SPT32_RAT
ID SPT32_RAT Reviewed; 348 AA.
AC Q66H17;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Spermatogenesis-associated protein 32;
DE AltName: Full=Acrosome expressed protein 2;
GN Name=Spata32; Synonyms=Aep2, Vad1.2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY, AND INTERACTION WITH SYNTAXIN-1 AND ACTB.
RX PubMed=18621766; DOI=10.1093/molehr/gan041;
RA Lee K.F., Tam Y.T., Zuo Y., Cheong A.W., Pang R.T., Lee N.P., Shum C.K.,
RA Tam P.C., Cheung A.N., Yang Z.M., Yeung W.S., Luk J.M.;
RT "Characterization of an acrosome protein VAD1.2/AEP2 which is
RT differentially expressed in spermatogenesis.";
RL Mol. Hum. Reprod. 14:465-474(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149; SER-152 AND THR-330, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- SUBUNIT: Interacts with syntaxin-1 and ACTB.
CC {ECO:0000269|PubMed:18621766}.
CC -!- TISSUE SPECIFICITY: Abundantly expressed in testes. Expressed in germ
CC cells, but not in Sertoli or Leydig cells of the adult testis.
CC Localized at the acrosomal region of the round and elongated spermatids
CC at stages VIII-X. {ECO:0000269|PubMed:18621766}.
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DR EMBL; AABR06065672; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH473948; EDM06261.1; -; Genomic_DNA.
DR EMBL; BC082079; AAH82079.1; -; mRNA.
DR RefSeq; NP_001005531.1; NM_001005531.1.
DR AlphaFoldDB; Q66H17; -.
DR STRING; 10116.ENSRNOP00000004371; -.
DR iPTMnet; Q66H17; -.
DR PhosphoSitePlus; Q66H17; -.
DR PaxDb; Q66H17; -.
DR GeneID; 287747; -.
DR KEGG; rno:287747; -.
DR UCSC; RGD:1359156; rat.
DR CTD; 124783; -.
DR RGD; 1359156; Spata32.
DR VEuPathDB; HostDB:ENSRNOG00000003275; -.
DR eggNOG; ENOG502RU2T; Eukaryota.
DR HOGENOM; CLU_064427_0_0_1; -.
DR InParanoid; Q66H17; -.
DR OMA; HLFWANK; -.
DR OrthoDB; 1402495at2759; -.
DR PhylomeDB; Q66H17; -.
DR TreeFam; TF338309; -.
DR PRO; PR:Q66H17; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Proteomes; UP000234681; Chromosome 10.
DR Bgee; ENSRNOG00000003275; Expressed in testis and 2 other tissues.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:RGD.
DR GO; GO:0003779; F:actin binding; IPI:RGD.
DR GO; GO:0007283; P:spermatogenesis; IEP:RGD.
DR InterPro; IPR029297; SPATA32.
DR PANTHER; PTHR37338; PTHR37338; 1.
DR Pfam; PF15310; VAD1-2; 2.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..348
FT /note="Spermatogenesis-associated protein 32"
FT /id="PRO_0000420266"
FT REGION 27..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 308..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..46
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 149
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 152
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 330
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 348 AA; 38580 MW; 4362AFB75127E6F7 CRC64;
MGVTGFSAFP CCGKNSVNIV ERKNDDYHHH HHPLEDNKDE DNEMGTELSS MKPPPKVDPD
PVPHLEDMVT ELTSTPIPET ENPEQQNYRI ESIKPYEEEL TTTNTFKPRG FNVNSTNKEE
EEVVCGHFRS TPVQTSKHLF WSNKLIQASE HSLQTALEKH HKSPGEKKSI SIAQVYTECT
QRPSSTQVSR TPTPTALGLA DLINFASSLA VASSSNMALP NLGTMIKGTS EKAQNTSLDF
CQPIQSIKFT QATQITQISS EKQDEPPEVM AHKSWTQETR NVACSYLDIN ESGLKKATIQ
GEVKFVQAPA TSPELQEDKD DSVPGTKKGT PILLKIHFKL SSPQAPEK