SPT20_HUMAN
ID SPT20_HUMAN Reviewed; 786 AA.
AC Q8TB22; Q2TA99; Q2XUZ6; Q6P0P1; Q8WVW3; Q9H747;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Spermatogenesis-associated protein 20;
DE AltName: Full=Sperm-specific protein 411;
DE Short=Ssp411;
DE Flags: Precursor;
GN Name=SPATA20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
RC TISSUE=Testis;
RA Shi H.-J., Wu A.Z., Santos M., Feng Z.-M., Chen C.-L.C.;
RT "Cloning and characterization of human SSP411.";
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ARG-609.
RC TISSUE=Colon;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3), AND VARIANTS
RP GLU-88; THR-483 AND ARG-609.
RC TISSUE=Brain, Pancreas, Skin, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May play a role in fertility regulation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8TB22-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TB22-2; Sequence=VSP_023290;
CC Name=3;
CC IsoId=Q8TB22-3; Sequence=VSP_023288;
CC Name=4;
CC IsoId=Q8TB22-4; Sequence=VSP_023289, VSP_023291, VSP_023292;
CC -!- MISCELLANEOUS: [Isoform 3]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
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DR EMBL; DQ238597; ABB54669.1; -; mRNA.
DR EMBL; AK025000; BAB15051.1; -; mRNA.
DR EMBL; AC021491; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC017468; AAH17468.1; -; mRNA.
DR EMBL; BC025255; AAH25255.1; -; mRNA.
DR EMBL; BC111029; AAI11030.1; -; mRNA.
DR EMBL; BC065526; AAH65526.1; -; mRNA.
DR CCDS; CCDS11571.1; -. [Q8TB22-2]
DR CCDS; CCDS58563.1; -. [Q8TB22-1]
DR CCDS; CCDS58564.1; -. [Q8TB22-3]
DR RefSeq; NP_001245301.1; NM_001258372.1. [Q8TB22-1]
DR RefSeq; NP_001245302.1; NM_001258373.1. [Q8TB22-3]
DR RefSeq; NP_073738.2; NM_022827.3. [Q8TB22-2]
DR AlphaFoldDB; Q8TB22; -.
DR SMR; Q8TB22; -.
DR BioGRID; 122320; 56.
DR IntAct; Q8TB22; 65.
DR STRING; 9606.ENSP00000006658; -.
DR iPTMnet; Q8TB22; -.
DR PhosphoSitePlus; Q8TB22; -.
DR BioMuta; SPATA20; -.
DR DMDM; 311033529; -.
DR EPD; Q8TB22; -.
DR jPOST; Q8TB22; -.
DR MassIVE; Q8TB22; -.
DR MaxQB; Q8TB22; -.
DR PaxDb; Q8TB22; -.
DR PeptideAtlas; Q8TB22; -.
DR PRIDE; Q8TB22; -.
DR ProteomicsDB; 73947; -. [Q8TB22-1]
DR ProteomicsDB; 73948; -. [Q8TB22-2]
DR ProteomicsDB; 73949; -. [Q8TB22-3]
DR ProteomicsDB; 73950; -. [Q8TB22-4]
DR Antibodypedia; 18108; 61 antibodies from 17 providers.
DR DNASU; 64847; -.
DR Ensembl; ENST00000006658.11; ENSP00000006658.6; ENSG00000006282.21. [Q8TB22-2]
DR Ensembl; ENST00000356488.8; ENSP00000348878.4; ENSG00000006282.21. [Q8TB22-1]
DR Ensembl; ENST00000511937.5; ENSP00000489476.1; ENSG00000006282.21. [Q8TB22-4]
DR Ensembl; ENST00000619622.4; ENSP00000483295.1; ENSG00000006282.21. [Q8TB22-3]
DR GeneID; 64847; -.
DR KEGG; hsa:64847; -.
DR MANE-Select; ENST00000006658.11; ENSP00000006658.6; NM_022827.4; NP_073738.2. [Q8TB22-2]
DR UCSC; uc002ird.4; human. [Q8TB22-1]
DR CTD; 64847; -.
DR DisGeNET; 64847; -.
DR GeneCards; SPATA20; -.
DR HGNC; HGNC:26125; SPATA20.
DR HPA; ENSG00000006282; Tissue enhanced (testis).
DR MIM; 613939; gene.
DR neXtProt; NX_Q8TB22; -.
DR OpenTargets; ENSG00000006282; -.
DR PharmGKB; PA142670885; -.
DR VEuPathDB; HostDB:ENSG00000006282; -.
DR eggNOG; KOG2244; Eukaryota.
DR GeneTree; ENSGT00390000004836; -.
DR HOGENOM; CLU_014051_4_1_1; -.
DR InParanoid; Q8TB22; -.
DR OMA; PFYFGTY; -.
DR OrthoDB; 332120at2759; -.
DR PhylomeDB; Q8TB22; -.
DR TreeFam; TF105663; -.
DR PathwayCommons; Q8TB22; -.
DR SignaLink; Q8TB22; -.
DR BioGRID-ORCS; 64847; 11 hits in 1065 CRISPR screens.
DR ChiTaRS; SPATA20; human.
DR GenomeRNAi; 64847; -.
DR Pharos; Q8TB22; Tdark.
DR PRO; PR:Q8TB22; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q8TB22; protein.
DR Bgee; ENSG00000006282; Expressed in left testis and 197 other tissues.
DR ExpressionAtlas; Q8TB22; baseline and differential.
DR Genevisible; Q8TB22; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR CDD; cd02955; SSP411; 1.
DR Gene3D; 1.50.10.10; -; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR004879; DUF255.
DR InterPro; IPR024705; Ssp411.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR42899; PTHR42899; 1.
DR Pfam; PF03190; Thioredox_DsbH; 1.
DR PIRSF; PIRSF006402; UCP006402_thioredoxin; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Differentiation;
KW Phosphoprotein; Reference proteome; Secreted; Signal; Spermatogenesis.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..786
FT /note="Spermatogenesis-associated protein 20"
FT /id="PRO_0000278450"
FT REGION 23..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 649
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6T393"
FT VAR_SEQ 1..44
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023288"
FT VAR_SEQ 1..25
FT /note="MLGARAWLGRVLLLPRAGAGLAASR -> MSHLSSPPKKHKGEHKGHSLPHG
FT SE (in isoform 4)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_023289"
FT VAR_SEQ 26
FT /note="R -> RCPGVWPRTWPHRSPSR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023290"
FT VAR_SEQ 511..521
FT /note="LMVSGYAVTGA -> GAAHLRPSLSA (in isoform 4)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_023291"
FT VAR_SEQ 522..786
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_023292"
FT VARIANT 88
FT /note="Q -> E (in dbSNP:rs8076632)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_030777"
FT VARIANT 483
FT /note="S -> T (in dbSNP:rs9913430)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_030778"
FT VARIANT 609
FT /note="K -> R (in dbSNP:rs8065903)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_030779"
FT CONFLICT 356
FT /note="F -> L (in Ref. 2; BAB15051)"
FT /evidence="ECO:0000305"
FT CONFLICT 540
FT /note="K -> E (in Ref. 2; BAB15051)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 786 AA; 87899 MW; 00C3CCE7C7BD8438 CRC64;
MLGARAWLGR VLLLPRAGAG LAASRRGSSS RDKDRSATVS SSVPMPAGGK GSHPSSTPQR
VPNRLIHEKS PYLLQHAYNP VDWYPWGQEA FDKARKENKP IFLSVGYSTC HWCHMMEEES
FQNEEIGRLL SEDFVSVKVD REERPDVDKV YMTFVQATSS GGGWPMNVWL TPNLQPFVGG
TYFPPEDGLT RVGFRTVLLR IREQWKQNKN TLLENSQRVT TALLARSEIS VGDRQLPPSA
ATVNNRCFQQ LDEGYDEEYG GFAEAPKFPT PVILSFLFSY WLSHRLTQDG SRAQQMALHT
LKMMANGGIR DHVGQGFHRY STDRQWHVPH FEKMLYDQAQ LAVAYSQAFQ LSGDEFYSDV
AKGILQYVAR SLSHRSGGFY SAEDADSPPE RGQRPKEGAY YVWTVKEVQQ LLPEPVLGAT
EPLTSGQLLM KHYGLTEAGN ISPSQDPKGE LQGQNVLTVR YSLELTAARF GLDVEAVRTL
LNSGLEKLFQ ARKHRPKPHL DSKMLAAWNG LMVSGYAVTG AVLGQDRLIN YATNGAKFLK
RHMFDVASGR LMRTCYTGPG GTVEHSNPPC WGFLEDYAFV VRGLLDLYEA SQESAWLEWA
LRLQDTQDKL FWDSQGGGYF CSEAELGAGL PLRLKDDQDG AEPSANSVSA HNLLRLHGFT
GHKDWMDKCV CLLTAFSERM RRVPVALPEM VRALSAQQQT LKQIVICGDR QAKDTKALVQ
CVHSVYIPNK VLILADGDPS SFLSRQLPFL STLRRLEDQA TAYVCENQAC SVPITDPCEL
RKLLHP
