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SPT16_DROME
ID   SPT16_DROME             Reviewed;        1083 AA.
AC   Q8IRG6; O17045; O17046; Q7YTY1; Q86SB9; Q9W071;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 2.
DT   03-AUG-2022, entry version 145.
DE   RecName: Full=FACT complex subunit spt16;
DE   AltName: Full=Facilitates chromatin transcription complex subunit SPT16;
DE   AltName: Full=dSPT16;
GN   Name=dre4; Synonyms=spt16; ORFNames=CG1828;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RA   Washington A.V., Robinson P., Sliter T.J.;
RL   Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC   STRAIN=Berkeley; TISSUE=Testis;
RA   Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA   Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA   Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA   Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA   Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 40-1083 (ISOFORM B), PROTEIN SEQUENCE OF
RP   134-410; 613-627; 670-678; 703-710 AND 760-771, FUNCTION, AND INTERACTION
RP   WITH SSRP AND TRL.
RX   PubMed=12815073; DOI=10.1101/gad.1086803;
RA   Shimojima T., Okada M., Nakayama T., Ueda H., Okawa K., Iwamatsu A.,
RA   Handa H., Hirose S.;
RT   "Drosophila FACT contributes to Hox gene expression through physical and
RT   functional interactions with GAGA factor.";
RL   Genes Dev. 17:1605-1616(2003).
RN   [6]
RP   PRELIMINARY FUNCTION.
RX   PubMed=1551577; DOI=10.1093/genetics/130.3.555;
RA   Sliter T.J., Gilbert L.I.;
RT   "Developmental arrest and ecdysteroid deficiency resulting from mutations
RT   at the dre4 locus of Drosophila.";
RL   Genetics 130:555-568(1992).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=12934007; DOI=10.1126/science.1085712;
RA   Saunders A., Werner J., Andrulis E.D., Nakayama T., Hirose S., Reinberg D.,
RA   Lis J.T.;
RT   "Tracking FACT and the RNA polymerase II elongation complex through
RT   chromatin in vivo.";
RL   Science 301:1094-1096(2003).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-437, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   TISSUE=Embryo;
RX   PubMed=18327897; DOI=10.1021/pr700696a;
RA   Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT   "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL   J. Proteome Res. 7:1675-1682(2008).
CC   -!- FUNCTION: Component of the FACT complex, a general chromatin factor
CC       that acts to reorganize nucleosomes. The FACT complex is involved in
CC       multiple processes that require DNA as a template such as mRNA
CC       elongation, DNA replication and DNA repair. During transcription
CC       elongation the FACT complex acts as a histone chaperone that both
CC       destabilizes and restores nucleosomal structure. It facilitates the
CC       passage of RNA polymerase II and transcription by promoting the
CC       dissociation of one histone H2A-H2B dimer from the nucleosome, then
CC       subsequently promotes the reestablishment of the nucleosome following
CC       the passage of RNA polymerase II. The FACT complex is required for
CC       expression of Hox genes. {ECO:0000269|PubMed:12815073}.
CC   -!- SUBUNIT: Component of the FACT complex, a stable heterodimer of
CC       dre4/spt16 and Ssrp. Interacts with TRL/GAGA.
CC       {ECO:0000269|PubMed:12815073}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12934007}. Chromosome
CC       {ECO:0000269|PubMed:12934007}. Note=Colocalizes with RNA polymerase II
CC       on chromatin. Recruited to actively transcribed loci.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=B;
CC         IsoId=Q8IRG6-1; Sequence=Displayed;
CC       Name=A;
CC         IsoId=Q8IRG6-2; Sequence=VSP_019625;
CC   -!- SIMILARITY: Belongs to the peptidase M24 family. SPT16 subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Although related to the peptidase M24 family, this protein
CC       lacks conserved active site residues suggesting that it may lack
CC       peptidase activity. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB80935.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=AAB80936.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF023269; AAB80935.1; ALT_FRAME; mRNA.
DR   EMBL; AF023270; AAB80936.1; ALT_FRAME; mRNA.
DR   EMBL; AE014296; AAF47587.2; -; Genomic_DNA.
DR   EMBL; AE014296; AAN11502.2; -; Genomic_DNA.
DR   EMBL; BT010116; AAQ22585.1; -; mRNA.
DR   EMBL; AB083008; BAC54898.1; -; mRNA.
DR   PIR; T13928; T13928.
DR   PIR; T13929; T13929.
DR   RefSeq; NP_476610.2; NM_057262.4. [Q8IRG6-2]
DR   RefSeq; NP_728686.2; NM_167922.3. [Q8IRG6-1]
DR   AlphaFoldDB; Q8IRG6; -.
DR   SMR; Q8IRG6; -.
DR   BioGRID; 63776; 8.
DR   IntAct; Q8IRG6; 9.
DR   STRING; 7227.FBpp0072743; -.
DR   MEROPS; M24.974; -.
DR   iPTMnet; Q8IRG6; -.
DR   PaxDb; Q8IRG6; -.
DR   PRIDE; Q8IRG6; -.
DR   DNASU; 38248; -.
DR   EnsemblMetazoa; FBtr0072864; FBpp0072743; FBgn0002183. [Q8IRG6-2]
DR   EnsemblMetazoa; FBtr0072865; FBpp0072744; FBgn0002183. [Q8IRG6-1]
DR   GeneID; 38248; -.
DR   KEGG; dme:Dmel_CG1828; -.
DR   CTD; 38248; -.
DR   FlyBase; FBgn0002183; dre4.
DR   VEuPathDB; VectorBase:FBgn0002183; -.
DR   eggNOG; KOG1189; Eukaryota.
DR   GeneTree; ENSGT00390000014495; -.
DR   HOGENOM; CLU_004627_0_0_1; -.
DR   InParanoid; Q8IRG6; -.
DR   OMA; HQFFLDG; -.
DR   PhylomeDB; Q8IRG6; -.
DR   Reactome; R-DME-112382; Formation of RNA Pol II elongation complex.
DR   Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
DR   Reactome; R-DME-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR   Reactome; R-DME-6804756; Regulation of TP53 Activity through Phosphorylation.
DR   Reactome; R-DME-75955; RNA Polymerase II Transcription Elongation.
DR   SignaLink; Q8IRG6; -.
DR   BioGRID-ORCS; 38248; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 38248; -.
DR   PRO; PR:Q8IRG6; -.
DR   Proteomes; UP000000803; Chromosome 3L.
DR   Bgee; FBgn0002183; Expressed in oocyte and 25 other tissues.
DR   ExpressionAtlas; Q8IRG6; baseline and differential.
DR   Genevisible; Q8IRG6; DM.
DR   GO; GO:0005829; C:cytosol; IDA:CAFA.
DR   GO; GO:0035101; C:FACT complex; IDA:FlyBase.
DR   GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR   GO; GO:0005703; C:polytene chromosome puff; IDA:UniProtKB.
DR   GO; GO:0003682; F:chromatin binding; IDA:FlyBase.
DR   GO; GO:0031491; F:nucleosome binding; IDA:FlyBase.
DR   GO; GO:0046982; F:protein heterodimerization activity; IPI:CAFA.
DR   GO; GO:0047485; F:protein N-terminus binding; IMP:CAFA.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0034724; P:DNA replication-independent chromatin organization; IBA:GO_Central.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:FlyBase.
DR   GO; GO:1902275; P:regulation of chromatin organization; IMP:FlyBase.
DR   GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   CDD; cd01091; CDC68-like; 1.
DR   DisProt; DP00721; -.
DR   Gene3D; 2.30.29.30; -; 1.
DR   Gene3D; 3.40.350.10; -; 1.
DR   Gene3D; 3.90.230.10; -; 1.
DR   IDEAL; IID50095; -.
DR   InterPro; IPR029149; Creatin/AminoP/Spt16_NTD.
DR   InterPro; IPR036005; Creatinase/aminopeptidase-like.
DR   InterPro; IPR013719; DUF1747.
DR   InterPro; IPR029148; FACT-Spt16_Nlobe.
DR   InterPro; IPR013953; FACT_Spt16.
DR   InterPro; IPR000994; Pept_M24.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR040258; Spt16.
DR   InterPro; IPR033825; Spt16_M24.
DR   PANTHER; PTHR13980; PTHR13980; 1.
DR   Pfam; PF14826; FACT-Spt16_Nlob; 1.
DR   Pfam; PF00557; Peptidase_M24; 1.
DR   Pfam; PF08512; Rtt106; 1.
DR   Pfam; PF08644; SPT16; 1.
DR   SMART; SM01285; FACT-Spt16_Nlob; 1.
DR   SMART; SM01287; Rtt106; 1.
DR   SMART; SM01286; SPT16; 1.
DR   SUPFAM; SSF55920; SSF55920; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Chromosome; Coiled coil; Direct protein sequencing;
KW   DNA damage; DNA repair; DNA replication; Nucleus; Phosphoprotein;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..1083
FT                   /note="FACT complex subunit spt16"
FT                   /id="PRO_0000245174"
FT   REGION          923..1083
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          466..504
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        938..988
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        989..1022
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1032..1076
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         437
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   VAR_SEQ         1031
FT                   /note="H -> HSRRDREEARSSSHSKKHKSNSSSSSSHLKSSSSKHGSSS (in
FT                   isoform A)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_019625"
FT   CONFLICT        345
FT                   /note="L -> V (in Ref. 1; AAB80935/AAB80936)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        598
FT                   /note="A -> R (in Ref. 1; AAB80935/AAB80936)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1057
FT                   /note="S -> I (in Ref. 1; AAB80935/AAB80936)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1080..1081
FT                   /note="KS -> NA (in Ref. 1; AAB80935/AAB80936)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1083 AA;  123583 MW;  6A2AF4012D93D449 CRC64;
     MSSFVLDKEA FVRRVKRLYT EWRAPSIGHD DALRNLDCIM SIVGVEEDVM YSKSMALQLW
     LLGYELTDTI SVFCSDAVYF LTSKKKIEFL KQTQNITEEG FPEINLLVRD RTDKDQGNFE
     KLIKALQNSK KGKRLGVFAK DAYPGEFSEA WKKSLTASKF EHVDISTIIA YLMCPKDESE
     INNIRKASLV SMDIFNKYLK DEIMDIIDSD RKVKHNKLSD GCEAAIGEKK YTSGLDPRLL
     DMAYPPIIQS GGAYSLKFSA VADKNPLHFG VIVCSLGARY KSYCSNISRT FLVNPTEAMQ
     ENYTFLVSVQ EEILKLLVPG TKLCDVYEKT LDFVKKEKPS MVDNLPKSFG FAMGLEFREN
     SIVIGPKCQA LLKKNMVFNL HVGISNLTNP EATDKEGKNY ALFIGDTVLV GEQSPASVMT
     PSKKKIKNVG IFIKDDSDEE DVDDKKTAKE DQGTEILGRS KRNAVLESKL RNEINTEEKR
     KEHQRELAQQ LNERAKDRLA RQGNSKEVEK VRKNTVSYKS ISQMPREPEV KELKLYVDKK
     YETVIMPVFG IQVPFHISTI KNISQSVEGE YTYLRINFFH PGATMGRNEG GLYPQPEATF
     VKEVTYRSSN VKEHGEVGAP SANLNNAFRL IKEVQKRFKT REAEEREKED LVKQDTLILS
     QNKGNPKLKD LYIRPNIVTK RMTGSLEAHS NGFRYISVRG DKVDILYNNI KSAFFQPCDG
     EMIILLHFHL KYAIMFGKKK HVDVQFYTEV GEITTDLGKH QHMHDRDDLA AEQAERELRH
     KLKTAFKSFC EKVETMTKSV VEFDTPFREL GFPGAPFRST VTLQPTSGSL VNLTEWPPFV
     ITLDDVELVH FERVQFHLRN FDMIFVFKEY NKKVAMVNAI PMNMLDHVKE WLNSCDIRYS
     EGVQSLNWQK IMKTITDDPE GFFEQGGWTF LDPESGSEGE NETAESEEDE AYNPTDAESD
     EESDEDSEYS EASEDSEESD EDLGSDEESG KDWSDLEREA AEEDRNHDYA ADDKPRNGKF
     DSKKHGKSSK HSPSKSSKDK YNSRDKHHSS SSSGNKSSSK DKDRKRSRDD SRDNGHKSKK
     SRH
 
 
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