ID   SPT20_RAT               Reviewed;         789 AA.
AC   Q6T393;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Spermatogenesis-associated protein 20;
DE   AltName: Full=Sperm-specific protein 411;
DE            Short=Ssp411;
GN   Name=Spata20;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Testis;
RX   PubMed=15223837; DOI=10.1002/j.1939-4640.2004.tb02819.x;
RA   Shi H.-J., Wu A.Z., Santos M., Feng Z.-M., Huang L., Chen Y.-M., Zhu K.,
RA   Chen C.-L.C.;
RT   "Cloning and characterization of rat spermatid protein SSP411: a
RT   thioredoxin-like protein.";
RL   J. Androl. 25:479-493(2004).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5 AND SER-652, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: May play a role in fertility regulation.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Testis-specific and age-dependent (at protein
CC       level). Highly expressed. Expressed in round spermatids located in the
CC       inner half-layer of the seminiferous epithelium as well as in early
CC       elongated spermatids having cytoplasmic protrusions into the tubular
CC       lumen.
CC   -!- DEVELOPMENTAL STAGE: Detected at age 28 days and was most abundant at
CC       age 63 days. No expression at 21 days, seems therefore to be expressed
CC       in spermatids and not in spermatocytes or spermatogonia of the germ
CC       cells.
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DR   EMBL; AY438568; AAR12892.1; -; mRNA.
DR   RefSeq; NP_955434.1; NM_199402.1.
DR   AlphaFoldDB; Q6T393; -.
DR   STRING; 10116.ENSRNOP00000004377; -.
DR   iPTMnet; Q6T393; -.
DR   PhosphoSitePlus; Q6T393; -.
DR   PaxDb; Q6T393; -.
DR   GeneID; 360604; -.
DR   KEGG; rno:360604; -.
DR   CTD; 64847; -.
DR   RGD; 735087; Spata20.
DR   eggNOG; KOG2244; Eukaryota.
DR   InParanoid; Q6T393; -.
DR   OrthoDB; 332120at2759; -.
DR   PhylomeDB; Q6T393; -.
DR   PRO; PR:Q6T393; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007283; P:spermatogenesis; NAS:RGD.
DR   CDD; cd02955; SSP411; 1.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR004879; DUF255.
DR   InterPro; IPR024705; Ssp411.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR42899; PTHR42899; 1.
DR   Pfam; PF03190; Thioredox_DsbH; 1.
DR   PIRSF; PIRSF006402; UCP006402_thioredoxin; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Differentiation; Phosphoprotein; Reference proteome;
KW   Secreted; Spermatogenesis.
FT   CHAIN           1..789
FT                   /note="Spermatogenesis-associated protein 20"
FT                   /id="PRO_0000278452"
FT   REGION          1..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        12..32
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        33..48
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         5
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         652
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
SQ   SEQUENCE   789 AA;  88191 MW;  0D7C1F7E56931C84 CRC64;
     MSHHSPPPPK HKGEHKGHGL PRGSERGSSS RGKDRSASVS NSVPMPAGGK ASRTNCPPPA
     PQKTANRLIN EKSPYLLQHA HNPVDWYPWG QEAFDKAKKE NKPIFLSVGY STCHWCHMME
     EESFQNEEIG HLLNENFVSV MVDREERPDV DKVYMTFVQA TSSGGGWPMN VWLTPSLQPF
     VGGTYFPPED GLTRVGFRTV LMRICDQWKQ NKNTLLENSQ RVTTALLARS EISVGDRQLP
     PSAATMNSRC FQQLDEGYDE EYGGFAEAPK FPTPVILNFL FSYWLSHRVT QDGSRAQQMA
     LHTLKMMANG GIRDHVGQGF HRYSTDRQWH IPHFEKMLYD QAQLSVVYCQ AFQISGDEFF
     SDVAKGILQY VTRNLSHRSG GFYSAEDADS PPERGVKPQE GALYLWTVKE VQQLLPEPVG
     GASEPLTSGQ LLMKHYGLSE AGNINPTQDV NGEMHGQNVL TVRDSLELTG ARYGLEVEAV
     RALLNTGLEK LFQARKHRPK AHLDNKMLAA WNGLMVSGFA VAGSVLGMEK LVTQATNGAK
     FLKRHMFDVS SGRLKRTCYA GAGGTVEQSN PPCWGFLEDY AFVVRGLLDL YEASQESSWL
     EWALRLQDIQ DKLFWDSHGG GYFCSEAELG TDLPLRLKDD QDGAEPSANS VSAHNLLRLH
     GLTGHKDWMD KCVCLLTAFS ERMRRVPVAL PEMVRALSAQ QQTLKQIVIC GDPQAKDTKA
     LLQCVHSIYI PNKVLILADG DPSSFLSRQL PFLSNLRRVE DRATVYIFEN QACSMPITDP
     CELRKLLHQ