ID   SPT16_ORYSJ             Reviewed;        1056 AA.
AC   Q7X923; Q0JE60;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 2.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=FACT complex subunit SPT16;
DE   AltName: Full=Facilitates chromatin transcription complex subunit SPT16;
GN   Name=SPT16; OrderedLocusNames=Os04g0321600, LOC_Os04g25550;
GN   ORFNames=OSJNBb0062H02.2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Component of the FACT complex, a general chromatin factor
CC       that acts to reorganize nucleosomes. The FACT complex is involved in
CC       multiple processes that require DNA as a template such as mRNA
CC       elongation, DNA replication and DNA repair. During transcription
CC       elongation the FACT complex acts as a histone chaperone that both
CC       destabilizes and restores nucleosomal structure. It facilitates the
CC       passage of RNA polymerase II and transcription by promoting the
CC       dissociation of one histone H2A-H2B dimer from the nucleosome, then
CC       subsequently promotes the reestablishment of the nucleosome following
CC       the passage of RNA polymerase II. SSRP1 binds specifically to double-
CC       stranded DNA (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the FACT complex, a stable heterodimer of SPT16
CC       and SSRP. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M24 family. SPT16 subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Although related to the peptidase M24 family, this protein
CC       lacks conserved active site residues suggesting that it may lack
CC       peptidase activity. {ECO:0000305}.
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DR   EMBL; AL663005; CAD40293.2; -; Genomic_DNA.
DR   EMBL; AP008210; BAF14377.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS88578.1; -; Genomic_DNA.
DR   EMBL; AK121570; BAH00556.1; -; mRNA.
DR   RefSeq; XP_015635636.1; XM_015780150.1.
DR   AlphaFoldDB; Q7X923; -.
DR   SMR; Q7X923; -.
DR   STRING; 4530.OS04T0321600-01; -.
DR   PaxDb; Q7X923; -.
DR   PRIDE; Q7X923; -.
DR   EnsemblPlants; Os04t0321600-01; Os04t0321600-01; Os04g0321600.
DR   GeneID; 4335478; -.
DR   Gramene; Os04t0321600-01; Os04t0321600-01; Os04g0321600.
DR   KEGG; osa:4335478; -.
DR   eggNOG; KOG1189; Eukaryota.
DR   HOGENOM; CLU_004627_1_0_1; -.
DR   InParanoid; Q7X923; -.
DR   OMA; HQFFLDG; -.
DR   OrthoDB; 145488at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q7X923; OS.
DR   GO; GO:0035101; C:FACT complex; IBA:GO_Central.
DR   GO; GO:0031491; F:nucleosome binding; IBA:GO_Central.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0034724; P:DNA replication-independent chromatin organization; IBA:GO_Central.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   CDD; cd01091; CDC68-like; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   Gene3D; 3.40.350.10; -; 1.
DR   Gene3D; 3.90.230.10; -; 1.
DR   InterPro; IPR029149; Creatin/AminoP/Spt16_NTD.
DR   InterPro; IPR036005; Creatinase/aminopeptidase-like.
DR   InterPro; IPR013719; DUF1747.
DR   InterPro; IPR029148; FACT-Spt16_Nlobe.
DR   InterPro; IPR013953; FACT_Spt16.
DR   InterPro; IPR000994; Pept_M24.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR033825; Spt16_M24.
DR   Pfam; PF14826; FACT-Spt16_Nlob; 1.
DR   Pfam; PF00557; Peptidase_M24; 1.
DR   Pfam; PF08512; Rtt106; 1.
DR   Pfam; PF08644; SPT16; 1.
DR   SMART; SM01285; FACT-Spt16_Nlob; 1.
DR   SMART; SM01287; Rtt106; 1.
DR   SMART; SM01286; SPT16; 1.
DR   SUPFAM; SSF55920; SSF55920; 1.
PE   2: Evidence at transcript level;
KW   Chromosome; Coiled coil; DNA damage; DNA repair; DNA replication; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..1056
FT                   /note="FACT complex subunit SPT16"
FT                   /id="PRO_0000245177"
FT   REGION          467..524
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          942..1056
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          316..336
FT                   /evidence="ECO:0000255"
FT   COILED          768..789
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        477..503
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        942..998
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        999..1029
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1056 AA;  118588 MW;  AB2264E925569702 CRC64;
     MADNGNAKPG GGGSGAYTIN LDNFSKRLKV FYDHWKEHNS DLWGSSNAIA IATPPPSEDL
     RYLKSSALDV WLLGYEFPET IIVFMHKQIH FLCSQKKANL IGTLKKAAND AVGADIVLHV
     KAKNDSGVGL MEDIVRAVCA QSKSDDPIVG HIAKEAPEGK LLEAWADKLS SSSVQLTDIT
     NGFSELFAMK DTSEITCVKK ASYLTSSVMK NFVVPKLEKV IDEERKVTHS SLMDETEKAI
     LDPLKVKVKL KAENVDICYP PVFQSGGKFD LKPGASSNDD YLYYDSASVI ICAIGARYGN
     YCSNMARTFL IDATPTQIKA YETLMKAHEA ALEALKPGNR MSAVYQAAVD VIEKNAPELL
     RNLTKSAGTG IGLEFRESGL NLNPKNDRII KAGMVFNVSL GLHNLQAEKK SEKTKQYSLL
     LADTCLVPLE NLTASCSKLV KDVAYSFNDE DEVLPVKKVE VNAKEALPPT KATLRSDNQE
     MSKEELRRQH QAELARQKNE ETARRLAGVG SGSGDGRGPS RSSNELVAYK NVNDVPYARE
     LVIQVDQKNE AVLLPIYGSM VPFHVSTVKS VTSHQDNRTC TIRIFFNVPG MPFSNDSNLK
     SQGAIYLKEI TFRSKDPRHS SEVVQQIKTL RRQVASRESE RAERATLVTQ EKLQLTSNRN
     KPVRLSDVWI RPAFGGRGRK LTGTLESHVN GFRYSTSRAD ERVDIMYGNV KHAFFQPAEK
     EMITLLHFHL HNHIMVGNKK TKDVQFYVEV MDVVQTLGGN RRSALDPDEI EEEQRERDRK
     NRINMDFQNF VNKVNDHWSQ PQFKGLDLEF DVPLRELGFH GVPYKASAFI IPTSTCLVEL
     IETPFLVVTL SEIEIVNLER VGFGTKNFDM AIVFKDFKKD VLRIDSIPST SLDAIKEWLD
     TTDLKYYESR LNLNWRPILK TIIDDPQKFI DDGGWEFLNM EASDSETEET EESDQGYEPS
     DAEPESESED EDSDSESLVE SDEDDEDDSE EDSEEEKGKT WEELEREASN ADRENGAESD
     SEEERRRRKV KTFSKSRPPP ERSSFKGGPS KKPKFR