SPT19_MOUSE
ID SPT19_MOUSE Reviewed; 154 AA.
AC Q9DAQ9; Q810M9;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Spermatogenesis-associated protein 19, mitochondrial;
DE AltName: Full=Spermatogenic cell-specific gene 1 protein;
DE Short=Spergen-1;
DE Flags: Precursor;
GN Name=Spata19; Synonyms=Spergen1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=15139970; DOI=10.1111/j.1365-2605.2004.00466.x;
RA Matsuoka Y., Iguchi N., Kitamura K., Nishimura H., Manabe H., Miyagawa Y.,
RA Koga M., Matsumiya K., Okuyama A., Tanaka H., Nishimune Y.;
RT "Cloning and characterization of a mouse spergen-1 localized in sperm
RT mitochondria.";
RL Int. J. Androl. 27:152-160(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=26265198; DOI=10.1002/mrd.22536;
RA Mi Y., Shi Z., Li J.;
RT "Spata19 is critical for sperm mitochondrial function and male fertility.";
RL Mol. Reprod. Dev. 82:907-913(2015).
CC -!- FUNCTION: Essential for sperm motility and male fertility
CC (PubMed:26265198). Plays an important role in sperm motility by
CC regulating the organization and function of the mitochondria and is
CC also required for correct sperm midpiece assembly (PubMed:26265198).
CC {ECO:0000269|PubMed:26265198}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:15139970}. Mitochondrion
CC {ECO:0000269|PubMed:15139970}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:26265198}. Note=Localizes to the midpiece of the
CC sperm flagellum. {ECO:0000269|PubMed:26265198}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9DAQ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9DAQ9-2; Sequence=VSP_020764, VSP_020765;
CC -!- TISSUE SPECIFICITY: Expressed specifically in adult testis (at protein
CC level). {ECO:0000269|PubMed:15139970, ECO:0000269|PubMed:26265198}.
CC -!- DISRUPTION PHENOTYPE: Male mice are infertile, their sperms exhibit
CC disorganized mitochondrial structure and the sperm-abundance of
CC mitochondrial proteins and activities of mitochondrial respiratory
CC chain complex IV, and ATP levels are significantly reduced
CC (PubMed:26265198). Epididymal sperm possess seriously disrupted
CC midpieces, leading to a severe reduction in sperm motility and
CC infertility (PubMed:26265198). {ECO:0000269|PubMed:26265198}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB120716; BAD01115.1; -; mRNA.
DR EMBL; AK005610; BAB24149.1; -; mRNA.
DR EMBL; BC049742; AAH49742.1; -; mRNA.
DR CCDS; CCDS22941.1; -. [Q9DAQ9-1]
DR CCDS; CCDS90524.1; -. [Q9DAQ9-2]
DR RefSeq; NP_001291987.1; NM_001305058.1. [Q9DAQ9-2]
DR RefSeq; NP_083575.1; NM_029299.3. [Q9DAQ9-1]
DR AlphaFoldDB; Q9DAQ9; -.
DR STRING; 10090.ENSMUSP00000034473; -.
DR PhosphoSitePlus; Q9DAQ9; -.
DR PaxDb; Q9DAQ9; -.
DR PRIDE; Q9DAQ9; -.
DR ProteomicsDB; 257385; -. [Q9DAQ9-1]
DR ProteomicsDB; 257386; -. [Q9DAQ9-2]
DR Antibodypedia; 46141; 122 antibodies from 25 providers.
DR DNASU; 75469; -.
DR Ensembl; ENSMUST00000034473; ENSMUSP00000034473; ENSMUSG00000031991. [Q9DAQ9-1]
DR Ensembl; ENSMUST00000214287; ENSMUSP00000149525; ENSMUSG00000031991. [Q9DAQ9-2]
DR GeneID; 75469; -.
DR KEGG; mmu:75469; -.
DR UCSC; uc009oqo.2; mouse. [Q9DAQ9-1]
DR UCSC; uc057aob.1; mouse. [Q9DAQ9-2]
DR CTD; 219938; -.
DR MGI; MGI:1922719; Spata19.
DR VEuPathDB; HostDB:ENSMUSG00000031991; -.
DR eggNOG; ENOG502SAUZ; Eukaryota.
DR GeneTree; ENSGT00390000002749; -.
DR HOGENOM; CLU_149699_0_0_1; -.
DR InParanoid; Q9DAQ9; -.
DR OMA; EHWLKKT; -.
DR PhylomeDB; Q9DAQ9; -.
DR TreeFam; TF337965; -.
DR BioGRID-ORCS; 75469; 1 hit in 58 CRISPR screens.
DR ChiTaRS; Spata19; mouse.
DR PRO; PR:Q9DAQ9; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9DAQ9; protein.
DR Bgee; ENSMUSG00000031991; Expressed in seminiferous tubule of testis and 27 other tissues.
DR ExpressionAtlas; Q9DAQ9; baseline and differential.
DR Genevisible; Q9DAQ9; MM.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0097225; C:sperm midpiece; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0030382; P:sperm mitochondrion organization; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR028219; SPATA19.
DR PANTHER; PTHR36468; PTHR36468; 1.
DR Pfam; PF15212; SPATA19; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Developmental protein;
KW Differentiation; Flagellum; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Phosphoprotein; Reference proteome;
KW Spermatogenesis; Transit peptide.
FT TRANSIT 1..24
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 25..154
FT /note="Spermatogenesis-associated protein 19,
FT mitochondrial"
FT /id="PRO_0000251610"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q920Q3"
FT MOD_RES 116
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q920Q3"
FT VAR_SEQ 1..35
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020764"
FT VAR_SEQ 36..45
FT /note="VSVVQHWLNK -> MSERMWGFGL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020765"
SQ SEQUENCE 154 AA; 18057 MW; 689884F870FC0B83 CRC64;
MIITTWIMYI FARKTVGLPF PPRVNSDIEV EESEAVSVVQ HWLNKTEEEA SRSIREKMSI
NDSPTHGHDI HVTRDLVKHH LSKSDMLTDP SQEVLEERTR IQFIRWSHTR IFQVPSEVMD
DVMQERIDQV RRSVSHLMCD SYNDPSFRTS CSEC
