ID   SPT4H_DANRE             Reviewed;         117 AA.
AC   Q6DGQ0;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Transcription elongation factor SPT4;
DE   AltName: Full=DRB sensitivity-inducing factor small subunit;
DE            Short=DSIF small subunit;
GN   Name=supt4h1; ORFNames=zgc:92824;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May function as a component of the DRB sensitivity-inducing
CC       factor complex (DSIF complex), which regulates transcription elongation
CC       by RNA polymerase II. Probably enhances transcriptional pausing at
CC       sites proximal to the promoter, which may facilitate the assembly of an
CC       elongation competent RNA polymerase II complex. Also acts to stimulate
CC       transcriptional elongation at low nucleotide concentrations. Regulation
CC       of transcriptional elongation by this protein is required for the
CC       expression of genes which control neuronal development (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with SUPT5H to form the DSIF complex. DSIF interacts
CC       with RNA polymerase II and with the positive transcription elongation
CC       factor b complex (P-TEFb complex), which is composed of CDK9 and
CC       cyclin-T (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SPT4 family. {ECO:0000305}.
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DR   EMBL; BC076290; AAH76290.1; -; mRNA.
DR   RefSeq; NP_001002509.1; NM_001002509.1.
DR   AlphaFoldDB; Q6DGQ0; -.
DR   SMR; Q6DGQ0; -.
DR   STRING; 7955.ENSDARP00000048842; -.
DR   PaxDb; Q6DGQ0; -.
DR   Ensembl; ENSDART00000179809; ENSDARP00000147232; ENSDARG00000113969.
DR   GeneID; 436782; -.
DR   KEGG; dre:436782; -.
DR   CTD; 6827; -.
DR   ZFIN; ZDB-GENE-040718-214; supt4h1.
DR   eggNOG; KOG3490; Eukaryota.
DR   GeneTree; ENSGT00390000018559; -.
DR   InParanoid; Q6DGQ0; -.
DR   OrthoDB; 1617752at2759; -.
DR   PhylomeDB; Q6DGQ0; -.
DR   TreeFam; TF300105; -.
DR   Reactome; R-DRE-113418; Formation of the Early Elongation Complex.
DR   Reactome; R-DRE-674695; RNA Polymerase II Pre-transcription Events.
DR   Reactome; R-DRE-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR   Reactome; R-DRE-6807505; RNA polymerase II transcribes snRNA genes.
DR   Reactome; R-DRE-75955; RNA Polymerase II Transcription Elongation.
DR   PRO; PR:Q6DGQ0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 5.
DR   Bgee; ENSDARG00000113969; Expressed in mature ovarian follicle and 21 other tissues.
DR   GO; GO:0032044; C:DSIF complex; ISS:UniProtKB.
DR   GO; GO:0000993; F:RNA polymerase II complex binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006325; P:chromatin organization; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IBA:GO_Central.
DR   GO; GO:0032786; P:positive regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   GO; GO:0034243; P:regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR   CDD; cd07973; Spt4; 1.
DR   Gene3D; 3.30.40.210; -; 1.
DR   InterPro; IPR029040; RPABC4/Spt4.
DR   InterPro; IPR009287; Spt4.
DR   InterPro; IPR022800; Spt4/RpoE2_Znf.
DR   InterPro; IPR038510; Spt4_sf.
DR   PANTHER; PTHR12882; PTHR12882; 1.
DR   Pfam; PF06093; Spt4; 1.
DR   PIRSF; PIRSF025023; Spt4; 1.
DR   SMART; SM01389; Spt4; 1.
DR   SUPFAM; SSF63393; SSF63393; 1.
PE   3: Inferred from homology;
KW   Activator; Metal-binding; Nucleus; Reference proteome; Repressor;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..117
FT                   /note="Transcription elongation factor SPT4"
FT                   /id="PRO_0000210330"
FT   ZN_FING         16..36
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255"
FT   REGION          1..40
FT                   /note="Interaction with SUPT5H"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   117 AA;  13232 MW;  AE6B05FE2DD4D9CE CRC64;
     MSLETVPKDL RHLRACLLCS LVKTIDQFEY DGCDNCESYL QMKGNREMVY ECTSSSFDGV
     IAMMSPEDSW VAKWQRIGNF KPGVYAVTVT GRLPPGVVRE LKSRGVIYRS RDTAVKT