SPT16_CRYNJ
ID SPT16_CRYNJ Reviewed; 1035 AA.
AC P0CQ22; Q55VJ3; Q5KKP5;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=FACT complex subunit SPT16;
DE AltName: Full=Facilitates chromatin transcription complex subunit SPT16;
GN Name=SPT16; OrderedLocusNames=CNC02310;
OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS MYA-565) (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=214684;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEC21 / ATCC MYA-565;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Component of the FACT complex, a general chromatin factor
CC that acts to reorganize nucleosomes. The FACT complex is involved in
CC multiple processes that require DNA as a template such as mRNA
CC elongation, DNA replication and DNA repair. During transcription
CC elongation the FACT complex acts as a histone chaperone that both
CC destabilizes and restores nucleosomal structure. It facilitates the
CC passage of RNA polymerase II and transcription by promoting the
CC dissociation of one histone H2A-H2B dimer from the nucleosome, then
CC subsequently promotes the reestablishment of the nucleosome following
CC the passage of RNA polymerase II (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms a stable heterodimer with POB3. The SPT16-POB3 dimer
CC weakly associates with multiple molecules of NHP6 to form the FACT
CC complex (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase M24 family. SPT16 subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: Although related to the peptidase M24 family, this protein
CC lacks conserved active site residues suggesting that it may lack
CC peptidase activity. {ECO:0000305}.
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DR EMBL; AE017343; AAW42200.1; -; Genomic_DNA.
DR RefSeq; XP_569507.1; XM_569507.1.
DR AlphaFoldDB; P0CQ22; -.
DR SMR; P0CQ22; -.
DR STRING; 5207.AAW42200; -.
DR PaxDb; P0CQ22; -.
DR EnsemblFungi; AAW42200; AAW42200; CNC02310.
DR GeneID; 3256484; -.
DR KEGG; cne:CNC02310; -.
DR VEuPathDB; FungiDB:CNC02310; -.
DR eggNOG; KOG1189; Eukaryota.
DR HOGENOM; CLU_004627_1_0_1; -.
DR InParanoid; P0CQ22; -.
DR OMA; HQFFLDG; -.
DR OrthoDB; 145488at2759; -.
DR Proteomes; UP000002149; Chromosome 3.
DR GO; GO:0035101; C:FACT complex; IBA:GO_Central.
DR GO; GO:0031491; F:nucleosome binding; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0034724; P:DNA replication-independent chromatin organization; IBA:GO_Central.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 3.40.350.10; -; 1.
DR Gene3D; 3.90.230.10; -; 1.
DR InterPro; IPR029149; Creatin/AminoP/Spt16_NTD.
DR InterPro; IPR036005; Creatinase/aminopeptidase-like.
DR InterPro; IPR013719; DUF1747.
DR InterPro; IPR029148; FACT-Spt16_Nlobe.
DR InterPro; IPR013953; FACT_Spt16.
DR InterPro; IPR000994; Pept_M24.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR040258; Spt16.
DR PANTHER; PTHR13980; PTHR13980; 1.
DR Pfam; PF14826; FACT-Spt16_Nlob; 1.
DR Pfam; PF00557; Peptidase_M24; 1.
DR Pfam; PF08512; Rtt106; 1.
DR Pfam; PF08644; SPT16; 1.
DR SMART; SM01285; FACT-Spt16_Nlob; 1.
DR SMART; SM01287; Rtt106; 1.
DR SMART; SM01286; SPT16; 1.
DR SUPFAM; SSF55920; SSF55920; 1.
PE 3: Inferred from homology;
KW Chromosome; Coiled coil; DNA damage; DNA repair; DNA replication; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..1035
FT /note="FACT complex subunit SPT16"
FT /id="PRO_0000245183"
FT REGION 440..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 944..1035
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 637..657
FT /evidence="ECO:0000255"
FT COMPBIAS 949..982
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 999..1035
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1035 AA; 115812 MW; 8A2C2014A3816F3D CRC64;
MSDIRLDSAT FFKRAAKIFD SWEKPSGDTQ ALEDINSIAI ILGDPNDEVA SYTKTTALQL
WLLGYEFPST LMVFEKSPRK VTFVCGSSKA KLIRQLQPSD GIEIDVKVRS KDATAAKETM
EEVVASLNGK FGSLPKDRPI GKLVDEWNSA VESKGDLEVV DVAIPISAVL AEKDGEELKT
IITSAKLTST VMINYFKSKM ESIIDRGTKM SHEALAQLVE EKIGNEEKGP DMKLWNKNPS
LGEIDFASSE FVYSPVIQSG GKYDLKVTAA SNNDNLKPGI ILANMGIRYK NYCSNMGRTF
LISPSKKQET QYTTLLEVRK EALALLKTGA VASDVYNSVH QSLETKNATL ADSFLKNLGF
ATGMEYRDSS FLLNAKNNRE LKENMVLVLT IGVADLPDAK NKGKTYSLLL SDTVKIGQNG
AVVLTEGCTR LSDVVMDMEE EEEEDVKPQI DKKPKINNSP KKPRSSTVGG RVLNAKTRGA
NREQATQTTA EKIKTNQQRL HAQLNADGVK RWEADAGGKN GAQQKVVKRY ESYRREEQLP
RAVEDRRIYV DEQRQSVVLP INGYAVPYHI STIKNVTKTE ESNHMVLRIN FQSPGQIAGK
KEDMPFEDPD ANFIRSVSFR SQDQRHMLKV YEAITALKKA AVKRETERKE LADVIEQEKL
IEVKGRHPYV LKNVFPRPGP EGKKTDGNVE IHQNGIRFRP DGPASKIDIL FSNIKHLFFQ
PSEKELIVII HVHLKAPIML GKKKTSDVQF YREVADMSFD ETGGKKRRAR YGDEDEIEQE
QEDRKRRAEL DKLFHDFARR IETAAQAQQF ELEVDVPFRE LGFNGVPHKS IVALLPTTNC
LIHISELPFT VITLSEVEIV HLERVQFGLK NFDMVFVLQD LKKPPVHINS IPVAHLDNVK
EWLDSCDVPI SEGPVNLSWP AIMKTVNEDP HAFYAEGGWN FLTGSGSDDG SEESEEGSEF
EGDSDVFDES SGSDEDSESA FEGDSDSASA ESLSDEGEDW DELERKAKRA DEKHRTDRGG
DSDDDGKKKK KGSRR