ID   SPT3_KLULA              Reviewed;         328 AA.
AC   O13472;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Protein SPT3;
GN   Name=SPT3; OrderedLocusNames=KLLA0E03212g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=9559549;
RX   DOI=10.1002/(sici)1097-0061(19980330)14:5<409::aid-yea237>3.0.co;2-x;
RA   Madison J.M., Winston F.;
RT   "Identification and analysis of homologues of Saccharomyces cerevisiae Spt3
RT   suggest conserved functional domains.";
RL   Yeast 14:409-417(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: SPT factors 3, 7 and 8 are required for the initiation of Ty
CC       transcription from the delta promoter. SPT3 regulates Ty1 as well as
CC       the mating factor genes. Interacts directly with TATA-binding protein.
CC       It is likely to be required for normal TBP function at a subset of RNA
CC       polymerase II-dependent promoters. {ECO:0000269|PubMed:9559549}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9559549}.
CC   -!- SIMILARITY: Belongs to the SPT3 family. {ECO:0000305}.
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DR   EMBL; AF005930; AAC49994.1; -; Genomic_DNA.
DR   EMBL; CR382125; CAG99180.1; -; Genomic_DNA.
DR   RefSeq; XP_454093.1; XM_454093.1.
DR   AlphaFoldDB; O13472; -.
DR   SMR; O13472; -.
DR   STRING; 28985.XP_454093.1; -.
DR   EnsemblFungi; CAG99180; CAG99180; KLLA0_E03279g.
DR   GeneID; 2894275; -.
DR   KEGG; kla:KLLA0_E03279g; -.
DR   eggNOG; KOG3902; Eukaryota.
DR   HOGENOM; CLU_038706_1_1_1; -.
DR   InParanoid; O13472; -.
DR   OMA; QFMFNEQ; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR   GO; GO:0000124; C:SAGA complex; IEA:EnsemblFungi.
DR   GO; GO:0046695; C:SLIK (SAGA-like) complex; IEA:EnsemblFungi.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:EnsemblFungi.
DR   GO; GO:0006325; P:chromatin organization; IEA:EnsemblFungi.
DR   GO; GO:0016573; P:histone acetylation; IEA:EnsemblFungi.
DR   GO; GO:0001403; P:invasive growth in response to glucose limitation; IEA:EnsemblFungi.
DR   GO; GO:0007124; P:pseudohyphal growth; IEA:EnsemblFungi.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   CDD; cd07978; TAF13; 1.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR003195; TFIID_TAF13.
DR   PANTHER; PTHR11380; PTHR11380; 1.
DR   Pfam; PF02269; TFIID-18kDa; 1.
DR   SUPFAM; SSF47113; SSF47113; 2.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..328
FT                   /note="Protein SPT3"
FT                   /id="PRO_0000072164"
FT   REGION          93..128
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        114..128
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   328 AA;  37452 MW;  38989ACD10F47940 CRC64;
     MSDKYKYRVE IQQMMFVSGE NNEPPVETTS LIEDIVRGQV VEILLQATRT ANCRGSKSIV
     PEDVIFLIRH DKAKVNRLRT YLSWKDLRKN AKDQDAQQAA AGGGAGDGSG PGGIDDDDAG
     KKDKDGAGVN MKVKKSSIKL PWELQFMFNE QPLENPDEDD MDDDEREANM ATLKRLKMAD
     DRTRGMTKEE YVHWSDCRQA SFTFRKSKRF KDWSGISQLI DSKPHDDVID ILGFLTFEIV
     CAITETALKI KTRYERIRSM KTQSQPQEYS VGTTRRKKRL FDGPDNVVNP LKPEHIEEAW
     RVLQTVNMKH RALSNYKGGR LSSRTVIM