ID   SPSS2_METAR             Reviewed;         463 AA.
AC   Q0W486;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=O-phospho-L-seryl-tRNA:Cys-tRNA synthase 2 {ECO:0000255|HAMAP-Rule:MF_01675};
DE            EC=2.5.1.73 {ECO:0000255|HAMAP-Rule:MF_01675};
DE   AltName: Full=Sep-tRNA:Cys-tRNA synthase 2 {ECO:0000255|HAMAP-Rule:MF_01675};
DE            Short=SepCysS 2 {ECO:0000255|HAMAP-Rule:MF_01675};
GN   OrderedLocusNames=UNCMA_14250; ORFNames=RCIX1553;
OS   Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanocellales; Methanocellaceae; Methanocella.
OX   NCBI_TaxID=351160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22066 / NBRC 105507 / MRE50;
RX   PubMed=16857943; DOI=10.1126/science.1127062;
RA   Erkel C., Kube M., Reinhardt R., Liesack W.;
RT   "Genome of rice cluster I archaea -- the key methane producers in the rice
RT   rhizosphere.";
RL   Science 313:370-372(2006).
CC   -!- FUNCTION: Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-
CC       cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)). {ECO:0000255|HAMAP-Rule:MF_01675}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L-
CC         cysteinyl-tRNA(Cys) + phosphate; Xref=Rhea:RHEA:25686, Rhea:RHEA-
CC         COMP:9679, Rhea:RHEA-COMP:9719, ChEBI:CHEBI:15378, ChEBI:CHEBI:29919,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:78517, ChEBI:CHEBI:78551; EC=2.5.1.73;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01675};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01675};
CC   -!- SUBUNIT: Homodimer. Interacts with SepRS. {ECO:0000255|HAMAP-
CC       Rule:MF_01675}.
CC   -!- SIMILARITY: Belongs to the SepCysS family. {ECO:0000255|HAMAP-
CC       Rule:MF_01675}.
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DR   EMBL; AM114193; CAJ36807.1; -; Genomic_DNA.
DR   RefSeq; WP_012035753.1; NC_009464.1.
DR   AlphaFoldDB; Q0W486; -.
DR   SMR; Q0W486; -.
DR   STRING; 351160.RCIX1553; -.
DR   EnsemblBacteria; CAJ36807; CAJ36807; RCIX1553.
DR   GeneID; 5144618; -.
DR   KEGG; rci:RCIX1553; -.
DR   PATRIC; fig|351160.9.peg.1468; -.
DR   eggNOG; arCOG00091; Archaea.
DR   OMA; WKLNTYG; -.
DR   OrthoDB; 24071at2157; -.
DR   Proteomes; UP000000663; Chromosome.
DR   GO; GO:0043766; F:Sep-tRNA:Cys-tRNA synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   HAMAP; MF_01675; Sep_Cys_tRNA_synth; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   InterPro; IPR013375; Sep_Cys-tRNA_synth_arc.
DR   PANTHER; PTHR43586:SF3; PTHR43586:SF3; 1.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR02539; SepCysS; 1.
PE   3: Inferred from homology;
KW   Protein biosynthesis; Pyridoxal phosphate; Reference proteome; Transferase.
FT   CHAIN           1..463
FT                   /note="O-phospho-L-seryl-tRNA:Cys-tRNA synthase 2"
FT                   /id="PRO_0000359466"
FT   BINDING         154..155
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01675"
FT   BINDING         259
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01675"
FT   BINDING         282..284
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01675"
FT   MOD_RES         285
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01675"
SQ   SEQUENCE   463 AA;  51508 MW;  8580B962884962ED CRC64;
     MIQLSNPRIN KTFEALFALE DIREVFRQSL PTGLSSAEEQ ALKGKLAEIR KYLDEIEADG
     VGNGARQKYT GKIGSLDVRT REEEYINIHP IQAAGRLTPE ARKAIIAYGD GYSTCDYCRK
     PFRLDKITKP GIQDFHGDLA RWLNMDQARV VPGARRGFQA VASALVEKGD SVIVSALAHY
     TEFLAVEQAG GVVKEVPKNE QNLITADATA QKIEDVRRTT GKLPVLIMID HFDYMFANEH
     DVYGIAKVAK QYGIPFLYNG AYTVGIAPVD GKKIGADFVV GSGHKSMASV APSGVLATTD
     EFAAKVFRTT QMVGDVTNRK FGIKEVENMG CTLMGGTLLS MIASFPAVVK RSQPQNWEDE
     VRKSNYFLEQ FRRVQGSNVL SEWPRKHTLT KVDTTGSYNK VAETHKRRGY FFNDELTAKG
     IIGEFAGATK TWKLNTYGLS WEKTKYLSEA FLDIARKYDL PIN