SPT20_MOUSE
ID SPT20_MOUSE Reviewed; 790 AA.
AC Q80YT5;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Spermatogenesis-associated protein 20;
DE AltName: Full=Sperm-specific protein 411;
DE Short=Ssp411;
DE AltName: Full=Transcript increased in spermiogenesis 78 protein;
GN Name=Spata20; Synonyms=Tisp78;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=15223837; DOI=10.1002/j.1939-4640.2004.tb02819.x;
RA Shi H.-J., Wu A.Z., Santos M., Feng Z.-M., Huang L., Chen Y.-M., Zhu K.,
RA Chen C.-L.C.;
RT "Cloning and characterization of rat spermatid protein SSP411: a
RT thioredoxin-like protein.";
RL J. Androl. 25:479-493(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in fertility regulation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Detected in 28- and 75-day-old mouse testes. No
CC expression detected at 21 days. {ECO:0000269|PubMed:15223837}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL645809; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL645965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC050788; AAH50788.1; -; mRNA.
DR CCDS; CCDS25256.1; -.
DR RefSeq; NP_659076.2; NM_144827.4.
DR AlphaFoldDB; Q80YT5; -.
DR SMR; Q80YT5; -.
DR BioGRID; 229844; 1.
DR IntAct; Q80YT5; 3.
DR MINT; Q80YT5; -.
DR STRING; 10090.ENSMUSP00000042572; -.
DR iPTMnet; Q80YT5; -.
DR PhosphoSitePlus; Q80YT5; -.
DR PaxDb; Q80YT5; -.
DR PeptideAtlas; Q80YT5; -.
DR PRIDE; Q80YT5; -.
DR ProteomicsDB; 257387; -.
DR Antibodypedia; 18108; 61 antibodies from 17 providers.
DR DNASU; 217116; -.
DR Ensembl; ENSMUST00000041705; ENSMUSP00000042572; ENSMUSG00000020867.
DR GeneID; 217116; -.
DR KEGG; mmu:217116; -.
DR UCSC; uc007kyt.1; mouse.
DR CTD; 64847; -.
DR MGI; MGI:2183449; Spata20.
DR VEuPathDB; HostDB:ENSMUSG00000020867; -.
DR eggNOG; KOG2244; Eukaryota.
DR GeneTree; ENSGT00390000004836; -.
DR HOGENOM; CLU_014051_4_1_1; -.
DR InParanoid; Q80YT5; -.
DR OMA; PFYFGTY; -.
DR OrthoDB; 332120at2759; -.
DR PhylomeDB; Q80YT5; -.
DR TreeFam; TF105663; -.
DR BioGRID-ORCS; 217116; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Spata20; mouse.
DR PRO; PR:Q80YT5; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q80YT5; protein.
DR Bgee; ENSMUSG00000020867; Expressed in spermatid and 22 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR CDD; cd02955; SSP411; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR004879; DUF255.
DR InterPro; IPR024705; Ssp411.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR42899; PTHR42899; 1.
DR Pfam; PF03190; Thioredox_DsbH; 1.
DR PIRSF; PIRSF006402; UCP006402_thioredoxin; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Phosphoprotein; Reference proteome;
KW Secreted; Spermatogenesis.
FT CHAIN 1..790
FT /note="Spermatogenesis-associated protein 20"
FT /id="PRO_0000278451"
FT REGION 1..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..33
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 5
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6T393"
FT MOD_RES 653
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6T393"
SQ SEQUENCE 790 AA; 88472 MW; 6B6D3B320FD1499E CRC64;
MSHHSSPPPK HKGEHKGHGL PRGSERGSSS RDKDRSATTV SSSAPMPAGG KSSRTNCPPP
APPKTVNRLI NEKSPYLLQH AYNPVDWYPW GQEAFDKAKK ENKPIFLSVG YSTCHWCHMM
EEESFQNEEI GRLLNENFIC VMVDREERPD VDKVYMTFVQ ATSSGGGWPM NVWLTPGLQP
FVGGTYFPPE DGLTRVGFRT VLMRICDQWK LNKNTLLENS QRVTTALLAR SEISVGDRQI
PASAATMNSR CFQQLDEGYD EEYGGFAEAP KFPTPVILNF LFSYWLSHRL TQDGSRAQQM
ALHTLKMMAN GGIQDHVGQG FHRYSTDRQW HIPHFEKMLY DQAQLSVVYT QAFQISGDEF
YADVAKGILQ YVTRTLSHRS GGFYSAEDAD SPPERGMKPQ EGAYYVWTVK EVQQLLPEPV
VGASEPLTSG QLLMKHYGLS EVGNINSSQD PNGELHGQNV LMVRYSLELT AARYGLEVEA
VRALLNTGLE KLFQARKHRP KAHLDNKMLA AWNGLMVSGF AVTGAALGME KLVAQATSGA
KFLKRHMFDV SSGRLKRTCY AGTGGTVEQS NPPCWGFLED YAFVVRGLLD LYEASQESSW
LEWALRLQDT QDKLFWDPRG GGYFCSEAEL GADLPLRLKD DQDGAEPSAN SVSAHNLLRL
HSFTGHKDWM DKCVCLLTAF SERMRRVPVA LPEMVRTLSA QQQTLKQIVI CGDPQAKDTK
ALLQCVHSIY VPNKVLILAD GDPSSFLSRQ LPFLSSLRRV EDRATVYIFE NQACSMPITD
PCELRKLLHQ
