SPT4H_CAEEL
ID SPT4H_CAEEL Reviewed; 120 AA.
AC Q9TZ93;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Transcription elongation factor SPT4;
DE AltName: Full=DRB sensitivity-inducing factor small subunit;
DE Short=DSIF small subunit;
GN Name=spt-4; ORFNames=F54C4.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION.
RX PubMed=12183367; DOI=10.1101/gad.999002;
RA Shim E.Y., Walker A.K., Shi Y., Blackwell T.K.;
RT "CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for
RT transcription in the C. elegans embryo.";
RL Genes Dev. 16:2135-2146(2002).
CC -!- FUNCTION: May function as a component of the DRB sensitivity-inducing
CC factor complex (DSIF complex), which regulates transcription elongation
CC by RNA polymerase II. DSIF may enhance transcriptional pausing at sites
CC proximal to the promoter, which may in turn facilitate the assembly of
CC an elongation competent RNA polymerase II complex.
CC {ECO:0000269|PubMed:12183367}.
CC -!- SUBUNIT: Interacts with spt-5 to form DSIF. DSIF interacts with RNA
CC polymerase II and with the positive transcription elongation factor b
CC complex (P-TEFb complex), which is composed of cdk-9 and cyclin-T (cit-
CC 1.1 or cit-1.2) (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SPT4 family. {ECO:0000305}.
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DR EMBL; FO080712; CCD66087.1; -; Genomic_DNA.
DR PIR; T33605; T33605.
DR RefSeq; NP_497135.1; NM_064734.4.
DR AlphaFoldDB; Q9TZ93; -.
DR SMR; Q9TZ93; -.
DR BioGRID; 40448; 6.
DR DIP; DIP-25841N; -.
DR STRING; 6239.F54C4.2; -.
DR EPD; Q9TZ93; -.
DR PaxDb; Q9TZ93; -.
DR PeptideAtlas; Q9TZ93; -.
DR EnsemblMetazoa; F54C4.2.1; F54C4.2.1; WBGene00005014.
DR GeneID; 175172; -.
DR KEGG; cel:CELE_F54C4.2; -.
DR UCSC; F54C4.2; c. elegans.
DR CTD; 175172; -.
DR WormBase; F54C4.2; CE19891; WBGene00005014; spt-4.
DR eggNOG; KOG3490; Eukaryota.
DR GeneTree; ENSGT00390000018559; -.
DR HOGENOM; CLU_138052_3_0_1; -.
DR InParanoid; Q9TZ93; -.
DR OMA; FDGMIAV; -.
DR OrthoDB; 1617752at2759; -.
DR PhylomeDB; Q9TZ93; -.
DR Reactome; R-CEL-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-CEL-113418; Formation of the Early Elongation Complex.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-CEL-75955; RNA Polymerase II Transcription Elongation.
DR PRO; PR:Q9TZ93; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00005014; Expressed in embryo and 4 other tissues.
DR GO; GO:0032044; C:DSIF complex; IBA:GO_Central.
DR GO; GO:0000993; F:RNA polymerase II complex binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006325; P:chromatin organization; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IBA:GO_Central.
DR GO; GO:0032786; P:positive regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR CDD; cd07973; Spt4; 1.
DR Gene3D; 3.30.40.210; -; 1.
DR InterPro; IPR029040; RPABC4/Spt4.
DR InterPro; IPR009287; Spt4.
DR InterPro; IPR022800; Spt4/RpoE2_Znf.
DR InterPro; IPR038510; Spt4_sf.
DR PANTHER; PTHR12882; PTHR12882; 1.
DR Pfam; PF06093; Spt4; 1.
DR PIRSF; PIRSF025023; Spt4; 1.
DR SMART; SM01389; Spt4; 1.
DR SUPFAM; SSF63393; SSF63393; 1.
PE 3: Inferred from homology;
KW Activator; Metal-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..120
FT /note="Transcription elongation factor SPT4"
FT /id="PRO_0000210334"
FT ZN_FING 15..35
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 1..39
FT /note="Interaction with spt-5"
FT /evidence="ECO:0000250"
SQ SEQUENCE 120 AA; 13536 MW; E48D2A78789D5467 CRC64;
MSASVPADLR NLRACLLCSL VKSVESFQKE GCENCEDVLH LKGDEEKVYD CTSANYDGMI
AAMSNNESWV CKWQKMQRKV KGMYAISVSG VLPNNIVSEL KSLGVRYKPN QRDYSTQFKK
