SPT21_MOUSE
ID SPT21_MOUSE Reviewed; 681 AA.
AC Q8BHW6;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Spermatogenesis-associated protein 21;
GN Name=Spata21;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Involved in the differentiation of haploid spermatids.
CC {ECO:0000250}.
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DR EMBL; AK077155; BAC36647.1; -; mRNA.
DR EMBL; AL645625; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC112427; AAI12428.1; -; mRNA.
DR EMBL; BC113132; AAI13133.1; -; mRNA.
DR CCDS; CCDS18863.1; -.
DR RefSeq; NP_808535.1; NM_177867.3.
DR AlphaFoldDB; Q8BHW6; -.
DR SMR; Q8BHW6; -.
DR STRING; 10090.ENSMUSP00000053080; -.
DR iPTMnet; Q8BHW6; -.
DR PhosphoSitePlus; Q8BHW6; -.
DR PaxDb; Q8BHW6; -.
DR PeptideAtlas; Q8BHW6; -.
DR PRIDE; Q8BHW6; -.
DR ProteomicsDB; 263333; -.
DR Antibodypedia; 47956; 68 antibodies from 15 providers.
DR DNASU; 329972; -.
DR Ensembl; ENSMUST00000051907; ENSMUSP00000053080; ENSMUSG00000045004.
DR GeneID; 329972; -.
DR KEGG; mmu:329972; -.
DR UCSC; uc008vnv.1; mouse.
DR CTD; 374955; -.
DR MGI; MGI:3607787; Spata21.
DR VEuPathDB; HostDB:ENSMUSG00000045004; -.
DR eggNOG; KOG0027; Eukaryota.
DR GeneTree; ENSGT00940000162494; -.
DR HOGENOM; CLU_028005_0_0_1; -.
DR InParanoid; Q8BHW6; -.
DR OMA; PCIPLYP; -.
DR OrthoDB; 357989at2759; -.
DR PhylomeDB; Q8BHW6; -.
DR TreeFam; TF328393; -.
DR BioGRID-ORCS; 329972; 4 hits in 71 CRISPR screens.
DR ChiTaRS; Spata21; mouse.
DR PRO; PR:Q8BHW6; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BHW6; protein.
DR Bgee; ENSMUSG00000045004; Expressed in spermatid and 21 other tissues.
DR Genevisible; Q8BHW6; MM.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR043520; SPT21/EFCAB3.
DR PANTHER; PTHR47500; PTHR47500; 1.
DR SMART; SM00054; EFh; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Reference proteome.
FT CHAIN 1..681
FT /note="Spermatogenesis-associated protein 21"
FT /id="PRO_0000330688"
FT DOMAIN 464..499
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 653..681
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 70..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..177
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..280
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 312..336
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 477
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 479
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 481
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 483
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 488
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 681 AA; 75541 MW; 127C7FDD0F4F3DAD CRC64;
MENRNTHTHP ESKADAPAVQ SSSGLSSTKK KTDSEPTVSA GQTGLADATE RDNRKQPVAP
RGPERELRPT GSSQDGSGEL PTQEPSARPR TTQDGPRELQ AGPEQVQPSG DFVASEGRPQ
PAMQTKDQQK RWQSAEVKEI LLAESCQGNT DHDLGKPRPS NSRSQPLKNN SPSEAGRPQV
RLQEPMPAPG SGTHKDNPQE AVPPKPKVTA EEKKAPPVLP SVPGPRTHKD RGEAAEIRAT
LRLQPPPPPL PEERDTEKKE LGQGQKQRQQ ALSAAGTQGP ANSRRGFMKC LLEVEEQEEA
THRRTLKSRG LTARRSPKTV TSVSTSGPIS SSVPTLPLTL HEPSTSAPAS VPSWVRPPAP
GQTPIPMGSP GSVLPTSAQD QNWRWPEFLP QGNERTLTYA KILRQEPEEH SLFRMYQSWE
ERTEEHLTLK QEEAFRSYFD IFNGPGEVDA RSLKNILLLI GFTFTPAQVE EALMSADVNG
DGHVDFKDFL AVMTDTKRFF CSVEQNVLMD MSPRNPYTLF FEILSLLVEM LALPELALEE
ITNYYQKKLK EGSSKAREME SAMGGLRQRK KIPYNPQQVE NLEVPERRVL RILSRLKQQN
YAANLQSPYA QVPCIPLCPR LDKKAVRRKL ASHNHSVLDQ CVSTSLGPDF HGLFFQPGQQ
GSREHSSDSR KWLSSMPART H
