SPT22_MOUSE
ID SPT22_MOUSE Reviewed; 358 AA.
AC Q5SV06;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Spermatogenesis-associated protein 22 {ECO:0000305};
GN Name=Spata22 {ECO:0000312|MGI:MGI:2685728}; Synonyms=Gm882, Repro42;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-187.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=22011390; DOI=10.1095/biolreprod.111.095752;
RA La Salle S., Palmer K., O'Brien M., Schimenti J.C., Eppig J., Handel M.A.;
RT "Spata22, a novel vertebrate-specific gene, is required for meiotic
RT progress in mouse germ cells.";
RL Biol. Reprod. 86:45-45(2012).
RN [4]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH MEIOB AND RPA2.
RX PubMed=24240703; DOI=10.1038/ncomms3788;
RA Luo M., Yang F., Leu N.A., Landaiche J., Handel M.A., Benavente R.,
RA La Salle S., Wang P.J.;
RT "MEIOB exhibits single-stranded DNA-binding and exonuclease activities and
RT is essential for meiotic recombination.";
RL Nat. Commun. 4:2788-2788(2013).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=30272023; DOI=10.1038/s42003-018-0154-z;
RA Zhang Q., Shao J., Fan H.Y., Yu C.;
RT "Evolutionarily-conserved MZIP2 is essential for crossover formation in
RT mammalian meiosis.";
RL Commun. Biol. 1:147-147(2018).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=30746471; DOI=10.1126/sciadv.aau9780;
RA Zhang Q., Ji S.Y., Busayavalasa K., Yu C.;
RT "SPO16 binds SHOC1 to promote homologous recombination and crossing-over in
RT meiotic prophase I.";
RL Sci. Adv. 5:eaau9780-eaau9780(2019).
RN [7]
RP INTERACTION WITH BRME1.
RX PubMed=32345962; DOI=10.1038/s41467-020-15954-x;
RA Zhang J., Gurusaran M., Fujiwara Y., Zhang K., Echbarthi M., Vorontsov E.,
RA Guo R., Pendlebury D.F., Alam I., Livera G., Emmanuelle M., Wang P.J.,
RA Nandakumar J., Davies O.R., Shibuya H.;
RT "The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs
RT the mitotic BRCA2-RAD51 function in cancer cells.";
RL Nat. Commun. 11:2055-2055(2020).
CC -!- FUNCTION: Meiosis-specific protein required for homologous
CC recombination in meiosis I. {ECO:0000269|PubMed:22011390}.
CC -!- SUBUNIT: Component of a multiprotein complex with MEIOB and RPA2
CC (PubMed:24240703). Interacts with the complex BRME1:HSF2BP:BRCA2
CC (PubMed:32345962). {ECO:0000269|PubMed:24240703,
CC ECO:0000269|PubMed:32345962}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:24240703,
CC ECO:0000269|PubMed:30272023, ECO:0000269|PubMed:30746471}.
CC Note=Localizes on meiotic chromosome axes. Accumulates on resected DNA.
CC Localization is dependent on MEIOB. {ECO:0000269|PubMed:24240703}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in gonadal germ cells, when
CC male and female germ cells progress through prophase of meiosis I.
CC {ECO:0000269|PubMed:22011390}.
CC -!- DEVELOPMENTAL STAGE: In testis, weakly present from 8 dpp, and readily
CC detectable from 10 dpp, with expression sustained through adulthood.
CC Present in all populations of spermatocytes (at protein level).
CC {ECO:0000269|PubMed:22011390}.
CC -!- DISRUPTION PHENOTYPE: Mice develop and grow normally but show
CC infertility in both sexes. Gametogenesis is affected with germ cells
CC that do not progress beyond early meiotic prophase, with subsequent
CC germ cell loss in both males and females.
CC {ECO:0000269|PubMed:22011390}.
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DR EMBL; AL645739; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BB616785; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS36219.1; -.
DR RefSeq; NP_001038996.1; NM_001045531.1.
DR RefSeq; XP_011247397.1; XM_011249095.1.
DR RefSeq; XP_011247398.1; XM_011249096.2.
DR RefSeq; XP_011247399.1; XM_011249097.1.
DR AlphaFoldDB; Q5SV06; -.
DR CORUM; Q5SV06; -.
DR STRING; 10090.ENSMUSP00000090602; -.
DR iPTMnet; Q5SV06; -.
DR PhosphoSitePlus; Q5SV06; -.
DR PaxDb; Q5SV06; -.
DR PRIDE; Q5SV06; -.
DR ProteomicsDB; 254544; -.
DR Ensembl; ENSMUST00000092926; ENSMUSP00000090602; ENSMUSG00000112920.
DR GeneID; 380709; -.
DR KEGG; mmu:380709; -.
DR UCSC; uc007kan.1; mouse.
DR CTD; 84690; -.
DR MGI; MGI:2685728; Spata22.
DR VEuPathDB; HostDB:ENSMUSG00000112920; -.
DR eggNOG; ENOG502RNNP; Eukaryota.
DR GeneTree; ENSGT00390000018151; -.
DR HOGENOM; CLU_050539_0_1_1; -.
DR InParanoid; Q5SV06; -.
DR OMA; NIFRCVS; -.
DR OrthoDB; 938712at2759; -.
DR PhylomeDB; Q5SV06; -.
DR TreeFam; TF332549; -.
DR BioGRID-ORCS; 380709; 0 hits in 106 CRISPR screens.
DR PRO; PR:Q5SV06; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5SV06; protein.
DR Bgee; ENSMUSG00000112920; Expressed in spermatocyte and 22 other tissues.
DR ExpressionAtlas; Q5SV06; baseline and differential.
DR GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR GO; GO:0009566; P:fertilization; IMP:MGI.
DR GO; GO:0007276; P:gamete generation; IMP:MGI.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:MGI.
DR GO; GO:0000711; P:meiotic DNA repair synthesis; IMP:MGI.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IMP:MGI.
DR GO; GO:0061458; P:reproductive system development; IMP:MGI.
DR GO; GO:0048137; P:spermatocyte division; ISO:MGI.
DR InterPro; IPR033536; Spata22.
DR PANTHER; PTHR35258; PTHR35258; 1.
PE 1: Evidence at protein level;
KW Chromosome; Meiosis; Reference proteome.
FT CHAIN 1..358
FT /note="Spermatogenesis-associated protein 22"
FT /id="PRO_0000251607"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 81..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 358 AA; 40163 MW; B1470E3E1642E0D8 CRC64;
MKRNLNESSA RSTAGCLPVP LFNQKKRNRQ PLTSNPLQND PGVSTVSDSY GSPSFPTDWA
WEAVNPEVAP LKKTVNTGQI PASASYPWRS QDSVSKSIQS NAERSQSAWG YRGNNRNTSL
RTWDFRPQHK TVSPAANSEF SSCPVNLGAQ QQKQFQTPEF PNLPGHKEAE VPRQTCLSKL
PGSTMKGPDR ASALQAFKPS FQQNPFKKTV LGDIPRENSL KEGTLHQLKE KDNSLRIISA
VIESMKYWRA HVQKTVLLFE ILAVLDSAVT SGPHYSKTFL MRDGKNILPC VFYEIDRELP
RLIRGRVHRC VGHYDPDKNI FKCVSVRPAS ASEQKTFQAF VTIADAEMKY HTKVTNEM
