SPT16_MOUSE
ID SPT16_MOUSE Reviewed; 571 AA.
AC Q8C636; Q9D5W9;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Spermatogenesis-associated protein 16;
GN Name=Spata16;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=12529416; DOI=10.1093/molehr/gag005;
RA Xu M., Xiao J., Chen J., Li J., Yin L., Zhu H., Zhou Z., Sha J.;
RT "Identification and characterization of a novel human testis-specific Golgi
RT protein, NYD-SP12.";
RL Mol. Hum. Reprod. 9:9-17(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16372119; DOI=10.1111/j.1745-7262.2006.00089.x;
RA Lu L., Lin M., Xu M., Zhou Z.-M., Sha J.;
RT "Gene functional research using polyethylenimine-mediated in vivo gene
RT transfection into mouse spermatogenic cells.";
RL Asian J. Androl. 8:53-59(2006).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP ARG-284.
RX PubMed=29065458; DOI=10.3390/ijms18102208;
RA Fujihara Y., Oji A., Larasati T., Kojima-Kita K., Ikawa M.;
RT "Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation
RT Revealed by CRISPR/Cas9-Mediated Mouse Models.";
RL Int. J. Mol. Sci. 18:0-0(2017).
CC -!- FUNCTION: Essential for spermiogenesis and male fertility
CC (PubMed:29065458). Involved in the formation of acrosome during
CC spermatogenesis (PubMed:16372119). {ECO:0000269|PubMed:16372119,
CC ECO:0000269|PubMed:29065458}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000269|PubMed:12529416,
CC ECO:0000269|PubMed:16372119}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000269|PubMed:16372119}. Note=Shift from Golgi to sperm
CC acrosome. {ECO:0000269|PubMed:16372119}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8C636-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C636-2; Sequence=VSP_030726;
CC -!- TISSUE SPECIFICITY: Testis-specific. Expressed in spermatocytes and
CC round and elongated spermatids in the seminiferous tubules.
CC {ECO:0000269|PubMed:12529416, ECO:0000269|PubMed:16372119,
CC ECO:0000269|PubMed:29065458}.
CC -!- DISRUPTION PHENOTYPE: Male mice are infertile with spermiogenic arrest
CC and impaired differentiation of round spermatids into the mature
CC spermatozoa. {ECO:0000269|PubMed:29065458}.
CC -!- SIMILARITY: Belongs to the SPATA16 family. {ECO:0000305}.
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DR EMBL; AK076628; BAC36421.1; -; mRNA.
DR EMBL; AK014869; BAB29592.1; -; mRNA.
DR EMBL; BC100443; AAI00444.1; -; mRNA.
DR EMBL; BC132205; AAI32206.1; -; mRNA.
DR EMBL; BC132539; AAI32540.1; -; mRNA.
DR CCDS; CCDS17269.1; -. [Q8C636-1]
DR CCDS; CCDS38403.1; -. [Q8C636-2]
DR RefSeq; NP_081859.1; NM_027583.3. [Q8C636-2]
DR RefSeq; NP_083426.1; NM_029150.4. [Q8C636-1]
DR AlphaFoldDB; Q8C636; -.
DR SMR; Q8C636; -.
DR STRING; 10090.ENSMUSP00000043378; -.
DR iPTMnet; Q8C636; -.
DR PhosphoSitePlus; Q8C636; -.
DR PaxDb; Q8C636; -.
DR PRIDE; Q8C636; -.
DR ProteomicsDB; 263331; -. [Q8C636-1]
DR ProteomicsDB; 263332; -. [Q8C636-2]
DR Antibodypedia; 46805; 92 antibodies from 18 providers.
DR DNASU; 70862; -.
DR Ensembl; ENSMUST00000047005; ENSMUSP00000043378; ENSMUSG00000039335. [Q8C636-1]
DR Ensembl; ENSMUST00000108305; ENSMUSP00000103941; ENSMUSG00000039335. [Q8C636-2]
DR GeneID; 70862; -.
DR KEGG; mmu:70862; -.
DR UCSC; uc008otf.2; mouse. [Q8C636-2]
DR UCSC; uc008otg.1; mouse. [Q8C636-1]
DR CTD; 83893; -.
DR MGI; MGI:1918112; Spata16.
DR VEuPathDB; HostDB:ENSMUSG00000039335; -.
DR eggNOG; ENOG502RSQ1; Eukaryota.
DR GeneTree; ENSGT00390000015332; -.
DR HOGENOM; CLU_034262_0_0_1; -.
DR InParanoid; Q8C636; -.
DR OMA; RRQKMKM; -.
DR OrthoDB; 548331at2759; -.
DR TreeFam; TF335624; -.
DR BioGRID-ORCS; 70862; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Spata16; mouse.
DR PRO; PR:Q8C636; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8C636; protein.
DR Bgee; ENSMUSG00000039335; Expressed in spermatid and 3 other tissues.
DR Genevisible; Q8C636; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0007286; P:spermatid development; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR029161; SPATA16.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR47228; PTHR47228; 1.
DR Pfam; PF15015; NYD-SP12_N; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Developmental protein;
KW Differentiation; Golgi apparatus; Reference proteome; Spermatogenesis.
FT CHAIN 1..571
FT /note="Spermatogenesis-associated protein 16"
FT /id="PRO_0000315832"
FT REGION 30..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 533..571
FT /note="GRIEDFLYQLEDSFLKTKKLRTARRQKTKMKRLQTVQQN -> KF (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_030726"
FT MUTAGEN 284
FT /note="R->Q: No loss of male fertility."
FT /evidence="ECO:0000269|PubMed:29065458"
SQ SEQUENCE 571 AA; 65006 MW; 0B8DF528389EF26E CRC64;
MDSGKSRSLE TTVKRVYRDL LLPKINTSKK MSTLTNSPSS LEGTPGIKKN SGEMQAEASA
ERVKLTKSIK EKQNNELEKV ALKRKADSEE KLVGKKEAKI MELDNQLVTT VPLPHIPLKN
IMDVEMKLVY VDEEDVSYEF AQPNMCLGLQ ATGQTATTMP PASPRDLTTL PQIDKWLQVA
LKDASSCYRQ KKYAVAAGQF RTALELCSKG AALGKPFEAH AEDIASIASF IETKLVTCYL
RMRKPDLALN HAHRSIVLNP AYFRNHLRQA AVFRCLERYS EAARSAMIAD YMFWLCGGSE
HSVSKLIKLY WQAMIEEAIT RAEAFSVMYT PFATRIKPEN IEKVKEVFMR THPTYVDCIY
TDTQGLHVLP QTADWSCFPP QQYLLTLGFK NKEDGKFLEK VSSRKLPTYT EHKTPFSPLT
REDTVRHMET VGKRILPILD FIRSTQLNGN FHACSGVMEK LHYASLLSRL QRVKEQAQVI
NQAMAELATV PYLQDISQQE AELLQSLMAD AMDTLEGKKS DKERVWNTIQ KVGRIEDFLY
QLEDSFLKTK KLRTARRQKT KMKRLQTVQQ N
