SPT21_RAT
ID SPT21_RAT Reviewed; 685 AA.
AC Q68A65;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Spermatogenesis-associated protein 21;
DE AltName: Full=Spermatogenic cell-specific gene 3 protein;
DE Short=Spergen-3;
GN Name=Spata21; Synonyms=Spergen3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND FUNCTION.
RC STRAIN=Sprague-Dawley; TISSUE=Testis;
RX PubMed=15477360; DOI=10.1002/j.1939-4640.2004.tb03158.x;
RA Iida H., Yamashita H., Doiguchi M., Kaneko T.;
RT "Molecular cloning of rat Spergen-3, a spermatogenic cell-specific gene-3,
RT encoding a novel 75-kDa protein bearing EF-hand motifs.";
RL J. Androl. 25:885-892(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Involved in the differentiation of haploid spermatids.
CC {ECO:0000269|PubMed:15477360}.
CC -!- TISSUE SPECIFICITY: Expressed in testis. Exclusively expressed in
CC haploid spermatids and very weakly in spermatogonia, spermatocytes, and
CC Sertoli cells as well as in interstitial cell.
CC {ECO:0000269|PubMed:15477360}.
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DR EMBL; AB168097; BAD42380.1; -; mRNA.
DR EMBL; BC089089; AAH89089.1; -; mRNA.
DR RefSeq; NP_001004447.1; NM_001004447.1.
DR RefSeq; XP_008762499.1; XM_008764277.2.
DR RefSeq; XP_017449036.1; XM_017593547.1.
DR AlphaFoldDB; Q68A65; -.
DR STRING; 10116.ENSRNOP00000012147; -.
DR iPTMnet; Q68A65; -.
DR PhosphoSitePlus; Q68A65; -.
DR PaxDb; Q68A65; -.
DR Ensembl; ENSRNOT00000012147; ENSRNOP00000012147; ENSRNOG00000009058.
DR GeneID; 366491; -.
DR KEGG; rno:366491; -.
DR CTD; 374955; -.
DR RGD; 1302954; Spata21.
DR eggNOG; KOG0027; Eukaryota.
DR GeneTree; ENSGT00940000162494; -.
DR HOGENOM; CLU_028005_0_0_1; -.
DR InParanoid; Q68A65; -.
DR OMA; PCIPLYP; -.
DR OrthoDB; 357989at2759; -.
DR PhylomeDB; Q68A65; -.
DR TreeFam; TF328393; -.
DR PRO; PR:Q68A65; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000009058; Expressed in testis and 2 other tissues.
DR Genevisible; Q68A65; RN.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR043520; SPT21/EFCAB3.
DR PANTHER; PTHR47500; PTHR47500; 1.
DR SMART; SM00054; EFh; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Reference proteome.
FT CHAIN 1..685
FT /note="Spermatogenesis-associated protein 21"
FT /id="PRO_0000330689"
FT DOMAIN 468..503
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 302..385
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 656..685
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..73
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..179
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 239..255
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..334
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 481
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 483
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 485
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 487
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 492
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 685 AA; 75826 MW; 6406A77B30605668 CRC64;
MENRNTHTHP ENKAEAPTVQ SSSGLSSTKK KTDLEAAVAA GQNGRADATE RDSRKKPAAP
RGPEKEPRHT ASSQERSGEP QTQEQRPSAR SKTTQDGPRE LQTGQEQARP SGDFVASEGR
PQPAMQTKDQ QKRWQSAEAK DVQSTESCQG NTDHDLGKPC TSDSRSHPQK TNSLSEGGRP
QARLQEPMPA PSSGTHKDNP QEAVPPKPKV TAEEKKAPPV LPTVPRTHKD RGEAVESKAS
PRPPPPPPSP PPPPPPEERD VEKKELGQGQ KQRQPALAAA GTQGPGNFRR GFMKCLLEVE
EQEEATHRRT LKSRALTARR SPKTVTSVPT SGPVNSSVPP LPPTLHEPST SAPATVPSWV
KPPAPGQAPI PMGTPSSVLP TPAQDQSWRW PELLPQGNER NLNYAKTRQE SEEHGLFKLY
QNWEERTEEH LTLKQEEAFR SYFDIFNGPG EVDARSLKNI LLLIGFTFTP AQVEEALMSA
DVNGDGHVDF KDFLAVMTDT KRFFCSVEQN VLMDMSPPNP YTLFFEILSL LVEMLALPEV
ALEEITNYYQ KKLKEGSSKA REMETAMGGI RQRKKIPYSS QQVENLEVPE RRVLRILSRL
KQQNYAANLQ SPYAQVPCIP LCPRLDKKAV RRKQASHNHS VLDQCVPTSL GPDLHGFFLQ
PGQQGSREHS SDSRKWLSSV PARTH
