SPT32_MOUSE
ID SPT32_MOUSE Reviewed; 334 AA.
AC Q8C5V0;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Spermatogenesis-associated protein 32;
DE AltName: Full=Acrosome expressed protein 2;
GN Name=Spata32; Synonyms=Aep2, Vad1.2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INTERACTION WITH
RP SYNTAXIN-1 AND ACTB.
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=18621766; DOI=10.1093/molehr/gan041;
RA Lee K.F., Tam Y.T., Zuo Y., Cheong A.W., Pang R.T., Lee N.P., Shum C.K.,
RA Tam P.C., Cheung A.N., Yang Z.M., Yeung W.S., Luk J.M.;
RT "Characterization of an acrosome protein VAD1.2/AEP2 which is
RT differentially expressed in spermatogenesis.";
RL Mol. Hum. Reprod. 14:465-474(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Interacts with syntaxin-1 and ACTB.
CC {ECO:0000269|PubMed:18621766}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the testis and weakly in the
CC brain and heart. {ECO:0000269|PubMed:18621766}.
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DR EMBL; DQ118647; AAZ38360.1; -; mRNA.
DR EMBL; AK077068; BAC36591.1; -; mRNA.
DR EMBL; AL662804; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466558; EDL34182.1; -; Genomic_DNA.
DR EMBL; BC117072; AAI17073.1; -; mRNA.
DR EMBL; BC120775; AAI20776.1; -; mRNA.
DR CCDS; CCDS25515.1; -.
DR RefSeq; NP_808469.1; NM_177801.3.
DR AlphaFoldDB; Q8C5V0; -.
DR STRING; 10090.ENSMUSP00000099365; -.
DR PhosphoSitePlus; Q8C5V0; -.
DR PaxDb; Q8C5V0; -.
DR PRIDE; Q8C5V0; -.
DR ProteomicsDB; 257389; -.
DR Antibodypedia; 17631; 14 antibodies from 8 providers.
DR Ensembl; ENSMUST00000103076; ENSMUSP00000099365; ENSMUSG00000044787.
DR GeneID; 328019; -.
DR KEGG; mmu:328019; -.
DR UCSC; uc007ltz.1; mouse.
DR CTD; 124783; -.
DR MGI; MGI:3045340; Spata32.
DR VEuPathDB; HostDB:ENSMUSG00000044787; -.
DR eggNOG; ENOG502RU2T; Eukaryota.
DR GeneTree; ENSGT00390000006879; -.
DR HOGENOM; CLU_064427_0_0_1; -.
DR InParanoid; Q8C5V0; -.
DR OMA; HLFWANK; -.
DR OrthoDB; 1402495at2759; -.
DR PhylomeDB; Q8C5V0; -.
DR TreeFam; TF338309; -.
DR BioGRID-ORCS; 328019; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Spata32; mouse.
DR PRO; PR:Q8C5V0; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8C5V0; protein.
DR Bgee; ENSMUSG00000044787; Expressed in testis and 16 other tissues.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0003779; F:actin binding; ISO:MGI.
DR GO; GO:0007283; P:spermatogenesis; IBA:GO_Central.
DR InterPro; IPR029297; SPATA32.
DR PANTHER; PTHR37338; PTHR37338; 2.
DR Pfam; PF15310; VAD1-2; 2.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..334
FT /note="Spermatogenesis-associated protein 32"
FT /id="PRO_0000420265"
FT REGION 24..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..98
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 135
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66H17"
FT MOD_RES 138
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66H17"
SQ SEQUENCE 334 AA; 37222 MW; 5DDE15CC5FC2AA36 CRC64;
MGVTGISTFP CCGKDSVDIV ERQSDHHRHH HHHTHEENED EDTEVEAELP RTEPPPKVDP
ELGPVPQLEE MEPELPSKTT PETEGDSYTE SPEQQNYRME SLKPYEEEEM GGRYRSIPVQ
TSKHLFWSNK LIQASEHSLQ KALEKHHRSP QEKSISISQV YTECTQPPSS PPVSRPTTPT
AIGLADLINF ASSLAVASSS NMALPNLENM IKGTSEKSQN TSLDFCQPVQ AIKFAQATQI
TQISSEKQDE SPKSMAHKSW TRETRNVACS YLDINQAGLK TATIQGEVKF VQTPIASPQL
QEAKEDSVPG TKKGNPLLLK IHFKLSSPQP QRND
