SPT2_DROME
ID SPT2_DROME Reviewed; 581 AA.
AC Q8IMP6; Q8SYF3; Q9Y132;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Protein SPT2 homolog;
GN ORFNames=CG5815;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IMP6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IMP6-2; Sequence=VSP_030692, VSP_030693;
CC -!- SIMILARITY: Belongs to the SPT2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL49211.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAN14396.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56668.1; -; Genomic_DNA.
DR EMBL; AY071589; AAL49211.1; ALT_FRAME; mRNA.
DR RefSeq; NP_651537.3; NM_143280.3.
DR RefSeq; NP_733196.1; NM_170317.2. [Q8IMP6-1]
DR AlphaFoldDB; Q8IMP6; -.
DR BioGRID; 68155; 2.
DR IntAct; Q8IMP6; 8.
DR STRING; 7227.FBpp0084476; -.
DR PaxDb; Q8IMP6; -.
DR PRIDE; Q8IMP6; -.
DR EnsemblMetazoa; FBtr0085106; FBpp0084476; FBgn0027574. [Q8IMP6-1]
DR GeneID; 43266; -.
DR KEGG; dme:Dmel_CG5815; -.
DR UCSC; CG5815-RA; d. melanogaster. [Q8IMP6-1]
DR FlyBase; FBgn0027574; CG5815.
DR VEuPathDB; VectorBase:FBgn0027574; -.
DR eggNOG; ENOG502QWHS; Eukaryota.
DR InParanoid; Q8IMP6; -.
DR PhylomeDB; Q8IMP6; -.
DR BioGRID-ORCS; 43266; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43266; -.
DR PRO; PR:Q8IMP6; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0027574; Expressed in cleaving embryo and 24 other tissues.
DR ExpressionAtlas; Q8IMP6; baseline and differential.
DR Genevisible; Q8IMP6; DM.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0043486; P:histone exchange; IBA:GO_Central.
DR GO; GO:0006334; P:nucleosome assembly; IBA:GO_Central.
DR GO; GO:0010847; P:regulation of chromatin assembly; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR InterPro; IPR013256; Chromatin_SPT2.
DR Pfam; PF08243; SPT2; 1.
DR SMART; SM00784; SPT2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Reference proteome.
FT CHAIN 1..581
FT /note="Protein SPT2 homolog"
FT /id="PRO_0000315741"
FT REGION 26..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..495
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 36..76
FT /evidence="ECO:0000255"
FT COILED 276..307
FT /evidence="ECO:0000255"
FT COMPBIAS 30..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 160..183
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 184..216
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..242
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..314
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 333..416
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 446..460
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 466..488
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 554
FT /note="M -> S (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_030692"
FT VAR_SEQ 555..581
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_030693"
SQ SEQUENCE 581 AA; 65701 MW; ED7D0FC1A4BC29B0 CRC64;
MDFGDLLRYA KKNSDAVTKE VGQGKYYSTK YSPPKKQSKE SKQLSSNIQK FLQKKEAEEA
EKKRLERQKL NDLLAKRDEK SKNKIRKMLK VTKSANKSVL EDAKDYEGAI DGHEAGEGQG
DDYGYVSTEA NAFYDKYIEK VRDVQEDKGF TPSRPQSLKD LSGTKERVKA AITREREEAK
GNTRQKSSTS TLPSSATKSK ESSVARSYST SKTLYDPNAE KLEEERKKRQ EEEQRRAKIK
RPAQPPPMDF QALLRLAEKK QHEPVVFEVE KKKEPERLLS AREKRELEER QRQQEQRAQR
LKMRESEGKE TPKSIPNRME PNGRIPKLNQ AKPANAPSDS FKKPTAPQPT KSSASSTSLS
SSNSHSSASR SSVSSSRPAT KSAQLTARPG ATASAVGKPS SSSSRDVPSK NPYAATSLKG
TVREFPPRDQ SSISTLDRRK IPAAAKTRQT PSSDVQRSQG GRQFPPADVK RRKPNEPPVT
KRRIYDDDDE DEYDSELDDF IDDGDCEEDI SSHIRDIFGY DKRRYQGIDD DDRGMESSFA
QVQREEFISK KLGMQEDLED MRMEAAHKKQ KKLVAKISSR S
