SPT31_MOUSE
ID SPT31_MOUSE Reviewed; 1014 AA.
AC E9QAF0; Q5MAT5;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Spermatogenesis-associated protein 31;
DE AltName: Full=Acrosome-expressed protein 1;
GN Name=Spata31; Synonyms=Aep1, Vad1.3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=16924657; DOI=10.1002/jcp.20746;
RA Luk J.M., Lee N.P., Shum C.K., Lam B.Y., Siu A.F., Che C.M., Tam P.C.,
RA Cheung A.N., Yang Z.M., Lin Y.N., Matzuk M.M., Lee K.F., Yeung W.S.;
RT "Acrosome-specific gene AEP1: identification, characterization and roles in
RT spermatogenesis.";
RL J. Cell. Physiol. 209:755-766(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH ACTB AND SYNTAXIN 1.
RX PubMed=20850414; DOI=10.1016/j.bbrc.2010.09.049;
RA Zuo Y., Gao J., Yeung W.S., Lee K.F.;
RT "The testis-specific VAD1.3/AEP1 interacts with ?-actin and syntaxin 1 and
RT directs peri-nuclear/Golgi expression with bipartite nucleus localization
RT (BNL) sequence.";
RL Biochem. Biophys. Res. Commun. 401:275-280(2010).
CC -!- FUNCTION: May play a role in spermatogenesis. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ACTB and STX1A and/or STX1B.
CC {ECO:0000269|PubMed:20850414}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome lumen
CC {ECO:0000269|PubMed:20850414}. Note=Localizes to the inner and outer
CC membranes as well as to the matrix of the acrosome.
CC -!- SIMILARITY: Belongs to the SPATA31 family. {ECO:0000305}.
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DR EMBL; AY842249; AAW29519.1; -; mRNA.
DR EMBL; AC159190; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS26602.1; -.
DR RefSeq; NP_084323.2; NM_030047.2.
DR AlphaFoldDB; E9QAF0; -.
DR STRING; 10090.ENSMUSP00000097025; -.
DR iPTMnet; E9QAF0; -.
DR PhosphoSitePlus; E9QAF0; -.
DR PaxDb; E9QAF0; -.
DR PRIDE; E9QAF0; -.
DR ProteomicsDB; 254545; -.
DR Ensembl; ENSMUST00000070216; ENSMUSP00000097025; ENSMUSG00000056223.
DR GeneID; 78124; -.
DR KEGG; mmu:78124; -.
DR UCSC; uc007qza.2; mouse.
DR CTD; 78124; -.
DR MGI; MGI:1925374; Spata31.
DR VEuPathDB; HostDB:ENSMUSG00000056223; -.
DR eggNOG; ENOG502RU0E; Eukaryota.
DR GeneTree; ENSGT00950000183043; -.
DR HOGENOM; CLU_005668_2_1_1; -.
DR InParanoid; E9QAF0; -.
DR OMA; CHLVSPH; -.
DR OrthoDB; 1095565at2759; -.
DR PhylomeDB; E9QAF0; -.
DR TreeFam; TF338531; -.
DR BioGRID-ORCS; 78124; 1 hit in 71 CRISPR screens.
DR PRO; PR:E9QAF0; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; E9QAF0; protein.
DR Bgee; ENSMUSG00000056223; Expressed in testis and 5 other tissues.
DR ExpressionAtlas; E9QAF0; baseline and differential.
DR GO; GO:0043160; C:acrosomal lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0002080; C:acrosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0001669; C:acrosomal vesicle; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003779; F:actin binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR039509; SPATA31/FAM205.
DR Pfam; PF14650; FAM75; 2.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Differentiation; Membrane; Reference proteome;
KW Spermatogenesis; Transmembrane; Transmembrane helix.
FT CHAIN 1..1014
FT /note="Spermatogenesis-associated protein 31"
FT /id="PRO_0000420572"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 925..961
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 55
FT /note="N -> D (in Ref. 1; AAW29519)"
FT /evidence="ECO:0000305"
FT CONFLICT 87
FT /note="W -> R (in Ref. 1; AAW29519)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="S -> P (in Ref. 1; AAW29519)"
FT /evidence="ECO:0000305"
FT CONFLICT 385
FT /note="L -> S (in Ref. 1; AAW29519)"
FT /evidence="ECO:0000305"
FT CONFLICT 581
FT /note="Q -> R (in Ref. 1; AAW29519)"
FT /evidence="ECO:0000305"
FT CONFLICT 875
FT /note="A -> V (in Ref. 1; AAW29519)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1014 AA; 115834 MW; 1B8895A97F411B84 CRC64;
MESLPSLLES IYTTWLSLSS TIWAMDMILA FVCGLGLYHL LLPFLESHLS SPPSNIKFTR
KPQIQMTWQS QFKKKFRNHC RNDAKAWGEC LKKLKEKEKD KLFLEEMSPG HHLNSLGNIF
NSSSAKQDST TLSPFWNLKE KSEEQVATQK LSYPKISEDH FQQKCDQLFW GLPSLHSESL
VAAAWIPQTT STLPSPFFLF NVISSVYPIQ LQDKMSPMLP HTHPLSYLDL QSPHLILSPL
EFQTPALNLP VLLPSSLPYN SDFGTSYSQS QSKPQYLPTE IKYNKKPSLA KQIENRSTLP
LMVQKPQEAY DILAPNPSQD WVVSILPDNF PICRELREKL EQHIQKWLIQ HQWNFPHKIQ
VSEKMKELQN TVIGNCQTRD KPGTLQALGE HSNEGQKRKF QLEKESGKNL GPILGKISKD
PIRGLEKTTA IKDLENNLKA HLGTKSGQID QGLTPLSMRQ SWLAVDDSFY EMENNLTSLK
SSAKSMCSSE KLAFLKPETR QALEAHIVRF WAKHRWSLPL KILKPVKLFQ LSIESLPVVL
CAQTSSTTSV HRTRSAAEVV RFLGKPCLRQ MIIEDSSPSP QNLLLVSSPS CKRARRRLPF
GVDHEPSTAL PTKPECGHIS EDLTYNFMNT TSQTRTIFKK EIETREVVLL PRRTSDQNLE
AYKHQEKVVS EFPHNVETEL AGQPQIYTTT VLPPKRSRSM PLPVDTLTSH VLGDIVVADM
DNSLVQQRPS TPKHLVSQKS QIKMLAPTYQ SEGTKRQSEI KYEDRPKLTP VTEKKANFGS
QYYQTLPKIA QVLPGRPPQR HLGRFLQWIH PKKTIKGHEF HPLKGNPTAA IVQNQRRQVR
KIPYMDNNVT EAQELMTTVG QMLEKKMMLQ HQPYASKFNQ HREVPPAPMS RFSHGHMPVS
YLQQRRAPSY PGSCSCQRCS VQNRHIRNQL PQRSVRFSKK PQNPRNPSHK KPQNPRNPSR
LSRNAALNLV NSQNRTIVPG NSNHHLYCPR HCALQRDVCR ELGHSSVVFP NRKT
