190; -.
DR Antibodypedia; 23246; 34 antibodies from 16 providers.
DR DNASU; 90864; -.
DR Ensembl; ENST00000301717.8; ENSP00000301717.4; ENSG00000162032.16.
DR Ensembl; ENST00000566339.6; ENSP00000457206.1; ENSG00000162032.16.
DR GeneID; 90864; -.
DR KEGG; hsa:90864; -.
DR MANE-Select; ENST00000566339.6; ENSP00000457206.1; NM_080861.4; NP_543137.2.
DR UCSC; uc002cmu.4; human.
DR CTD; 90864; -.
DR DisGeNET; 90864; -.
DR GeneCards; SPSB3; -.
DR HGNC; HGNC:30629; SPSB3.
DR HPA; ENSG00000162032; Low tissue specificity.
DR MIM; 611659; gene.
DR neXtProt; NX_Q6PJ21; -.
DR OpenTargets; ENSG00000162032; -.
DR PharmGKB; PA142670873; -.
DR VEuPathDB; HostDB:ENSG00000162032; -.
DR eggNOG; KOG3953; Eukaryota.
DR GeneTree; ENSGT01030000234629; -.
DR HOGENOM; CLU_042284_0_0_1; -.
DR InParanoid; Q6PJ21; -.
DR OMA; VRDCRCG; -.
DR OrthoDB; 1269591at2759; -.
DR PhylomeDB; Q6PJ21; -.
DR TreeFam; TF312822; -.
DR PathwayCommons; Q6PJ21; -.
DR Reactome; R-HSA-8951664; Neddylation.
DR SignaLink; Q6PJ21; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 90864; 9 hits in 1078 CRISPR screens.
DR ChiTaRS; SPSB3; human.
DR GenomeRNAi; 90864; -.
DR Pharos; Q6PJ21; Tdark.
DR PRO; PR:Q6PJ21; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q6PJ21; protein.
DR Bgee; ENSG00000162032; Expressed in pancreatic ductal cell and 188 other tissues.
DR ExpressionAtlas; Q6PJ21; baseline and differential.
DR Genevisible; Q6PJ21; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd12876; SPRY_SOCS3; 1.
DR Gene3D; 2.60.120.920; -; 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001496; SOCS_box.
DR InterPro; IPR003877; SPRY_dom.
DR InterPro; IPR035754; SPRY_SPSB3.
DR Pfam; PF00622; SPRY; 1.
DR SMART; SM00969; SOCS_box; 1.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS50188; B302_SPRY; 1.
DR PROSITE; PS50225; SOCS; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..355
FT /note="SPRY domain-containing SOCS box protein 3"
FT /id="PRO_0000278778"
FT DOMAIN 85..274
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT DOMAIN 264..315
FT /note="SOCS box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
FT REGION 37..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 327..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 171
FT /note="S -> L (in dbSNP:rs35816944)"
FT /id="VAR_052034"
FT CONFLICT 211
FT /note="S -> P (in Ref. 4; AAH47441)"
FT /evidence="ECO:0000305"
FT CONFLICT 269
FT /note="R -> S (in Ref. 4; AAH47441)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="P -> H (in Ref. 4; AAH24244)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="P -> R (in Ref. 4; AAH47441)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 355 AA; 39376 MW; 56BFA7A1DA27E254 CRC64;
MARRPRNSRA WHFVLSAARR DADARAVALA GSTNWGYDSD GQHSDSDSDP EYSTLPPSIP
SAVPVTGESF CDCAGQSEAS FCSSLHSAHR GRDCRCGEED EYFDWVWDDL NKSSATLLSC
DNRKVSFHME YSCGTAAIRG TKELGEGQHF WEIKMTSPVY GTDMMVGIGT SDVDLDKYRH
TFCSLLGRDE DSWGLSYTGL LHHKGDKTSF SSRFGQGSII GVHLDTWHGT LTFFKNRKCI
GVAATKLQNK RFYPMVCSTA ARSSMKVTRS CASATSLQYL CCHRLRQLRP DSGDTLEGLP
LPPGLKQVLH NKLGWVLSMS CSRRKAPVSD PQAATSAHPS SREPRPCQRK RCRRT
