ID   SPT4H_CAEBR             Reviewed;         120 AA.
AC   Q628A6; A8WMQ2;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Transcription elongation factor SPT4;
DE   AltName: Full=DRB sensitivity-inducing factor small subunit;
DE            Short=DSIF small subunit;
GN   Name=spt-4; ORFNames=CBG00462;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: May function as a component of the DRB sensitivity-inducing
CC       factor complex (DSIF complex), which regulates transcription elongation
CC       by RNA polymerase II. DSIF may enhance transcriptional pausing at sites
CC       proximal to the promoter, which may in turn facilitate the assembly of
CC       an elongation competent RNA polymerase II complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with spt-5 to form DSIF. DSIF interacts with RNA
CC       polymerase II and with the positive transcription elongation factor b
CC       complex (P-TEFb complex), which is composed of cdk-9 and cyclin-T (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SPT4 family. {ECO:0000305}.
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DR   EMBL; HE600978; CAP21757.1; -; Genomic_DNA.
DR   RefSeq; XP_002640905.1; XM_002640859.1.
DR   AlphaFoldDB; Q628A6; -.
DR   SMR; Q628A6; -.
DR   STRING; 6238.CBG00462; -.
DR   EnsemblMetazoa; CBG00462.1; CBG00462.1; WBGene00023846.
DR   GeneID; 8582899; -.
DR   KEGG; cbr:CBG_00462; -.
DR   CTD; 8582899; -.
DR   WormBase; CBG00462; CBP00080; WBGene00023846; Cbr-spt-4.
DR   eggNOG; KOG3490; Eukaryota.
DR   HOGENOM; CLU_138052_3_0_1; -.
DR   InParanoid; Q628A6; -.
DR   OMA; FDGMIAV; -.
DR   OrthoDB; 1617752at2759; -.
DR   Proteomes; UP000008549; Chromosome III.
DR   GO; GO:0032044; C:DSIF complex; IBA:GO_Central.
DR   GO; GO:0000993; F:RNA polymerase II complex binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006325; P:chromatin organization; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IBA:GO_Central.
DR   GO; GO:0032786; P:positive regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   CDD; cd07973; Spt4; 1.
DR   Gene3D; 3.30.40.210; -; 1.
DR   InterPro; IPR029040; RPABC4/Spt4.
DR   InterPro; IPR009287; Spt4.
DR   InterPro; IPR022800; Spt4/RpoE2_Znf.
DR   InterPro; IPR038510; Spt4_sf.
DR   PANTHER; PTHR12882; PTHR12882; 1.
DR   Pfam; PF06093; Spt4; 1.
DR   PIRSF; PIRSF025023; Spt4; 1.
DR   SMART; SM01389; Spt4; 1.
DR   SUPFAM; SSF63393; SSF63393; 1.
PE   3: Inferred from homology;
KW   Activator; Metal-binding; Nucleus; Reference proteome; Repressor;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..120
FT                   /note="Transcription elongation factor SPT4"
FT                   /id="PRO_0000210333"
FT   ZN_FING         15..35
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255"
FT   REGION          1..39
FT                   /note="Interaction with spt-5"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   120 AA;  13350 MW;  3D161A76833E4F88 CRC64;
     MAASIPSDLR NLRACLLCSL IKSVDAFQTD GCENCDEVLH LKGDEEKVYD CTSANYDGMI
     AAMSNDDSWV CKWQKMQRRV KGIYAISVSG SLPSNVVSDL KSMGVRYKAN QRDYSIQAKK