SPT32_HUMAN
ID SPT32_HUMAN Reviewed; 384 AA.
AC Q96LK8; Q7Z4U1; Q8N6V6;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 3.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Spermatogenesis-associated protein 32;
DE AltName: Full=Testis-expressed protein 34;
GN Name=SPATA32; Synonyms=C17orf46, TEX34;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT MET-142.
RA Guo J.H., Yu L.;
RL Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT MET-142.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH SYNTAXIN-1 AND ACTB.
RX PubMed=18621766; DOI=10.1093/molehr/gan041;
RA Lee K.F., Tam Y.T., Zuo Y., Cheong A.W., Pang R.T., Lee N.P., Shum C.K.,
RA Tam P.C., Cheung A.N., Yang Z.M., Yeung W.S., Luk J.M.;
RT "Characterization of an acrosome protein VAD1.2/AEP2 which is
RT differentially expressed in spermatogenesis.";
RL Mol. Hum. Reprod. 14:465-474(2008).
CC -!- SUBUNIT: Interacts with syntaxin-1 and ACTB.
CC {ECO:0000269|PubMed:18621766}.
CC -!- INTERACTION:
CC Q96LK8; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-12849978, EBI-741158;
CC Q96LK8; Q92569: PIK3R3; NbExp=3; IntAct=EBI-12849978, EBI-79893;
CC -!- TISSUE SPECIFICITY: Detected in testis, and on the acrosomal cap of
CC spermatids.
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DR EMBL; AK058143; BAB71683.1; -; mRNA.
DR EMBL; AF433663; AAP97314.1; -; mRNA.
DR EMBL; AC008105; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC028124; AAH28124.1; -; mRNA.
DR CCDS; CCDS32669.1; -.
DR RefSeq; NP_689556.2; NM_152343.2.
DR AlphaFoldDB; Q96LK8; -.
DR BioGRID; 125887; 4.
DR IntAct; Q96LK8; 2.
DR STRING; 9606.ENSP00000331532; -.
DR iPTMnet; Q96LK8; -.
DR PhosphoSitePlus; Q96LK8; -.
DR BioMuta; SPATA32; -.
DR DMDM; 296439385; -.
DR MassIVE; Q96LK8; -.
DR PaxDb; Q96LK8; -.
DR PeptideAtlas; Q96LK8; -.
DR PRIDE; Q96LK8; -.
DR ProteomicsDB; 77218; -.
DR Antibodypedia; 17631; 14 antibodies from 8 providers.
DR DNASU; 124783; -.
DR Ensembl; ENST00000331780.5; ENSP00000331532.4; ENSG00000184361.13.
DR GeneID; 124783; -.
DR KEGG; hsa:124783; -.
DR MANE-Select; ENST00000331780.5; ENSP00000331532.4; NM_152343.3; NP_689556.2.
DR UCSC; uc002iis.1; human.
DR CTD; 124783; -.
DR DisGeNET; 124783; -.
DR GeneCards; SPATA32; -.
DR HGNC; HGNC:26349; SPATA32.
DR HPA; ENSG00000184361; Tissue enriched (testis).
DR neXtProt; NX_Q96LK8; -.
DR OpenTargets; ENSG00000184361; -.
DR PharmGKB; PA142672229; -.
DR VEuPathDB; HostDB:ENSG00000184361; -.
DR eggNOG; ENOG502RU2T; Eukaryota.
DR GeneTree; ENSGT00390000006879; -.
DR HOGENOM; CLU_064427_0_0_1; -.
DR InParanoid; Q96LK8; -.
DR OMA; HLFWANK; -.
DR OrthoDB; 1402495at2759; -.
DR PhylomeDB; Q96LK8; -.
DR TreeFam; TF338309; -.
DR PathwayCommons; Q96LK8; -.
DR SignaLink; Q96LK8; -.
DR BioGRID-ORCS; 124783; 10 hits in 1058 CRISPR screens.
DR GenomeRNAi; 124783; -.
DR Pharos; Q96LK8; Tdark.
DR PRO; PR:Q96LK8; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q96LK8; protein.
DR Bgee; ENSG00000184361; Expressed in left testis and 82 other tissues.
DR ExpressionAtlas; Q96LK8; baseline and differential.
DR Genevisible; Q96LK8; HS.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IBA:GO_Central.
DR GO; GO:0003779; F:actin binding; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IBA:GO_Central.
DR InterPro; IPR029297; SPATA32.
DR PANTHER; PTHR37338; PTHR37338; 1.
DR Pfam; PF15310; VAD1-2; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..384
FT /note="Spermatogenesis-associated protein 32"
FT /id="PRO_0000282906"
FT REGION 23..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 284..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 340..366
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..227
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..309
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 167
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66H17"
FT MOD_RES 170
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66H17"
FT VARIANT 142
FT /note="V -> M (in dbSNP:rs11651968)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.2"
FT /id="VAR_060279"
FT CONFLICT 112
FT /note="V -> A (in Ref. 1; BAB71683)"
FT /evidence="ECO:0000305"
FT CONFLICT 198..199
FT /note="TN -> SK (in Ref. 2; AAP97314)"
FT /evidence="ECO:0000305"
FT CONFLICT 218..219
FT /note="TT -> SK (in Ref. 2; AAP97314)"
FT /evidence="ECO:0000305"
FT CONFLICT 223
FT /note="A -> E (in Ref. 2; AAP97314)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="P -> A (in Ref. 2; AAP97314)"
FT /evidence="ECO:0000305"
FT CONFLICT 237
FT /note="D -> E (in Ref. 2; AAP97314)"
FT /evidence="ECO:0000305"
FT CONFLICT 243
FT /note="T -> H (in Ref. 2; AAP97314)"
FT /evidence="ECO:0000305"
FT CONFLICT 251
FT /note="A -> D (in Ref. 2; AAP97314)"
FT /evidence="ECO:0000305"
FT CONFLICT 343
FT /note="P -> S (in Ref. 1; BAB71683)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 384 AA; 42325 MW; 129700AB96ABFFD0 CRC64;
MGVTGAHGFP CCGKGSVEVA EMRDDLSQHQ IQEEQELEAD MLEQKPQLQV DLDLDPDPDP
DPELEIGQVP ALLESELYPA LKLEAELDTE ANSNEESDFE EPMQLVCKIE SVHSNMGLPT
PQTFRPWSLN SNCRSFTEEN HVSACHHSIS AQTSKHLFWA NKLIQASEHS LQRAINMQLN
NGSAGQPIRS PLREAIPTNA LCSEEQLQIP DAHSAPPTTS SQAPSPLLSS DLPPPIDLTE
LITFASSLAM ASSSRMDLPS LEHMMKAPPQ EALEPSTEPL LTTVEEREPE NHAETLPEKP
REARAPLKSW SQEDKNFAQS YFDFSKPGIK RATIKGQIQL LQPPATSPLL QGSKEDSVPP
GKEKENPLLV KIHFKLSAPT IPEK
