SPT33_HUMAN
ID SPT33_HUMAN Reviewed; 139 AA.
AC Q96N06; A8WFL2; B4DZN8;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Spermatogenesis-associated protein 33;
GN Name=SPATA33; Synonyms=C16orf55;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Teratocarcinoma, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH PPP3R1; PPP3R2; PPP3CA; PPP3CB AND PPP3CC.
RX PubMed=34446558; DOI=10.1073/pnas.2106673118;
RA Miyata H., Oura S., Morohoshi A., Shimada K., Mashiko D., Oyama Y.,
RA Kaneda Y., Matsumura T., Abbasi F., Ikawa M.;
RT "SPATA33 localizes calcineurin to the mitochondria and regulates sperm
RT motility in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC -!- FUNCTION: Plays an important role in sperm motility and male fertility
CC (By similarity). Required for sperm midpiece flexibility and for the
CC localization of sperm calcineurin to the mitochondria (By similarity).
CC Promotes mitophagy as well as acts as an autophagy mediator in male
CC germline cells (By similarity). Links damaged mitochondria to
CC autophagosomes via its binding to the outer mitochondrial membrane
CC protein VDAC2, as well as to key autophagy machinery component ATG16L1
CC (By similarity). {ECO:0000250|UniProtKB:Q8C624}.
CC -!- SUBUNIT: Interacts (via PQIIIT motif) with PPP3R1, PPP3R2, PPP3CA,
CC PPP3CB and PPP3CC (PubMed:34446558). Interacts with VDAC2 (By
CC similarity). Interacts with ATG16L1 (via WD repeats) (By similarity).
CC {ECO:0000250|UniProtKB:Q8C624, ECO:0000269|PubMed:34446558}.
CC -!- INTERACTION:
CC Q96N06; P48454: PPP3CC; NbExp=3; IntAct=EBI-17572815, EBI-2827192;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q8C624}. Nucleus {ECO:0000250|UniProtKB:Q8C624}.
CC Cytoplasm {ECO:0000250|UniProtKB:Q8C624}. Mitochondrion
CC {ECO:0000250|UniProtKB:Q8C624}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96N06-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96N06-2; Sequence=VSP_053288;
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DR EMBL; AK056168; BAB71109.1; -; mRNA.
DR EMBL; AK303024; BAG64150.1; -; mRNA.
DR EMBL; AC010538; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471184; EAW66714.1; -; Genomic_DNA.
DR EMBL; BC148299; AAI48300.1; -; mRNA.
DR CCDS; CCDS10983.1; -. [Q96N06-1]
DR CCDS; CCDS62012.1; -. [Q96N06-2]
DR RefSeq; NP_001258836.1; NM_001271907.1. [Q96N06-2]
DR RefSeq; NP_001258837.1; NM_001271908.1.
DR RefSeq; NP_001258838.1; NM_001271909.1.
DR RefSeq; NP_001258839.1; NM_001271910.1.
DR RefSeq; NP_694570.1; NM_153025.2. [Q96N06-1]
DR AlphaFoldDB; Q96N06; -.
DR BioGRID; 125847; 28.
DR IntAct; Q96N06; 2.
DR STRING; 9606.ENSP00000462996; -.
DR GlyGen; Q96N06; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q96N06; -.
DR PhosphoSitePlus; Q96N06; -.
DR BioMuta; SPATA33; -.
DR DMDM; 74732471; -.
DR EPD; Q96N06; -.
DR jPOST; Q96N06; -.
DR MassIVE; Q96N06; -.
DR MaxQB; Q96N06; -.
DR PaxDb; Q96N06; -.
DR PeptideAtlas; Q96N06; -.
DR PRIDE; Q96N06; -.
DR ProteomicsDB; 77439; -. [Q96N06-1]
DR TopDownProteomics; Q96N06-1; -. [Q96N06-1]
DR Antibodypedia; 64236; 20 antibodies from 10 providers.
DR DNASU; 124045; -.
DR Ensembl; ENST00000301031.8; ENSP00000301031.4; ENSG00000167523.15. [Q96N06-1]
DR Ensembl; ENST00000579310.6; ENSP00000462996.1; ENSG00000167523.15. [Q96N06-2]
DR GeneID; 124045; -.
DR KEGG; hsa:124045; -.
DR MANE-Select; ENST00000579310.6; ENSP00000462996.1; NM_001271907.2; NP_001258836.1. [Q96N06-2]
DR UCSC; uc002fnw.3; human. [Q96N06-1]
DR CTD; 124045; -.
DR DisGeNET; 124045; -.
DR GeneCards; SPATA33; -.
DR HGNC; HGNC:26463; SPATA33.
DR HPA; ENSG00000167523; Tissue enriched (testis).
DR MIM; 615409; gene.
DR neXtProt; NX_Q96N06; -.
DR OpenTargets; ENSG00000167523; -.
DR PharmGKB; PA142672262; -.
DR VEuPathDB; HostDB:ENSG00000167523; -.
DR eggNOG; ENOG502TF19; Eukaryota.
DR GeneTree; ENSGT00390000014546; -.
DR InParanoid; Q96N06; -.
DR OMA; EWGPYYR; -.
DR OrthoDB; 1625327at2759; -.
DR PhylomeDB; Q96N06; -.
DR TreeFam; TF337053; -.
DR PathwayCommons; Q96N06; -.
DR SignaLink; Q96N06; -.
DR BioGRID-ORCS; 124045; 19 hits in 1068 CRISPR screens.
DR ChiTaRS; SPATA33; human.
DR GenomeRNAi; 124045; -.
DR Pharos; Q96N06; Tdark.
DR PRO; PR:Q96N06; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q96N06; protein.
DR Bgee; ENSG00000167523; Expressed in sperm and 117 other tissues.
DR ExpressionAtlas; Q96N06; baseline and differential.
DR Genevisible; Q96N06; HS.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0097225; C:sperm midpiece; ISS:UniProtKB.
DR GO; GO:0097226; C:sperm mitochondrial sheath; ISS:UniProtKB.
DR GO; GO:0000423; P:mitophagy; ISS:UniProtKB.
DR InterPro; IPR027930; DUF4609.
DR PANTHER; PTHR38649; PTHR38649; 1.
DR Pfam; PF15382; DUF4609; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Autophagy; Cytoplasm; Mitochondrion; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..139
FT /note="Spermatogenesis-associated protein 33"
FT /id="PRO_0000282409"
FT REGION 1..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1..67
FT /note="Interaction with ATG16L1"
FT /evidence="ECO:0000250|UniProtKB:Q8C624"
FT REGION 68..139
FT /note="Interaction with VDAC2"
FT /evidence="ECO:0000250|UniProtKB:Q8C624"
FT REGION 97..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 86..91
FT /note="PQIIIT"
FT /evidence="ECO:0000250|UniProtKB:Q8C624"
FT COMPBIAS 20..50
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..80
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..124
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 94
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8C624"
FT VAR_SEQ 1..12
FT /note="MVTHAAGARTFC -> MGLSKSKEKPRKG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_053288"
FT VARIANT 45
FT /note="S -> L (in dbSNP:rs13329897)"
FT /id="VAR_050897"
SQ SEQUENCE 139 AA; 15461 MW; 30A94F08B8ED0C57 CRC64;
MVTHAAGART FCEEQKKGST YSVPKSKEKL MEKHSQEARQ ADRESEKPVD SLHPGAGTAK
HPPPAASLEE KPDVKQKSSR KKVVVPQIII TRASNETLVS CSSSGSDQQR TIREPEDWGP
YRRHRNPSTA DAYNSHLKE
