ID   SPS_THEM4               Reviewed;         423 AA.
AC   A6LKE9;
DT   22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Probable sucrose-phosphate synthase {ECO:0000303|PubMed:25846332};
DE            Short=psTm {ECO:0000303|PubMed:25846332};
DE            EC=2.4.1.14;
GN   OrderedLocusNames=Tmel_0533;
OS   Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429).
OC   Bacteria; Thermotogae; Thermotogales; Fervidobacteriaceae; Thermosipho.
OX   NCBI_TaxID=391009;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 12029 / CIP 104789 / BI429;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C.,
RA   Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.;
RT   "Complete sequence of Thermosipho melanesiensis BI429.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROBABLE FUNCTION.
RC   STRAIN=DSM 12029 / CIP 104789 / BI429;
RX   PubMed=25846332; DOI=10.1007/s00253-015-6548-7;
RA   Diricks M., De Bruyn F., Van Daele P., Walmagh M., Desmet T.;
RT   "Identification of sucrose synthase in nonphotosynthetic bacteria and
RT   characterization of the recombinant enzymes.";
RL   Appl. Microbiol. Biotechnol. 99:8465-8474(2015).
CC   -!- FUNCTION: Plays a role in sucrose synthesis by catalyzing the first
CC       step of sucrose biosynthesis from UDP-glucose and fructose-6-phosphate.
CC       {ECO:0000305|PubMed:25846332}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-fructose 6-phosphate + UDP-alpha-D-glucose = H(+) +
CC         sucrose 6(F)-phosphate + UDP; Xref=Rhea:RHEA:22172,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57634, ChEBI:CHEBI:57723,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885; EC=2.4.1.14;
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 1 family. {ECO:0000305}.
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DR   EMBL; CP000716; ABR30400.1; -; Genomic_DNA.
DR   RefSeq; WP_012056761.1; NC_009616.1.
DR   AlphaFoldDB; A6LKE9; -.
DR   SMR; A6LKE9; -.
DR   STRING; 391009.Tmel_0533; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   EnsemblBacteria; ABR30400; ABR30400; Tmel_0533.
DR   KEGG; tme:Tmel_0533; -.
DR   eggNOG; COG0438; Bacteria.
DR   HOGENOM; CLU_009583_24_2_0; -.
DR   OMA; HTMAKVK; -.
DR   Proteomes; UP000001110; Chromosome.
DR   GO; GO:0016157; F:sucrose synthase activity; IEA:InterPro.
DR   GO; GO:0046524; F:sucrose-phosphate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005986; P:sucrose biosynthetic process; IEA:InterPro.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR044161; SPS.
DR   InterPro; IPR000368; Sucrose_synth.
DR   PANTHER; PTHR46039; PTHR46039; 2.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   Pfam; PF00862; Sucrose_synth; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..423
FT                   /note="Probable sucrose-phosphate synthase"
FT                   /id="PRO_0000442260"
SQ   SEQUENCE   423 AA;  49174 MW;  9F10E0577A9EF717 CRC64;
     MKIAFFNPQG NFDKKDSHLT EHPDFGGQLI YVKELAKAMG KMGNKVDIIT RKIIDKKWPE
     FSGDFDYYPD AENVRIVRIA FGGDKFLNKE RLWDFLGEYV KNIYRFYQKE GFPDFVTTHY
     GDGGIAGAMF KKLTHIPYSF TAHSLGAQKK DKFKNAKDAE ERYRFSIRIS AEKVAMKYAS
     FIVTSTQQEK EEQYSHNEYI DVYPEIKDKI FVIPPGVNTN IFYPDDTDEY KFSKLPIIVS
     SRLDPKKNIE FVIESFNKYL KDGFELIIVL RKKPEEYTGY ERQLIEKAKK AKGKFLVITS
     QKELAKLYNS AAKHRGIFAL TSHYEPFGLA IIEAMACKLP VISTRNGGPV EILDNGKYGH
     LVSTHEEFKE AALKIKDNYE KLSEESYKRV MEKYTWERCA KEYLNLIEKE NVILPEFFEK
     QMG